Study on the distribution and removal effect of resistance genes in integrated system of A2/O-MBR
LI Zhong-hong1,2, DU Cai-li2,3, CHEN Su-hua1, ZHANG Lie-yu2, LI Xiao-guang2, LI Jia-xi2, TIAN Zhen-jun2
1. Key Laboratory of Jiangxi Province for Persistant Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang 330063, China; 2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; 3. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
Abstract：The increase of antibiotic resistance genes among microorganisms has become the main transmission source for sewage treatment plants. The purpose of this study was to explore the removal effect of Antibiotic Resistance Genes (ARGs) and pathogenic bacteria in rural domestic sewage treatment process and evaluate the water quality safety. This study selected a integration A2/O-MBR wastewater treatment system in the village of Beijing, systematic study was implemented in integrated system of A2/O-MBR wastewater treatment system to get the distribution law of ARGs and pathogenic bacteria in the each processing unit. Based on macro genomics, the high-throughput sequencing technology was used to analysis the the ability of removing ARGs and pathogenic bacteria in genus through integrated system of A2/O-MBR. Results showed that the ARGs widely existed in sewage treatment system, 19kinds of ARGs including tetracycline class, aminoglycoside class and sulfonamide class were detected, the relative abundance of the ARGs in influent was much higher than its concentration in the effluent, relative abundance of the ARGs fell by 72.25%, but most of the ARGs in sewage treatment system was not fully removed. The changes of microbial community structure showed that the relative abundance of 32potential pathogens decreased significantly, and most pathogenic bacteria could not be completely removed. The residual ARGs and pathogenic bacteria in the water will lead to the potential pollution risk of receiving water body.
李中浤, 杜彩丽, 陈素华, 张列宇, 李晓光, 黎佳茜, 田振军. 一体化A2/O-MBR系统中抗性基因分布及去除效果研究[J]. 中国环境科学, 2021, 41(9): 4135-4141.
LI Zhong-hong, DU Cai-li, CHEN Su-hua, ZHANG Lie-yu, LI Xiao-guang, LI Jia-xi, TIAN Zhen-jun. Study on the distribution and removal effect of resistance genes in integrated system of A2/O-MBR. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(9): 4135-4141.
Sanderson H, Fricker C, Brown R S, et al. Antibiotic resistance genes as an emerging environmental contaminant[J]. Dossiers Environnement, 2016,24(2):205-218.
[2]
Frost L S, Leplae R, Summers A O, et al. Mobile genetic elements:the agents of open source evolution[J]. Nature Reviews Microbiology, 2005,3(9):722-732.
[3]
Zhang H, He H, Chen S, et al. Abundance of antibiotic resistance genes and their association with bacterial communities in activated sludge of wastewater treatment plants:Geographical distribution and network analysis[J]. Journal of Environmental Sciences, 2019,82(8):24-38.
[4]
Martínez J L. Antibiotics and antibiotic resistance genes in natural environments[J]. Science, 2008,321(5887):365-367.
[5]
Wang Z, Han M, Li E, et al. Distribution of antibiotic resistance genes in an agriculturally disturbed lake in China:Their links with microbial communities, antibiotics, and water quality[J]. Journal of Hazardous Materials, 2020,393:122426.
[6]
Sun J, Jin L, He T, et al. Antibiotic resistance genes (ARGs) in agricultural soils from the Yangtze River Delta, China[J]. Science of The Total Environment, 2020,740:140001.
[7]
Zhang Y, Hu H, Yan H, et al. Salinity as a predominant factor modulating the distribution patterns of antibiotic resistance genes in ocean and river beach soils[J]. Science of The Total Environment, 2019,668:193-203.
[8]
Guo J, Li J, Chen H, et al. Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements[J]. Water Research, 2017,123:468-478.
[9]
Ju F, Li B, Ma L, et al. Antibiotic resistance genes and human bacterial pathogens:Co-occurrence, removal, and enrichment in municipal sewage sludge digesters[J]. Water Research, 2016,91:1-10.
[10]
Manaia C M, Rocha J, Scaccia N, et al. Antibiotic resistance in wastewater treatment plants:Tackling the black box[J]. Environment International, 2018,115:312-324.
[11]
Lapara T M, Burch T R, Mcnamara P J, et al. Tertiary-treated municipal wastewater is a significant point source of antibiotic resistance genes into duluth-superior harbor[J]. Environmental Science & Technology, 2011,45(22):9543-9549.
[12]
Mao D, Yu S, Rysz M, et al. Prevalence and proliferation of antibiotic resistance genes in two municipal wastewater treatment plants[J]. Water Research, 2015,85:458-466.
[13]
马奔,黄雅梦,王若楠,等.城市污水厂MCR-1基因及其携带菌的污染[J]. 中国环境科学, 2018,38(4):235-242.Ma B, Huang Y M, Wang R N, et al. The pollution of MCR-1and MCR-1hosting bacteria in municipal wastewater treatment plants[J]. China Environmental Science, 2018,38(4):235-242.
[14]
Chen H, Zhang M. Occurrence and removal of antibiotic resistance genes in municipal wastewater and rural domestic sewage treatment systems in eastern China[J]. Environment International, 2013,55:9-14.
