不同外源Cd(II)对<i>Pseudomonas aeruginosa</i> EPS的胁迫效应——产量、组分、吸附特性变化及其机制

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摘要以CdCl₂、CdSO₄和Cd（NO₃）₂为胁迫因子，比较了不同阴离子在胁迫/诱导过程中对Pseudomonas aeruginosa（P. aeruginosa）胞外聚合物（EPS）的产量和特性的影响以及对Cd（II）吸附性能的变化.研究发现，当Cd（NO₃）₂胁迫/诱导浓度为50mg/L时，EPS产量达到214.91mg/g VSS，其中蛋白质含量达到136.75mg/g VSS，较胁迫/诱导前增加了409.31%.此时EPS的吸附性能最好，对Cd（II）的平衡吸附量最大，较胁迫/诱导前增加了近一半.HPLC分析表明，EPS蛋白质中氨基酸的变化与EPS的吸附特性密切相关.3D-EEM、XPS和FTIR结果表明，EPS中C=O、C-N和N-H含量增加，有利于重金属的结合.竞争吸附实验发现，EPS对Cd（II）的亲和力高于Zn（II）.

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	陈丽瑶
	连泽阳
	宋卫锋
	戴文灿
	杨佐毅
	孙梦格
	黄祥武
	白晓燕

关键词 ：革兰氏阴性菌, 胞外聚合物, 蛋白质, 氨基酸, 竞争吸附, 重金属

Abstract：The effects of anions on the yield and characteristics of Pseudomonas aeruginosa EPS and its performance on Cd(II) adsorption were compared by using CdCl₂, CdSO₄ and Cd(NO₃)₂ as stress factors. The results showed that the maximum EPS yield of 214.91mg/g VSS (136.75mg/g VSS in protein content) was observed for Cd(NO₃)₂ at a concentration of 50mg/L, which was 409.31% higher than that before stress/induction. Under this condition, the maximum adsorption capacity for Cd(II) by the EPS was achieved, which was 50% higher than that before stress/induction. HPLC analysis showed that the changes of amino acids in EPS proteins were closely related to the adsorption characteristics of EPS. The results of 3D-EEM, XPS and FTIR showed that the content of C=O, C-N and N-H in EPS increased, which was conducive to the binding of heavy metals. Through competitive adsorption experiments, it was found that EPS has a higher affinity for Cd(II) than Zn(II).

Key words： gram-negative bacteria extracellular polymeric substances protein amino acid competitive adsorption heavy metals

收稿日期: 2023-05-11

PACS:

X172

基金资助:广东省自然科学基金资助项目（2021A15150558）

通讯作者: 宋卫锋,教授,weifengsong@gdut.edu.cn E-mail: weifengsong@gdut.edu.cn

作者简介: 陈丽瑶(1999-),女,广东揭阳人,广东工业大学硕士研究生,主要方向为胞外聚合物的研究.发表论文2篇.2695931749@qq.com.

引用本文:

陈丽瑶, 连泽阳, 宋卫锋, 戴文灿, 杨佐毅, 孙梦格, 黄祥武, 白晓燕. 不同外源Cd(II)对Pseudomonas aeruginosa EPS的胁迫效应——产量、组分、吸附特性变化及其机制[J]. 中国环境科学, 2024, 44(1): 537-547. CHEN Li-yao, LIAN Ze-yang, SONG Wei-feng, DAI Wen-can, YANG Zuo-yi, SUN Meng-ge, HUANG Xiang-wu, BAI Xiao-yan. Coercive effects of different exogenous Cd(II) on Pseudomonas aeruginosa EPS——Changes in yield, composition, adsorption characteristics and their mechanisms. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 537-547.

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