Abstract：In this work, the binding heterogeneities and mechanism of Fe(III) with digestate dissolved organic matter (DOM) derived from anaerobic fermentation of cow manure was investigated through fluorescence spectra, Fourier Transform Infrared spectroscopy (FTIR), parallel factor analysis (PARAFAC) and two-dimensional correlation spectroscopy (2D-COS). The results showed that six fluorescent components, including protein-like (C2), fulvic-like (C1), humic-like (C4 and C5), a combination of protein-like and fulvic-like (C3), a combination of protein-like and humic-like (C6) could be identified by PARAFAC model. However, only protein-like and fulvic-like components could be identified by 2D-SYS-COS, and protein-like were dominant fluorescent component in DOM. 2D-COS analysis revealed that fulvic-like substances showed a preferential affinity with Fe(III) at 334nm, and the preferential bonding followed the order:334nm→306nm. The −NH2 group of secondary ammonium could preferentially combine with Fe(III), and the preferential binding of Fe(III) to the digestate DOM followed the order:2265→2771→1528→1310→1805→ 1479cm-1. The results of double log equation indicated that highly chemically stable DOM-Fe(III) complexes could be formed, whose logarithmic conditional stability ranged from 4.34 to 7.03. The work herein could provide a theoretical guide for the species distribution, migration and transformation of metal ions when digestate was applied to soils.
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