Proliferation characteristics of nitrifiers during enrichment culture of Anammox biofilm
LI Ren1,2, YU Li-fang1,3, LIU Tian1, LIU Ran1, YU Tao1, PENG Dang-cong1
1. School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; 2. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China; 3. Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：The proliferation characteristics of nitrifiers during enrichment culture of anaerobic ammonium oxidation (anammox) biofilm by increasing TN loading rate were investigated in an unsealed up-flow anaerobic fixed-bed biofilm (UAFB) reactor. The results showed that with the increasing surface ammonium loading rate (SALR), the ratio of ΔNO3-/ΔNH4+ in UAFB reactor gradually decreased from 0.76±0.11 to 0.42±0.10, indicating that high substrate concentration is more conducive to the culture of Anammox biofilm and not conducive to the proliferation of nitrite oxidizing bacteria (NOB). Moreover, the ammonia oxidation rate (AUR) and nitrite oxidation rate (NUR) of Anammox biofilm significantly increased during the culture of Anammox biofilm but eventually tend to stabilize. The results of fluorescence in-situ hybridization (FISH) and Illumina MiSeq further confirmed that the abundance of Ca. Brocadia and Ca. Jettenia was up to 33.51% and 3.02%, and the total abundance of ammonia oxidizing bacteria (AOB)-Nitrosomonas was just 0.78%. However, the relative abundance of Nitrospira was up to 2.32%, which might negatively affect the stable operation of PN/Anammox system.
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