Anammox菌干粉菌剂制备与保藏技术

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摘要采用真空干燥法制备anammox菌干粉菌剂，并在4℃恒温真空避光环境中进行为期30d的保藏.同时筛选了烘干保护剂的种类并优化了影响因素，以此对保藏后的anammox菌复壮性能进行研究.实验结果表明：在聚乙烯醇、羧甲基纤维素钠、海藻酸钠、海藻糖和去离子水5种保护剂中，最适宜的保护剂为海藻酸钠，与之对应的烘干温度和保护剂浓度的优化结果为60℃和3%.保藏后的anammox菌经过连续30d的复壮培养，anammox菌活性可恢复至新鲜菌液活性的41%.

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	张千
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关键词 ：厌氧氨氧化, 烘干保护剂, 海藻酸钠, 条件优化

Abstract：The dry powder agent of anammox bacteria was prepared by vacuum drying method and stored at 4℃ for 30days which was protected from light and air. Meanwhile, the drying protective agent and correlated influence factors were screened in order to study the rejuvenative performance of the preserved anammox bacteria. Results showed that sodium alginate was the most suitable drying protective agent among these alternative protective agents of polyving akohol, carboxyl methyl cellulose, sodium alginate, trehalose and deionized water. Correspondingly, the optimized drying temperature and concentration of sodium alginate were 60℃ and 3% respectively. After rejuvenation of the preserved anammox bacteria for 30days, the activity of anammox bacteria had recovered to 41% compared to the fresh bacteria.

Key words： anammox drying protective agent sodium alginate optimal condition

收稿日期: 2017-04-01

X703.5

基金资助:国家自然科学基金资助项目（21177019）

通讯作者: 杨凤林,教授,yangfl@dlut.edu.cn E-mail: yangfl@dlut.edu.cn

作者简介: 张千(1990-),女,河北保定人,大连理工大学硕士研究生,主要从事水污染控制研究.

引用本文:

张千, 徐晓晨, 王超, 王晓静, 杨凤林. Anammox菌干粉菌剂制备与保藏技术[J]. 中国环境科学, 2017, 37(12): 4630-4636. ZHANG Qian, XU Xiao-chen, WANG Chao, WANG Xiao-jing, YANG Feng-lin. Technique of preparation and preservation of dry powder agent of anammox bacteria. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(12): 4630-4636.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2017/V37/I12/4630

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