Mechanism for electric field enhancing remediation of uranium and cadmium co-contaminated soil by Macleaya cordata
HU Nan1, ZHU Ruo-nan1, CHENG Hao2, TAN Guo-chi2, DONG Xue2, ZHANG Hui1, MA Jian-hong1, WANG Yong-dong2, DING De-xin1
1. Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Henyang 421001, China; 2. Hunan Province Key Laboratory of Green Development Technology for Exetremely Low Grad Uranium Resources, University of South China, Henyang 421001, China
Abstract：Pot experiments were conducted to investigate variations in biomass, the accumulation performance of uranium and cadmium, and the antioxidant enzyme activities of Macleaya cordata, and in organic acid content, the speciation of uranium and cadmium, the valence state of uranium of the root, and the structure of microbe community in its rhizosphere soil under the application of DC electric field at 0.3V/cm. The results showed that the total biomass of Macleaya cordata was increased by 15.33%~29.88%, the accumulation coefficients of uranium and cadmium in DC+U and DC+Cd treatment groups were increased by 90.84% and 93.33% The proportion of available uranium and cadmium in rhizosphere soil increased, respectively, and the contents of oxalic acid, tartaric acid, succinic acid, malic acid, and lactic acid were increased by 18.36%~45.31%, 58.62%~503.22%, 15.71%~118.99%, 12.34%~123.27%, and 25.97%~36.05%, respectively, the activities of POD and GSH-PX were increased by 13.63%~34.82% and 9.70%~28.64%, respectively. The proportions of bioavailable uranium and cadmium in the rhizosphere soil were increased significantly. Moreover, the stable U(IV) in the root of Macleaya cordata was transformed to mobile U(VI), which could easily migrate from the underground part to the aboveground part of the plant, and the proportions of Acidobacteria and Ascomycota in the rhizosphere microbial community were increased, and they promoted Macleaya cordata's tolerance to and accumulation of uranium and cadmium mainly by improving enzyme activity.
胡南, 朱若南, 成浩, 谭国炽, 董雪, 张辉, 马建洪, 王永东, 丁德馨. 电场强化博落回修复铀镉复合污染土壤机理[J]. 中国环境科学, 2020, 40(10): 4457-4467.
HU Nan, ZHU Ruo-nan, CHENG Hao, TAN Guo-chi, DONG Xue, ZHANG Hui, MA Jian-hong, WANG Yong-dong, DING De-xin. Mechanism for electric field enhancing remediation of uranium and cadmium co-contaminated soil by Macleaya cordata. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(10): 4457-4467.
裴晶晶,胡南,张辉,等.铀尾矿中不同形态铀释放的影响因素及其相关性[J]. 中国环境科学, 2019,39(7):3073-3080. Pei J J, Hu N, Zhang H, et al. An analysis of influencing factors on the release of different species of uranium from uranium tailings and their correlation[J]. China Environmental Science, 2019,39(7):3073-3080.
景称心,孔秋梅,冯志刚.中国南方某铀尾矿库周缘土壤重金属污染研究[J]. 中国环境科学, 2020,40(1):338-349. Jing C X, Kong Q M, Feng Z G. Heavy metal pollution in a uranium mining and metallurgy area in South China[J]. China Environmental Science, 2020,40(1):338-349.
周书葵,肖江,刘迎久,等.电动修复过程中电解质浓度对U(Ⅵ)迁移和能耗的影响[J]. 中国环境科学, 2019,39(12):5228-5239. Zhou S K, Xiao J, Liu Y J, et al. Effect of electrolyte concentration on U(VI) migration behavior and energy utilization in electrokinetic remediation process[J]. China Environmental Science, 2019,39(12):5228-5239.
Peppicelli C, Cleall P, Sapsford D, et al. Changes in metal speciation and mobility during electrokinetic treatment of industrial wastes:Implications for remediation and resource recovery[J]. Science of the Total Environment, 2018,(624):1488-1503.
Cameselle C, Chirakkara R A, Reddy K R. Electrokinetic-enhanced phytoremediation of soils:status and opportunities[J]. Chemosphere, 2013,93(4):626.
Li C, Hu N, Ding D, et al. Phytoextraction of uranium from contaminated soil by Macleaya cordata before and after application of EDDS and CA[J]. Environmental Science and Pollution Research, 2015,22(8):6155-6163.
Nie J, Liu Y G, Zeng G M, et al. Cadmium accumulation and tolerance of Macleaya cordata:a newly potential plant for sustainable phytoremediation in Cd-contaminated soil[J]. Environmental Science and Pollution Research, 2016,23(10):10189-10199.
Sha Y H, Hu N, Wang Y D, et al. Enhanced phytoremediation of uranium contaminated soil by artificially constructed plant community plots[J]. Journal of Environmental Radioactivity, 2019,208-209:106036.
