Dynamics of eARGs in activated sludge in response to starvation at different temperatures as revealed by metagenomics
TANG Zhen-ping1,2, LIU Bo-yang3, GAO Yuan-yuan2, DUAN Yi1,3, YANG Zheng-qing3, ZHOU Shuai1,2,3
1. Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang 421001, China; 2. Hunan Province Key Laboratory of Rare Metal Mineral Exploitation and Geological Disposal of Wastes, University of South China, Hengyang 421001, China; 3. School of Civil Engineering, University of South China, Hengyang 421001, China
Abstract：Activated sludge is a vital reservoir for extracellular antibiotic resistance genes (eARGs). The metagenomic sequencing technology was used for the first time to examine the impacts of starvation (i.e., substrate deficiency) at different temperatures (12℃, 20℃ and 30℃) on the dynamic of eARGs in activated sludge. The results indicated that the abundance of eARGs after 30-days starvation was effectively reduced by 3.5%~26.0%, 66.1%~81.4%, respectively. The abundance of eARGs after 12℃ starvation was higher than that after 20℃ and 30℃ starvation. The majority of extracellular mobile genetic elements changed little after starvation at 12℃, but their abundance after 12℃ and 30℃ starvation decreased by 41.4%~54.4%, 48.7%~67.4%, respectively. The relative abundance of the dominated hosts of eARGs (i.e., Mycobacterium and Nitrospira) remained stable after starvation at different temperatures. The kinetic studies confirmed that, compared with 20℃ and 30℃ starvation, 12℃ starvation had higher proportion of living cells and relatively lower adsorption or degradation rate of extracellular DNA, which was conducive to maintain the stability of eARGs. The present study was helpful for elucidating the fate of eARGs in activated sludge in response to starvation stress at different temperatures, which could provide a scientific basis for controlling the propagation and dissemination of eARGs in actual wastewater treatment plants.
唐振平, 刘博阳, 高媛媛, 段毅, 杨正卿, 周帅. 宏基因组解析不同温度饥饿胁迫下污泥中胞外耐药基因的动态特征[J]. 中国环境科学, 2023, 43(3): 1397-1406.
TANG Zhen-ping, LIU Bo-yang, GAO Yuan-yuan, DUAN Yi, YANG Zheng-qing, ZHOU Shuai. Dynamics of eARGs in activated sludge in response to starvation at different temperatures as revealed by metagenomics. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(3): 1397-1406.
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