Impact of different pretreatments on ARGs and live microbial communities in excess sludge
MAO Qiu-yan1, ZHAO Xu-ning1, SU Yu-ao1, ZHANG Hui-min1, LIU He1,2,3, FU Bo1,2,3, ZHANG Yan1,2,3
1. School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China;
2. Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China;
3. Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215009, China
Pretreatment can not only facilitate the subsequent anaerobic digestion (AD) of excess sludge, but also may decrease the abundance of antibiotic resistance genes (ARGs) and reduce the risk of ARGs spreading during AD. In this study, the impact of different pretreatments on ARGs and live microbial communities in excess sludge was investigated based on propidium monoazide (PMA) treatment. The results showed that the removal of ARGs by thermo-alkaline, thermal hydrolysis, and microwave pretreatments were 3.32logs, 3.13logs, and 2.95logs, respectviely. The ultrasonic wave pretreatment only removed 0.58logs of the ARGs. With the time extension of thermal hydrolysis, the microorganisms was dramatically removed during 1~2hours. The removal of ARGs was 2.42 logs with thermal hydrolysis extended to 4 hours. Proteobacteria was the dominant bacteria in the raw excess sludge. With the pretreatment of microwave and thermal hydrolysis, Firmicutes and Chloroflexi was greatly improved. The correlation analysis showed that sulI and tetC was very similar that significant positive correlated with several families of microorganisms, which indicated various kinds of the potential host bacteria. However, the good removal of sulI and tetC during pretreatment indicated the poor tolerance of their hosts to the pretreatments. No significant positive correlation was found between ermB and microbial communities, and only few families correlated with tetA. Most ARGs carrying bacteria have little resistance with thermo-alkaline, thermal hydrolysis, and microwave pretreatments, and it is necessary to extend the pretreatment time of thermal hydrolysis appropriately to effectively reduce the ARGs in sludge.
毛秋燕, 赵栩宁, 苏宇傲, 张慧旻, 刘和, 符波, 张衍. 不同预处理方式对剩余污泥中活菌菌群及ARGs的影响[J]. 中国环境科学, 2020, 40(6): 2537-2545.
MAO Qiu-yan, ZHAO Xu-ning, SU Yu-ao, ZHANG Hui-min, LIU He, FU Bo, ZHANG Yan. Impact of different pretreatments on ARGs and live microbial communities in excess sludge. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2537-2545.
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