Screening of polyamine-producing bacteria and their effects on reducing Cd and Pb accumulation in Ipomoea aquatica
HAN Hui1,2, CAI Hong1, WANG Xiao-yu1, LIU Hui-xian1, CHEN Zhao-jin1,2, YAO Lun-guang1,2
1. Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
2. Henan Provincal Academician Workstation of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project. Nanyang 473061, China
Polyamine-production screening media and ultra performance liquid chromatography (UPLC) were used to isolate polyamine-producing bacteria from Cd- and Pb-pollutedrhizospheresoil ofIpomoea aquatica. The effects of functional strains on the growth, polyamine content, antioxidant enzyme activity and Cd and Pbaccumulation of I.aquatica were studied by hydroponics experiment. Results showed that nine high polyamine-production strains were isolated from the rhizosphere soil of I.aquatica. Among them, srainEnterobacter bugandensisYX6secreted spermine and spermidine, strain Klebsiellaquasivariicola YX8secreted putrescine. The adsorption rates for strains YX6and YX8to Cd and Pb were more than 80% in LB medium solution.Hydroponics experiment showed that strain YX6 and YX8significantly increased the content of polyamines (29.3%~180%) and the activities of superoxide dismutase (18.5%~42.7%) and peroxidase (46.2%~100%) in I.aquatica shoots and roots, and significantly increased the dry weight of shoots (21.2%~40.9%) and roots (43.3%~68.2%), and reduced the Cd (45.8%~69.5%) and Pb (42.5%~63.6%) contents in I.aquatica shoots and roots.Polyamine-production bacteria E. bugandensisYX6and K. quasivaricolaYX8not only improved the biomass and antioxidant enzyme activity of I.aquatica, but also reduced the absorption of Cd and Pb by I.aquatica, which provides bacterial resources for the safe utilization of heavy metals in farmland and the reduction of heavy metals content in vegetables.
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HAN Hui, CAI Hong, WANG Xiao-yu, LIU Hui-xian, CHEN Zhao-jin, YAO Lun-guang. Screening of polyamine-producing bacteria and their effects on reducing Cd and Pb accumulation in Ipomoea aquatica. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2692-2699.
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