Comparison and assessment of haze particles health risks from different sources in city
JIANG Jin-xiao1, HE Jian-bo1, CHEN Bin1, LI Ning1, CHEN Fei-fei1, SHAN Xiao-dong1, TANG Juan1, ZHANG Hang-jun1,2
1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310016, China;
2. Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310016, China
To evaluate the health risks induced by haze particles sampled from three different areas. The present study assessed the effects of haze particles on electrical impedance and autophagy factors of the tracheal epithelial cells. Rat tracheal epithelial (RTE) cells were exposed to haze particles collected from three different areas, including residential area (I), traffic area (Ⅱ), and chemical industry park (Ⅲ) in the megacity Hangzhou, China. The particle concentration was treated and designed as 100mg/L and then a period of 24h exposure was given to cells in treatment groups. The electronic impedance and the time needed for the recovery of electricity damage were determined by ECIS (Electric Cell-substrate Impedance Sensing). Western blot was used to analyze the protein expressions of p62, Atg5, Atg7, Beclin1, LC3B and mTOR to compare the effects of different haze particles on RTE cells autophagy. The results showed that the recovery time of cell injury was prolonged by 34.6%, 63.2% and 78.0%, respectively, when compared with the control group. The expression of Atg5, Atg7, Beclin1and LC3B proteins increased, whereas that of p62protein significantly decreased. The expression of mTOR related proteins decreased significantly by 4.38%, 3.34% and 2.36%, respectively. Compared with the control group, the expression of p-mTOR decreased by 24.2% in group I, 37.0% in group Ⅱ and 60.9% in group Ⅲ. All the results suggested that haze particles collected from different functional areas could induce various toxic damages on RTE cells such as reduction of growth speed, impairment of revovery ability, and increased authophagy protein expression. The haze particles from chemical industrial areas showed more toxic effects than those from residential areas and traffic areas. The cytotoxicity of haze particles from different sources is obviously different The determination of cell electrical injury recovery time and the detection of autophagy-related proteins can provide a rapid biological method for health risk assessment of haze particles.
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