Environmental interpretation of spatial variation of planktonic bacterial communities in the North Canal River
JIN Yan1, QIU Ying1, DONG Zhi2, DI Yan-ming3, ZHAO Dong-liang1, GUO Xiao-yu1
1. College of Resources Environment and Tourism, Capital Normal University, Beijing 100048, China; 2. School of Life Sciences, Peking University, Beijing 100871, China; 3. Beijing North Canal Manage Office, Beijing 101100, China
Abstract：The contribution of environmental selection to the construction mechanism of phytoplankton diversity in fine geographical scale rivers has been widely recognized. According to ecological theory of metacommunity, the determinant factors between the Core Operational Taxonomic Units (core OTUs) and the Unique Operational Taxonomic Units (unique OTUs) are potential to be different, as these two key components of microbial community in river ecosystems vary in dispersal ability and biological responses. To test this hypothesis, the Beijing-Tianjin-Hebei section of the Northern Canal River, which is highly artificial, was selected as the study area. To interpret the spatial variation of the core OTUs in local-community and the unique OTUs, the water quality monitoring results and high-throughput sequencing results were combined for comprehensive analysis. The results showed that inorganic nitrogen, cation such as Ca2+, Mg2+ induced by reclaimed water recharge and organic pollution caused by physical disturbance of reclaimed water recharge port were the key pollutants in the North Canal River. The intra-group similarity of the core OTUs in local-community was significantly higher than that of the unique OTUs, and the geographical distribution pattern and environmental divers differed a lot between these two key components. The spatial distribution law of core OTUs in local community was significantly consistent with geographic spatial distribution pattern, and environmental variables most closely related to spatial changes were TN, nitrate nitrogen (NO3--N), K+, Ca2+, Na+ and Mg2+. Specifically, these critical environmental variables showed a significant decrease trend with the direction of water flow and showed a strong synergistic change with the geographical diffusion distance. While, unique OTUs in local-communities did not have a significant geographic spatial distribution pattern, and the crucial environmental variables determining the spatial distribution of the unique OTUs in local-communities were temperature, oxidation-reduction potential and pH, which were caused by the indirect effects of physical disturbance on hydrological quality of reclaimed water supply.
Newton R J, Jones S E, Eiler A, et al. A guide to the natural history of freshwater lake bacteria[J]. Microbiol Mol Biol R, 2011,75(1):14-49.
Eva S. Lindstrom A E, Silke Langenheder S B S D, Henrik Ragnarsson A L J T. Does ecosystem size determine aquatic bacterial richness? Comment[J]. Ecology, 2007,1(88):252-253.
Yannarell A C, Triplett E W. Geographic and environmental sources of variation in lake bacterial community composition[J]. Appl Environ Microb, 2005,1(71):227-239.
Whitaker R J, Grogan D W, Taylor J W. Geographic barriers isolate endemic populations of Hyperthermophilic Archaea[J]. Science, 2003,301(5635):976-978.
Papke R T, Ramsing N B, Bateson M M, et al. Geographical isolation in hot spring cyanobacteria[J]. Environ Microbiol, 2003,5(8):650-659.
Cottenie K. Integrating environmental and spatial processes in ecological community dynamics[J]. Ecol Lett, 2005,8(11):1175-1182.
Xiong W, Ni P, Chen Y, et al. Zooplankton community structure along a pollution gradient at fine geographical scales in river ecosystems:The importance of species sorting over dispersal[J]. Mol Ecol, 2017,26(16):4351-4360.
于洋,王晓燕,张鹏飞.北运河水体浮游细菌群落的空间分布特征及其与水质的关系[J]. 生态毒理学报, 2012,7(3):337-344. Yang Y, Xiaoyan W, Pengfei Z. Spatial distribution of planktonic bacterial community and its relationship to water quality in Beiyun River[J]. Asian Journal of Ecotoxicolog, 2012,7(3):337-344.
Chase J M, Myers J A. Disentangling the importance of ecological niches from stochastic processes across scales[J]. Philosophical Transactions of the Royal Society B:Biological Sciences, 2011, 366(1576):2351-2363.
Chase J M. Stochastic community assembly causes higher biodiversity in moreProductive environments[J]. Science, 2010,328(5984):1388-1391.
Liu L, Yang J, Yu Z, et al. The biogeography of abundant and rare bacterioplankton in the lakes and reservoirs of China[J]. The ISME journal, 2015,9(9):2068-2077.
Pandit S N, Kolasa J, Cottenie K. Contrasts between habitat generalists and specialists:An empirical extension to the basic metacommunity framework[J]. Ecology, 2009,90(8):2253-2262.
Kolasa J, Li B. Removing the confounding effect of habitat specialization reveals the stabilizing contribution of diversity to species variability[J]. Proceedings of the Royal Society of London. Series B:Biological Sciences, 2003,270(suppl):S198-S201.
Barberan A, Bates S T, Casamayor E O, et al. Using network analysis to explore co-occurrence patterns in soil microbial communities[J]. Isme J, 2012,6(2):343-351.
Sriswasdi S, Yang C, Iwasaki W. Generalist species drive microbial dispersion and evolution[J]. Nat Commun, 2017,8(1).
Graham E B, Stegen J C. Dispersal-based microbial community assembly decreases biogeochemical function[J]. Processes, 2017, 5(4):65.