陈威,胡南,陈可,等.博落回和竹柳间作修复铀污染土壤的研究[J]. 原子能科学技术, 2018,52(10):1748-1755. Chen W, Hu N, Chen K, et al. Study on the remediation of uranium contaminated soil by intercropping with bamboo and willow[J]. Atomic Energy Science and Technology, 2008,52(10):1748-1755.
Li G Y, Hu N, Ding D X, et al. Screening of plant species for phytoremediation of uranium, thorium, barium, nickel, strontium and lead contaminated soils from a uranium mill tailings repository in South China[J]. Bulletin of Environmental Contamination and Toxicology, 2011,86:646-652.
李倩.典型有色金属矿区Cd、Pb、As复合污染土壤稳定化修复试验研究[J]. 湖南有色金属, 2020,36(1):55-60. Li Q. Experimental study on stabilization and remediation of soil contaminated by Cd, As and Pb in typical nonferrous metal mining areas[J]. HuNan Nonferrous Metals, 2020,36(1):55-60.
Nie X Q, Dong F Q, Liu M X, et al. Removel of uranium from aqueous solutions by Spirodela Punctata as the mechanism of biomineralization[J]. Procedia Environmental Sciences, 2016,31:382-391.
Duquène L, Vandenhove H, Tack F et al. Enhanced phytoextraction of uranium and selected heavy metals by Indian mustard and ryegrass using biodegradable soil amendments[J]. Science of the Total Environment, 2009,407(5):1496-1505.
张婷.几种作物根际与非根际土壤养分含量差异探析[D]. 重庆:西南大学, 2012. Zhang T. Nutrient content difference analysis about several crops' rhizosphere and non rhizosphere Soil[D]. Chongqing:Southwest University, 2012.
Vandenhove H, Vanhoudt N, Antunes K, et al. Comparison of two sequential extraction procedures for uranium fractionation in contaminated soils[J]. Journal of Environmental Radioactivity, 2014, 137:1-9.
Acosta-Santoyo G, Cameselle C, Bustos E, et al. Electrokinetic-enhanced ryegrass cultures in soils polluted with organic and inorganic compounds[J]. Environmental Research, 2017,158:118-125.
Cang L, Wang Q, Zhou D, et al. Effects of electrokinetic-assisted phytoremediation of a multiple-metal contaminated soil on soil metal bioavailability and uptake by Indian mustard[J]. Separation and Purification Technology, 2011,79(2):246-253.
周生学.直流电刺激对细菌生长动态过程的作用研究[D]. 北京:首都师范大学, 2009. Zhou S X. Effects of direct current stimulation on the dynamic process of bacterial growth[D]. Beijing:Capital Normal University, 2009.
陈珍芳.海马齿对镉的耐受与解毒机制研究[D]. 厦门:厦门大学, 2017. Chen G F. Study on tolerance and detoxification mechanism of hippocampal teeth to cadmium[D]. Xiamen:Xiamen University, 2017.
Probstein R F, Hicks R E. Removal of contaminants from soils by electric fields[J]. Science, 1993,260:498-503.
Sanz M, López-Arias M, Rebollar E, et al. Influence of electrical fields (AC and DC) on phytoremediation of metal polluted soils with rapeseed (Brassica napus) and tobacco (Nicotiana tabacum)[J]. Chemosphere, 2011,83(3):318-326.
Vanhoudt N, Vandenhove H, Horemans N, et al. Study of oxidative stress related responses induced in Arabidopsis thaliana following mixed exposure to uranium and cadmium[J]. Plant Physiology and Biochemistry, 2010,48(10/11):879-886.
Horemans N, Van Hees M, Van Hoeck A, et al. Uranium and cadmium provoke different oxidative stress responses in Lemna minor L.[J]. Plant Biology, 2014,17:91-100.
李仕友,熊凡,欧阳成炜,等.万年青在镉铀胁迫下的富集特征和生理生化机制[J]. 安全与环境学报, 2017,17(6):394-399. Li S Y, Xiong F, Ouyang C W, et al. Enrichment characteristics and physiological and biochemical mechanism of evergreen under cadmium and uranium stress[J]. Chinese Journal of Safety and Environment, 2017,17(6):394-399.
Hanssan A, Ran B, Michael S, et al. Electrokinetic enhancement on phytoremediation in Zn, Pb, Cu and Cd contaminated soil using potato plants[J]. Journal of Environmental Science and Health Part A, 2008,43(8):926-933.
胡宏韬,程金平.土壤铜镉污染的电动力学修复实验[J]. 生态环境学报, 2009,18(2):511-514. Hu H T, Cheng J P. Electrodynamic remediation of soil copper and cadmium pollution[J]. Journal of Ecology and Environment, 2009,18(2):511-514.
