Effect of continuous shock load on the performance and microbial community responses of anaerobic sequencing biofilm batch reactor
WANG Ze1, YU Li-fang1, MA Zhi-xuan1, ZHENG Lan-xiang2,3, LIU Ran1, LIU Tian1
1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; 2. School of Ecological Environment, Ningxia University, Yinchuan 750021, China; 3. China Wine Industry Technology Institute, Yinchuan 750021, China
Abstract：A laboratory-scale anaerobic sequencing biofilm batch reactor (AnSBBR) was used for the digestion of winery wastewater, and its operational characteristics and microbial community structure under continuous shock load were investigated. The results showed the AnSBBR maintained high removal efficiency and stability during the first 13days (29~41d) of continuous shock load. With the increase of hydrogen partial pressure and VFAs contents, the stability decreased (VFA/TA=0.72), SMA decreased by 46.2%, HUR increased by 69.2%, and coenzyme F420concentration increased by 11.9%. Compared with the steady stage, only the Slime-EPS contents increased significantly (34.1%) at day 42, while at day 56, the TB-EPS and LB-EPS contents increased by 61.3% and 62.8%, respectively, and the PN/PS ratio increased by 197.8% and 126.0%. Continuous shock load induced EPS to release massive emergency protein products to resist stress, and the electroactive substance enhanced the electron transfer activity (35.5%). Illumina MiSeq has shown that the abundance of acidogenic bacteria such as Desulfovibrio, Ruminococcus, and Geobacter decreased, while the abundance of Methanobacterium increased from 32.2% to 50.9% under continuous shock load. The biofilm system responds to the impacts of continuous shock load by EPS layered secretion and enhanced the methanogenic pathway utilizing CO2 and H2.
王泽, 于莉芳, 马芷萱, 郑兰香, 刘然, 刘甜. 持续负荷冲击下AnSBBR运行性能及群落结构响应[J]. 中国环境科学, 2024, 44(1): 140-149.
WANG Ze, YU Li-fang, MA Zhi-xuan, ZHENG Lan-xiang, LIU Ran, LIU Tian. Effect of continuous shock load on the performance and microbial community responses of anaerobic sequencing biofilm batch reactor. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(1): 140-149.
Pérez-Pérez T, Pereda-Reyes I, Pozzi E, et al.Performance and stability of an expanded granular sludge bed reactor modified with zeolite addition subjected to step increases of organic loading rate and to organic shock load [J].Water Science and Technology, 2018,77(1):39-50.
[2]
Wang C, Liu Y, Wang C, et al.Biochar facilitates rapid restoration of methanogenesis by enhancing direct interspecies electron transfer after high organic loading shock [J].Bioresource Technology, 2021,320:124360.
[3]
Kim J, Lee C.Response of a continuous biomethanation process to transient organic shock loads under controlled and uncontrolled pH conditions [J].Water Research, 2015,73(15):68-77.
[4]
柴立,薛旭东,邓彦,等.冲击负荷对厌氧序批式反应器的影响及其恢复重建过程[J].环境污染与防治, 2013,35(9):54-57.Chai L, Xue X D, Deng Y, et al.Effect of shock load on the performance of anaerobic sequencing batch reactor and the recovery process [J].Environmental Pollution and Control, 2013,35(9):54-57.
[5]
Li J Z, Yan H, Chen Q Y, et al.Performance of anaerobic sludge and the microbial social behaviors induced by quorum sensing in a UASB after a shock loading [J].Bioresource Technology, 2021,330:124972.
[6]
Sentürk, E, Ince M, Engin G O.The effect of transient loading on the performance of a mesophilic anaerobic contact reactor at constant feed strength [J].Journal of Biotechnology, 2013,164(2):232-237.
[7]
Carvalhinha P P, Flores A, Rodrigues J, et al.AnSBBR applied to the treatment of metalworking fluid wastewater:Effect of organic and shock load [J].Applied Biochemistry and Biotechnology, 2010,162(6):1708-1724.
[8]
郑兰香,王泽,马芷萱,等.厌氧序批式生物膜反应器处理葡萄酒生产废水性能及菌群演替分析[J].环境工程学报, 2023,17(4):1387-1396.Zheng L X, Wang Z, Ma Z X, et al.Performance on treating winery wastewater by AnSBBR and analysis of the microbial community succession [J].Chinese Journal of Environmental Engineering, 2023, 17(4):1387-1396.
[9]
郑兰香.贺兰山东麓葡萄酒生产废水废物处理与资源化[M].北京:中国环境出版社, 2022.Zheng L X.Waste treatment and resource utilization of wine production wastewater in the eastern foothills of Helan Mountain [M].Beijing:China Environment Publishing Group, 2022.
[10]
Bolzonella D, Papa M, Da Ros C, et al.Winery wastewater treatment:A critical overview of advanced biological processes [J].Critical Reviews in Biotechnology, 2019,39(4):489-507.
[11]
Welz P J, Holtman G, Haldenwang R, et al.Characterisation of winery wastewater from continuous flow settling basins and waste stabilisation ponds over the course of 1year:implications for biological wastewater treatment and land application [J].Water Science and Technology, 2016,74(9):2036-2050.
[12]
Li D, Chen L, Liu X F, et al.Instability mechanisms and early warning indicators for mesophilic anaerobic digestion of vegetable waste [J].Bioresource Technology, 2017,245:90-97.
