Effects of different speices of bacterial-feeding nematodes on soil microorganisms in oil-contaminated soils
ZHOU Ji-Hai1,2, TANG Jia-Jie1, GAO Ru-ru1, YANG Hong-Fei2, CHENG Hong1, LIU Jia-Li1, YUAN Ying-Hong1
1. Jiangxi Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology, Nanchang Institute of Technology, Nanchang 330099, China;
2. Anhui Provincial Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China
The effects of different species of bacterial-feeding nematodes on soil microbial activity and diversity in oil-contaminated soil were studied in an effort to provide a preliminary theoretical basis and a new research perspective for the bioremediation of oil-contaminated soil. Five treatments were set up in our experiment:nematode-free soil (NFS), oil-contaminated soil (SP), oil-contaminated soil + Caenorhabditis elegans (SPN1); oil contaminated soil + Cephalobus persegnis (SPN2); oil contaminated soil + Rhabditis marina (SPN3). After 168d of the experiment, the findings were as follows:the soil basal respiration and microbial biomass carbon of SP increased due to oil pollution, while the activities of urease and sucrase decreased, and the richness, diversity index and evenness index of microorganisms decreased too. Compared with SP, the basal respiration of SPN1, SPN2 and SPN3 inoculated with nematodes increased, while the microbial biomass carbon decreased by 34.59% to 72.48%, and the sucrase activity increased by 15.66% to 22.89%. Relative to 0d, the microbial richness (S) of SP, SPN1, SPN2 and SPN3 decreased by 5, 7.25, 2.5 and 9.75, respectively, and the shannon-wiener index (H') decreased by 0.18, 0.15, 0.15 and 0.23. The results demonstrated that different species of bacterial-feeding nematodes helped to improve the microbial activity in oil-polluted soil and enhance the oil degradation through predation.
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