Microbial characteristics of bulking sludge in high-solids anaerobic digestion of kitchen waste
HE Qin1,2, LI Lei1, QU Li1, ZHAO Xiao-fei1, WU Di1, PENG Xu-ya1
1. Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; 2. Department of Environmental Science and Engineering, China West Normal University, Nanchong 637002, China
Abstract：A plug-flow reactor (PFR)(R1) and a completely-stirred tank reactor (CSTR)(R2) were operated under mesophilic temperature (37±1℃) for high-solids digestion of kitchen waste to investigate the microbial community characteristics before and after sludge-bulking using MiSeq high-throughput sequencing technology.The results showed that the archaeal community structure changed little after sludge-bulking,and acetoclastic methanogen Methanosaeta and mixotrophic methanogen Methanosarcina dominated in both reactors.There was a marked increase in the relative abundance of bacteria genera that might be related to sludge bulking.Those proliferated bacteria genera are those capable of producing biosurfactants (Corynebacterium,Lactobacillus,etc.) and those containing mycolic acids in cell walls (Actinomyces,Corynebacterium,etc.).In addition,volatile fatty acids (VFAs) and total ammonia nitrogen (TAN) were accumulated in these reactors before sludge-bulking.Accordingly,bacteria that can contribute to the accumulation of VFAs (Petrimonas,Anaerosalibacter and Fastidiosipila,etc.) and TAN (Proteiniphilum,Tepidimicrobium and Aminobacterium,etc.) were observed to proliferate.
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