Enrichment of naphthalene anaerobic degrading bacterial consortium and enhancement by redox mediators
BAN Qiao-ying1, YUE Li-feng1, LI Jian-zheng2, YU Min1, ZHANG Li-guo1
1. College of Environment and Resource, Shanxi University, Taiyuan 030006, China; 2. School of Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract：To reveal the catalytic effect of redox mediators (ROMs) on naphthalene anaerobic degradation, a mesophilic naphthalene anaerobic degrading bacterial consortium was enriched using naphthalene as sole carbon source. The bacterial community structure of inoculated sludge and enriched consortium were analyzed by Illumina MiSeq. The catalytic effect of anthraquinone-2,6-disulfonate (AQDS), anthraquinone-2-sulfonate (AQS) and humic acid on naphthalene degradation was also investigated. Illumina MiSeq sequencing revealed that Pseudomonas, Thauera, and Georgfuchsia were the dominant genera in the enriched consortium. Their relative abundance was 52.4%, 13.8% and 17.6%, respectively. Under the inoculated sludge of 0.23g/L and initial naphthalene of 10mg/L conditions, the naphthalene removal was 64.0% within 9 days by the enriched consortium. The result of enhancement experiment showed that ROMs could enhance the anaerobic degradation of naphthalene. Among them, the enhancement effect of AQDS was higher than other ROMs. At the AQDS of 0.8mmol/L, naphthalene removal reached 92.0% on the 7th day, which was higher than that of the control by1.2times. In addition, this study also investigated the effect of nitrate on the degradation of naphthalene. The results showed that the naphthalene removal showed a decreasing trend after increasing with nitrate increase from 0.2 to 0.8g/L. When the concentration of NaNO3 was 0.6g/L, the naphthalene removal reached 91.0% on the 6th day, which is higher than control by 15.2%. Therefore, appropriate nitrate would enhance the catalytic effect of ROMs on naphthalene degradation.
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