徐卫红,黄河,王爱华,等.根系分泌物对土壤重金属活化及其机理研究进展[J]. 生态环境学报, 2006,15(1):184-189. Xu W H, Huang H, Wang A H, et al. Research progress on the activation of soil heavy metals by root exudates and its mechanism[J]. Chinese Journal of Ecology and Environment, 2006,15(1):184-189.
Shahandeh H, Hossner L R. Enhancement of uranium phytoaccumulation from contaminated soils[J]. Soil Science, 2002, 167(4):269-280.
Soudek P, Petrová Š, Vaněk T. Heavy metal uptake and stress responses of hydroponically cultivated garlic (Allium sativum L.)[J]. Environmental and Experimental Botany, 2011,74:289-295.
Qin R, Hirano Y, Brunner I. Exudation of organic acid anions from poplar roots after exposure to Al, Cu and Zn[J]. Tree Physiology, 2007,27(2):313-320.
Evangelou M, Ebel M, Schaeffer A, et al. Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity, and fate of chelating agents[J]. Chemosphere, 2007,68(6):0-1003.
Nawaz M A, Jiao Y, Chen C, et al. Melatonin pretreatment improves vanadium stress tolerance of watermelon seedlings by reducing vanadium concentration in the leaves and regulating melatonin biosynthesis and antioxidant-related gene expression[J]. Journal of Plant Physiology, 2018,220:115-127.
Wang L, Zhang X, Zhou Q, et al. Effects of terbium (III) on signaling molecules in horseradish[J]. Biological Trace Element Research, 2015,164(1):122-129.
王淑妍,郭九峰,刘晓婷,等.静电场的植物生物效应研究概述[J]. 中国农学通报, 2015,31(35):73-78. Wang S Y, Guo J F, Liu X T, et al. Overview of plant biological effects of electrostatic field[J]. Chinese Agricultural Science Bulletin, 2015,31(35):73-78.
Li J, Zhang J, Larson S L, et al. Electrokinetic-enhanced phytoremediation of uranium contaminated soil using sunflower and Indian mustard[J]. International Journal of Phytoremediation, 2019, 21(12):1197-1204.
Cameselle C, Gouveia S, Urréjola S. Benefits of phytoremediation amended with DC electric field. Application to soils contaminated with heavy metals[J]. Chemosphere, 2019,229:481-488.
Cameselle C, Gouveia S. Phytoremediation of mixed contaminated soil enhanced with electric current[J]. Journal of Hazardous Materials, 2019,361:95-102.
雷鸣,廖柏寒,秦普丰.土壤重金属化学形态的生物可利用性评价[J]. 生态环境学报, 2007,16(5):1551-1556. Lei M, liao B H, Qin P F. Bioavailability evaluation of heavy metal chemical morphology in soil[J]. Journal of ecological environment, 2007,16(5):1551-1556.
Vanhoudt N, Vandenhove H, Horemans N, et al. Study of oxidative stress related responses induced in Arabidopsis thaliana following mixed exposure to uranium and cadmium[J]. Plant Physiology & Biochemistry, 2010,48(10):879-886.
Zou C, Sha Y H, Ding D X, et al. Aspergillus niger changes the chemical form of uranium to decrease its biotoxicity, restricts its movement in plant and increase the growth of Syngonium podophyllum[J]. Chemosphere, 2019,224:316-323.
He J D, Wang Y D, Hu N, et al. An artificially constructed Syngonium podophyllum-Aspergillus niger combinate system for removal of uranium from wastewater[J]. Environmental Science and Pollution Research, 2015,22:18918-18926.
Broeckling C D, Broz A K, Bergelson J, et al. Root exudates regulate soil fungal community composition and diversity[J]. Applied and Environmental Microbiolgy, 2008,74(3):738-744.
Ameloot N, Neve D, Jegajeevagan K, et al. Short-term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils[J]. Soil Biology and Biochemistry, 2013,57:401-410.
Barns S M, Cain E C, Sommerville L, et al. Acidobacteria phylum sequences in uranium-contaminated subsurface sediments greatly expand the known diversity within the phylum[J]. Applied and Environmental Microbiology, 2007,73(9):3113-3116.
Lyons J I, Newell S Y, Buchan A, et al. Diversity of ascomycete laccase gene sequences in a southeastern US salt marsh[J]. Microbial Ecology, 2003,45(5):270-281.
Jiang S, Wang W, Xue X, et al. Fungal diversity in major oil-shale mines in China[J]. Journal of Environmental Sciences (China), 2016, 41:81-89.
Wang Y D, Li G Y, Deng Q W, et al. Uranium leaching using mixed organic acids produced by Aspergillus niger [J]. Journal of Radioanalytical and Nuclear Chemistry, 2013,298(2):769-773.