Responseof microbial community structure of crops under the pollution of sulphadiazine on the rhizosphere microinterface
JIN Cai-xia1, HUANG Liu-qun1, WEI Shan1, ZOU Wei1, ZHANG Xing-li1, SUN Rui-lian2
1. Key Laboratory for Yellow River and HuaiHe River Water Environmental and Pollution Control, Ministry of Education, Henan Key Laboratory of Environmental Pollution Control, School of Environment, Henan Normal University, Xinxiang 453007, China;
2. Environment Research Institute, Shandong University, Qingdao 266237, China
In order to study the response of two crops to the microbial community structure of rhizosphere in different growth periods under sulfa veterinary drug stress.The amount of phospholipid fatty acids in soils at different micro-interfaces in rhizosphere was determined by rhizobox training experiments. The changes of microbial community structure in different spatial micro-interfaces of domesticated maize and wheat rhizosphere soil under sulfadiazine stress were studied. The results showed that the high concentration of SD had an inhibitory effect on rhizosphere microbial activity, and microbial biomass at different micro-interfaces in rhizosphere was different. The microbial biomass of the rhizosphere 3mm and root chamber was the largest at the micro-interface of the rhizosphere, and the difference between the two was not significant. Different microorganisms had different sensitivity to rhizosphere effects, and rhizosphere effects of bacteria, Gram-positive bacteria, Gram-negative bacteria, cyanobacteria and sulfate-reducing bacteria were obvious. Under the action of SD stress and root exudates, the microbial community structure of rhizosphere soil changed significantly at different micro-interfaces. In the rhizosphere soil of wheat, the ratio of F/B increased with the increase of SD concentration in soil. Under the high concentration (5mg/kg), the F/B ratio was the largest (0.74), which was significantly different from the control (P < 0.05), indicating that the soil pollution remediation ability was enhanced, the sat/mono ratio was decreased at high concentration, and the microbial community structure was transformed in the direction favorable for SD degradation. The rhizosphere effect was beneficial to SD degradation. Under the action of 1mg/kg SD, the degradation rate in wheat rhizosphere soil was 7.01%, while the degradation rate in non-rhizosphere soil was only 2.49%, and the rhizosphere effect of maize was stronger than wheat.
金彩霞, 黄刘群, 魏闪, 邹威, 张杏丽, 孙瑞莲. 磺胺嘧啶胁迫下作物根际微生物群落结构响应[J]. 中国环境科学, 2020, 40(6): 2708-2714.
JIN Cai-xia, HUANG Liu-qun, WEI Shan, ZOU Wei, ZHANG Xing-li, SUN Rui-lian. Responseof microbial community structure of crops under the pollution of sulphadiazine on the rhizosphere microinterface. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2708-2714.
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