1. Natl R&D Ctr Freshwater Fish Proc, Jiangxi Normal University, Nanchang 330022, China; 2. School of Life Science, Jiangxi Normal University, Nanchang 330022, China; 3. School of Health, Jiangxi Normal University, Nanchang 330022, China
摘要 We isolated a copper-resistant strain from a copper-contaminated area. The 18S and 26S rRNA sequencing showed it had the closest genetic relationship with Cryptococcus laurentii. It was found that the strain could grow normally in the medium with 15mmol/L Cu2+, and the maximum adsorption of copper was 29.36μmol/L/OD. In addition, the transcriptome analysis of the strains after copper stress showed that 126genes were up-regulated and 143genes were down-regulated; multiple genes related to amino acid metabolism, energy metabolism, and metal ion transport pathways were differentially expressed. At the same time, we found that copper stress blocked the glucose metabolism pathway, the expression of metabolism related genes decreased by 37% to 73%, which may be one of the important reasons why copper inhibited the growth of bacteria. The expression of heavy metal transport-related functional genes increased dramatically by 7 to 26 times in response to copper stress when induced by copper. The purpose of this study is to reveal the transcriptional response of the strain to copper stress and the metabolic pathways that may be involved in copper resistance, and to provide a basis for the application of microbial technology in copper pollution remediation.
Abstract：We isolated a copper-resistant strain from a copper-contaminated area. The 18S and 26S rRNA sequencing showed it had the closest genetic relationship with Cryptococcus laurentii. It was found that the strain could grow normally in the medium with 15mmol/L Cu2+, and the maximum adsorption of copper was 29.36μmol/L/OD. In addition, the transcriptome analysis of the strains after copper stress showed that 126genes were up-regulated and 143genes were down-regulated; multiple genes related to amino acid metabolism, energy metabolism, and metal ion transport pathways were differentially expressed. At the same time, we found that copper stress blocked the glucose metabolism pathway, the expression of metabolism related genes decreased by 37% to 73%, which may be one of the important reasons why copper inhibited the growth of bacteria. The expression of heavy metal transport-related functional genes increased dramatically by 7 to 26 times in response to copper stress when induced by copper. The purpose of this study is to reveal the transcriptional response of the strain to copper stress and the metabolic pathways that may be involved in copper resistance, and to provide a basis for the application of microbial technology in copper pollution remediation.
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