[13]
李杰,赵声兰,陈朝银.Folin-Ciocalteu比色法测定核桃青皮果蔬酵素总多酚含量[J].中国酿造, 2015,34(9):130-134.Li J, Zhao S L, Chen C Y.Determination of total polyphenol in walnut green husk fruit and vegetable compound ferment by Folin-Ciocalteu colorimetry [J].China Brewing, 2015,34(9):130-134.
[14]
Zhao Z Q, Zhang Y B, Yu Q L, et al.Communities stimulated with ethanol to perform direct inter species electron transfer for syntrophic metabolism of propionate and butyrate [J].Water Research, 2016, 102:475-484.
[15]
赵阳,李秀芬,堵国成,等.钴及其配合物对产甲烷关键酶的影响[J].水资源保护, 2008,24(2):82-85.Zhao Y, Li X F, Du G C, et al.Effects of cobalt and its complexation on the key enzymes in methanogenesis [J].Water Resources Protection, 2008,24(2):82-85.
[16]
Hou Y P, Peng D C, Xue X D, et al.Hydrogen utilization rate:A crucial indicator for anaerobic digestion process evaluation and monitoring [J].Journal of Bioscience and Bioengineering, 2014,117(4):519-523.
[17]
GB 5084-2021农田灌溉水质标准[S].GB 5084-2021 Standard for irrigation water quality [S].
[18]
Molina F, Ruiz-Filippi G, García, C, et al.Winery effluent treatment at an anaerobic hybrid USBF pilot plant under normal and abnormal operation [J].Water Science and Technology, 2007,56(2):25-31.
[19]
Macleod F A, Guiot S R, Costerton J W.Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor [J].Applied and Environmental Microbiology, 1990,56(6):1598-1607.
[20]
徐恒,夏瑜,王建兵,等.高氢分压下厌氧颗粒污泥演变过程研究[J].中国环境科学, 2019,39(11):4638-4645.Xu H, Xia Y, Wang J B, et al.Evolution of anaerobic granular sludge (AGS) under high H2partial pressure[J].China Environmental Science, 2019,39(11):4638-4645.
[21]
徐恒,汪翠萍,颜锟,等.颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究[J].中国环境科学, 2016,36(5):1435-1441.Xu H, Wang C P, Yan K, et al.Granule-based anaerobic biofilm enhances propionic acid degradation under high H2partial pressure [J].China Environmental Science, 2016,36(5):1435-1441.
[22]
Davididou K, Frontistis Z.Advanced oxidation processes for the treatment of winery wastewater:A review and future perspectives [J].Journal of Chemical Technology and Biotechnology, 2021,96(9):2436-2450.
[23]
Li L, Peng X Y, Wang X M, et al.Anaerobic digestion of food waste:A review focusing on process stability [J].Bioresource Technology, 2018,248:20-28.
[24]
Vital-Jacome M, Cazares-Granillo M, Carrillo-Reyes J, et al.Characterization and anaerobic digestion of Mexican highly concentrated wine by-products and effluents [J].Water Science and Technology, 2020,81(1):190-198.
[25]
Le C, Stuckey D C.Influence of feed composition on the monomeric structure of free bacterial extracellular polysaccharides in anaerobic digestion [J].Environmental Science and Technology, 2017,51(12):7009-7017.
[26]
Thomas S, Derlon N, Dueholm M S, et al.Extracellular polymeric substances of biofilms:Suffering from an identity crisis [J].Water Research, 2019,151(15):1-7.
[27]
Zhu L, Lv M L, Dai X, et al.Role and significance of extracellular polymeric substances on the property of aerobic granule [J].Bioresource Technology, 2012,107(9):46-54.
[28]
Xiao Y, Zhang E, Zhang J, et al.Extracellular polymeric substances are transient media for microbial extracellular electron transfer [J].Science Advances, 2017,3(7):1700623.
[29]
Vasquez J, Nakasaki K.Effects of shock loading versus stepwise acclimation on microbial consortia during the anaerobic digestion of glycerol [J].Biomass and Bioenergy, 2016,86:129-135.
[30]
Braz G H R, Fernandez-Gonzalez N, Lema J M, et al.The time response of anaerobic digestion microbiome during an organic loading rate shock [J].Applied Microbiology and Biotechnology, 2018,102:10285-10297.
[31]
Wang C Q, Liu Y, Jin S, et al.Responsiveness extracellular electron transfer (EET) enhancement of anaerobic digestion system during start-up and starvation recovery stages via magnetite addition [J].Bioresource technology, 2018,272:162-170.
[32]
班巧英,岳立峰,李建政,等.萘厌氧降解菌群的富集及氧化还原介体的强化[J].中国环境科学, 2020,40(7):3150-3155.Ban Q Y, Xue L F, Li J Z, et al.Enrichment of naphthalene anaerobic degrading bacterial consortium and enhancement by redox mediators [J].China Environmental Science, 2020,40(7):3150-3155.
[33]
张虹,李蕾,彭韵,等.氨氮对餐厨垃圾厌氧消化性能及微生物群落的影响[J].中国环境科学, 2020,40(8):3465-3474.Zhang H, Li L, Peng Y, et al.Effects of ammonia on anaerobic digestion of food waste:Process performance and microbial community [J].China Environmental Science, 2020,40(8):3465-3474.
[34]
Mario P, Gregor D Z, Marina T, et al.Biogas production from brewery spent grain as a mono-substrate in a two-stage process composed of solid-state anaerobic digestion and granular biomass reactors [J].Journal of Cleaner Production, 2017,166(10):519-529.
[35]
Zhao Z Q, Wang J F, Li Y, et al.Why do DIETers like drinking:Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol [J].Water Research, 2020,171:115425.
