The performance of UASB at startup and the quantitative analysis of syntrophic propionate-oxidizing bacteria
ZHANG Li-guo1, QIN Yan1, LI Jian-zheng2, BAN Qiao-ying1, Liu Qi1
1. College of Environment and Resource, Shanxi University, Taiyuan 030006, China;
2. School of Environment, Harbin Institute of Technology, Harbin 150090, China
To reveal the relationship between the performance of upflow anaerobic sludge bed (UASB) reactor and syntrophic propionate-degrading consortia at startup, the operational characteristics during corn starch processing wastewater treatment in a UASB were investigated. The succession of syntrophic propionate-degrading consortia (propionate-oxidizing bacteria and methanogens) at startup was analyzed by quantitative real-time fluorescence polymerase chain reaction (qPCR). COD removal reached 91.9% after 38d operation under influent COD of 2000mg/L and hydraulic retention time (HRT) of 24h conditions. When HRT was shorten to 8h by stepwise, the specific methanogenic rate achieved 315LCH4/(kg COD·d) and the granular sludge with good settling performance was formed. qPCR analysis showed that five identified species of propionate propionate-oxidizing bacteria existed in the UASB reactor. Pelotomaculum propionicum was the major propionate-oxidizing bacteria in inoculation sludge, accounting for 45.7% in detectable propionate-oxidizing bacteria. Its number was reduced as HRT shorting. However, the quantity of Syntrophobacter sulfatireducens and S. wolinii were increased as HRT decrease. The maximum with 1.3×103 and 5.5×103 16S rDNA copies/ng DNA was reached in the end of startup, becoming the dominant propionate-oxidizing bacteria in the granular sludge. Methanobacterium and Methanosarcina were the dominant hydrogenotrophic and acetotrophic methanogens in the inoculation sludge. Their numbers were gradually reduced as HRT decrease. The quantity of Methanospirillum and Methanosaeta were increased, being the predominant methanogens in the granular sludge.
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ZHANG Li-guo, QIN Yan, LI Jian-zheng, BAN Qiao-ying, Liu Qi. The performance of UASB at startup and the quantitative analysis of syntrophic propionate-oxidizing bacteria. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(2): 615-622.
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