Decolorization exploration of azo dye by Aspergillus flavus A5p1: characteristics and biodegradation pathway
CHENG Ning1, LI Qing-yun1,2, LIU You-yan1,2
1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China; 2. Guangxi Key Laboratory of Biorefinery, Nanning 530003, China
Abstract：Aspergillus flavus strain A5p1 was used as a biological material to study its broad decolorization spectrum and decolorization characteristics and degradation products with the azo dye Direct Blue 71 (DB71) as the model substrate. The results showed that the decolorization efficiency of 15 kinds of dyes ranged from 61.7% to 100.0% with the 100mg/L dye concentration. There might be a synergistic effect of biosorption and biodegradation on the decolorization. The optimum decolorization condition of A5p1 for azo dye DB71 was pH 7.0, temperature 30℃, dye concentration 300mg/L, and sucrose as a carbon source. Enzyme analysis revealed that glucose oxidase and manganese peroxidases were involved in the degradation process. FTIR, GC-MS and LC-MS analyses indicated that the intermediates of azo dye degradation included naphthalene amine, naphthalene diazonium, 2-hydroxy-6-oxal-yl-benzoic acid and 1-naphthol.
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