以厌氧颗粒污泥作为接种污泥,在序批式活性污泥反应器(SBR)中驯化培养好氧颗粒污泥,通过好氧颗粒污泥胞外聚合物(EPS)提取和硫酸化多糖纯化方法的优化,探究好氧颗粒污泥SBR反应器中硫酸化多糖的生成变化.结果表明,好氧颗粒污泥SBR反应器运行效果稳定,对污水COD、NH4+-N、TN和TP的去除效果分别可达90%、97%、70%和93%以上.采用超声、水浴和碱处理的组合方法可有效地提取好氧颗粒污泥EPS和硫酸化多糖,经脱蛋白纯化后,硫酸化多糖可达56.6mg/g EPS,约为接种厌氧颗粒污泥中硫酸化多糖含量的1.4倍,表明好氧颗粒污泥有助于生成硫酸化多糖,为污水污泥有机质的高值转化和资源化提供了一种有效途径.
Abstract
In this study, the anaerobic granular sludge was used as seed sludge for domesticated cultivation of aerobic granular sludge in the sequencing batch reactors (SBR). By optimizing methods for extraction of the extracellular polymeric substances (EPS) and purification of the extracted sulfated polysaccharides, the production of the sulfated polysaccharides in SBR with aerobic granular sludge was investigated. The results showed that the running performance of SBR was steady and the removal of COD, NH4+-N, TN, and TP reached above 90%, 97%, 70%, and 93%, respectively. The EPS and sulfated polysaccharides could be favorably extracted from aerobic granular sludge with combined methods of ultrasonication, water-bath heating, and alkali treatment. After deproteinization and purification, the concentration of sulfated polysaccharides reached 56.6mg/g EPS, which was approximately 1.4 times of that contained in the anaerobic granular sludge. This result indicated that the aerobic granular sludge is conducive to produce the sulfated polysaccharides, providing an efficient route for high-value transformation and recycling of organic matters in wastewater sludge.
关键词
胞外聚合物 /
好氧颗粒污泥 /
硫酸化多糖 /
污水资源化
Key words
aerobic granular sludge /
extracellular polymeric substance /
sulfated polysaccharide /
wastewater resource recovery
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基金
国家自然科学基金资助项目(51978136);中央高校基本科研业务费专项资金资助项目(2232022A-10)
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