[15]
Chen J, Liu Y S, Su H C, et al. Removal of antibiotics and antibiotic resistance genes in rural wastewater by an integrated constructed wetland[J]. Environmental Science & Pollution Research International, 2015,22(3):1794-1803.
[16]
Szczepanowski R, Linke B, Krahn I, et al. Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics[J]. Microbiology, 2009,155(7):2306-2319.
[17]
Bolger A M, Lohse M, Usadel B. Trimmomatic:a flexible trimmer for Illumina sequence data[J]. Bioinformatics, 2014,30(15):2114-2120.
[18]
Wood D E, Lu J, Langmead B. Improved metagenomic analysis with Kraken 2[J]. Genome biology, 2019,20(1):257.
[19]
Yin X, Jiang X, Chai B, et al. ARGs-OAP v2.0 with an expanded SARG database and Hidden Markov Models for enhancement characterization and quantification of antibiotic resistance genes in environmental metagenomes[J]. Bioinformatics, 2018,34(13):2263-2270.
[20]
Li B, Yang Y, Ma L, et al. Metagenomic and network analysis reveal wide distribution and co-occurrence of environmental antibiotic resistance genes[J]. The ISME Journal, 2015,9(11):2490-2502.
[21]
Albertsen M, Hugenholtz P, Skarshewski A, et al. Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes[J]. Nature Biotechnology, 2013,31(6):533-538.
[22]
Tang J Y, Bu Y Q, Zhang X X, et al. Metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water[J]. Ecotoxicology & Environmental Safety, 2016,132:260-269.
[23]
Yang Y, Li B, Zou S, et al. Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach[J]. Water Research, 2014,62:97-106.
[24]
Zhang T, Yang Y, Pruden A. Effect of temperature on removal of antibiotic resistance genes by anaerobic digestion of activated sludge revealed by metagenomic approach[J]. Applied Microbiology & Biotechnology, 2015,99(18):7771-7779.
[25]
Yoo K, Yoo H, Lee J, et al. Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis[J]. Journal of Microbiology, 2020,58(2):123-130.
[26]
Du J, Geng J, Ren H, et al. Variation of antibiotic resistance genes in municipal wastewater treatment plant with A 2O-MBR system[J]. Environmental Science and Pollution Research, 2015,22(5):3715-3726.
[27]
Lekunberri, Itziar, Rafraf, et al. Abundance of antibiotic resistance genes in five municipal wastewater treatment plants in the Monastir Governorate, Tunisia[J]. Environmental Pollution, 2016,219:353-358.
[28]
Figueira V, Serra E, Manaia C M. Differential patterns of antimicrobial resistance in population subsets of Escherichia coli isolated from waste-and surface waters[J]. Science of the Total Environment, 2011, 409(6):1017-1023.
[29]
Wang Z, Zhang X X, Huang K, et al. Metagenomic profiling of antibiotic resistance genes and mobile genetic elements in a tannery wastewater treatment plant[J]. Plos One, 2013,8(10):e76079.
[30]
Huovinen P, Sundström L, Swedberg G, et al. Trimethoprim and sulfonamide resistance.[J]. Antimicrobial agents and chemotherapy, 1995,39(2):279-289.
[31]
Akiba T, Koyama K, Ishiki Y, et al. On the mechanism of the development of multiple drug-resistant clones of Shigella[J]. Jpn J Microbiol, 1960,4(2):219-227.
[32]
Lamba M, Graham D W, Ahammad S Z. Hospital wastewater releases of carbapenem-resistance pathogens and genes in urban India[J]. Environmental Science & Technology, 2017,51(23):13906-13912.
[33]
Heuer H, Binh C T T, Jechalke S, et al. IncP-1ε plasmids are important vectors of antibiotic resistance genes in agricultural systems:diversification driven by class 1integron gene cassettes[J]. Frontiers in microbiology, 2012,3:2.
[34]
Clark N C, Olsvik Ø, Swenson J M, et al. Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in enterococcus faecalis[J]. Antimicrobial Agents & Chemotherapy, 1999,43(1):157-160.
[35]
Tong J, Lu X, Zhang J, et al. Factors influencing the fate of antibiotic resistance genes during thermochemical pretreatment and anaerobic digestion of pharmaceutical waste sludge[J]. Environmental Pollution, 2018,243(PT.B):1403-1413.
[36]
孔晓,崔丙健,金德才,等.农村污水膜生物反应器系统中微生物群落解析[J]. 环境科学, 2015,36(9):3329-3338.Kong X, Cui B J, Jin D C, et al. Analysis of microbial community in the membrane bio-reactor(MBR) rural sewage treatment system[J]. Environmental Science, 2015,36(9):3329-3338.
[37]
Jian Y, Lihong C, Lilian S, et al. VFDB 2008 release:an enhanced web-based resource for comparative pathogenomics[J]. Nucleic Acids Research, 2008,36:539-542.
[38]
Forsberg K J, Reyes A, Wang B, et al. The shared antibiotic resistome of soil bacteria and human pathogens[J]. 2012,337(6098):1107-1111.
[39]
罗晓,袁立霞,张文丽,等.制药废水厂抗性基因和微生物群落相关性研究[J]. 中国环境科学, 2019,39(2):831-838.Luo X, Yuan L X, Zhang W L, et al. Correlation study between resistance genes and microbial communities in pharmaceutical wastewater treatment plants[J]. China Environmental Science, 2019, 39(2):831-838.