Chen J, Wang P, Wang C, et al. Distinct assembly mechanisms underlie similar biogeographic patterns of rare and abundant bacterioplankton in cascade reservoirs of a large river[J]. Front Microbiol, 2020,11:158.
Cao X, Zhao D, Zeng J, et al. Biogeographic patterns of abundant and rare bacterial and microeukaryotic subcommunities in connected freshwater lake zones subjected to different levels of nutrient loading[J]. J Appl Microbiol, 2020.
李彦东,李红有.对北运河治理规划的思考[J]. 海河水利, 2006, (1):24-27. Li Y, Li H. Thinking of river conservancy plan of BeiyunRiver[J]. Haihe Water Resources, 2006.
Yang Y, Gao Y, Huang X, et al. Adaptive shifts of bacterioplankton communities in response to nitrogen enrichment in a highly polluted river[J]. Environ Pollut, 2019,245:290-299.
刘宇同,杨伟超,杨丽娜,等.北运河流域水生态恢复与保护的实践探索[J]. 北京水务, 2019,(3):57-62. Liu Y, Yang W, Yang L, et al. Practice and exploration of water ecology construction and protection in the North Canal river basin[J]. Beijing Water, 2019:57-62.
Malard L A, Anwar M Z, Jacobsen C S, et al. Biogeographical patterns in soil bacterial communities across the Arctic region[J]. Fems Microbiol Ecol, 2019,95(9).
李海云,邸琰茗,李东青,等.北京市潮白河再生水补水河段水质时空变异[J]. 环境科学研究, 2017,30(10):1542-1552. Li H, Di Y, Li D, et al. Spatial and temporal variations of water quality in a wetland-reclaimed water-supplied purification urban river:case study in Chaobai River of Beijing[J]. Research of Environmental Sciences, 2017,30(10).
郭婧,荆红卫,李金香,等.北运河系地表水近10年来水质变化及影响因素分析[J]. 环境科学, 2012,33(5):1511-1518. Guo J, Jing H, Li J, et al. Surface water quality of Beiyun Rivers Basin and the analysis of acting factors for the recent ten years[J]. Chinese Journal of Environmental Science, 2012,33(5).
何腾,熊家晴,王晓昌,等.不同再生水补水比例下景观水体的水质变化[J]. 环境工程学报, 2016,10(12):6923-6927. Teng H E, Jiaqing X, Xiaochang W, et al. Quality variations of landscape water with different ratio of reclaimed water supply[J]. Chinese Journal of Environmental Engineering, 2016,10(12).
黄迪,熊薇,刘克,等.典型再生水人工湿地净化系统水质时空变异研究——以北京市奥林匹克森林公园人工湿地为例[J]. 环境科学学报, 2014,34(7):1738-1750. Huang D, Xiong W, Liu K, et al. Temporal-spatial variations of water quality in a reclaimed-water-supplied constructed wetland purification system:A case study in Olympic Forest Park of Beijing[J]. Acta Scientiae Circumstantiae, 2014,34(7).
鲍林林,陈永娟,王晓燕.北运河沉积物中氨氧化微生物的群落特征[J]. 中国环境科学, 2015,35(1):179-189. Bao L, Chen Y, Wang X. Diversity and abundance of ammonia-oxidizing prokaryotes in surface sediments in Beiyun River[J]. China Environmental Science, 2015,35(1).
张晓娇.北运河底泥污染特征及内源污染控制技术研究[D]. 大连:大连海洋大学生态学, 2018. Zhang X. The characteristics of pollutants in sediment and the research of in-situ controlling in Beiyun River, China[D]. Dalian:Dalian Ocean University生态学, 2018.
逄勇,颜润润,余钟波,等.风浪作用下的底泥悬浮沉降及内源释放量研究[J]. 环境科学, 2008,(9):2456-2464. Pang Y, Yan R, Yu Z, et al. Suspension-sedimentation of sediment and release amount of internal load in lake Taihu affected by wind[J]. Environmental Science, 2008,(9).
曾巾,杨柳燕,肖琳,等.太湖不同湖区无机氮转化潜力[J]. 生态与农村环境学报, 2008,(1):63-67. Ceng J, Yang L, Xiao L, et al. Comparative study on transformation potential of dissolved inorganic nitrogen in different parts of Lake Taihu[J]. Journal of Ecology and Rural Environment, 2008,(1).
吴雅丽,许海,杨桂军,等.太湖水体氮素污染状况研究进展[J]. 湖泊科学, 2014,26(1):19-28. Yali W U, Hai X U, Guijun Y, et al. Progress in nitrogen pollution research in Lake Taihu[J]. Journal of Lake Sciences, 2014,26(1).
王玮.水平潜流人工湿地强化脱氮的技术及其机制研究[D]. 上海:东华大学, 2017. Wang W. Technique and mechanism of intensified nitrogen removal in horizontal subsurface flow constructed wetland[D]. Shanghai:Donghua University, 2017.
蔚枝沁.水生植被恢复对城市水体沉积物磷素形态与释放的影响[D]. 上海:华东师范大学, 2012. Yu Z. Effects of aquatic re-vegetation on phosphorus fractions in and release from the sediments in urban rivers[D]. Shanghai:East China Normal University, 2012.