Partial nitrification-anaerobic ammonia oxidation for the treatment of moderately concentrated ammonia-nitrogen wastewater: Effect of intermittent aeration on nitrogen removal performance
ZHOU Meng-yu, PENG Dang-cong, HAN Yun, Lü Kai
Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：A one-stage PN/A(partial nitrification coupled with anaerobic ammonia oxidation) process was successfully implemented using a lab-scale MBBR reactor with the intermittent aeration. The total nitrogen removal efficiency reached 83.74% under the condition of 35℃, 150.00 mg/L influent ammonia nitrogen, 0.24 kg/(m3·d) influent nitrogen loading rate and(1.41±0.24)mg/L DO concentration. The maximum activity of anaerobic ammonia oxidizing bacteria(AnAOB) and ammonia oxidizing bacteria(AOB) in biofilms were 3792.00 and 5166.00 mg/(m2·d), respectively, while nitrite oxidizing bacteria(NOB) activity was not detected. The results of high throughput analysis showed that the AnAOB was mainly Candidatus Brocadia with a relative abundance of 1.33%,and the dominated AOB and NOB were Nitrosomonas at 0.17% relative abundance and Nitrospira at 0.003% relative abundance,respectively. The efficient PN/A process could be achieved in MBBR reactors at moderate ammonia-nitrogen concentration, and the augmentation of DO concentrations by the intermittent aeration could effectively improve the nitrogen removal performance.
Nsenga Kumwimba M, Lotti T, Senel E, et al. Anammox-based processes:How far have we come and what work remains?A review by bibliometric analysis[J]. Chemosphere, 2020,238:124627.
Wett B. Development and implementation of a robust deammonification process[J]. Water Science and Technology, 2007,56(7):81-88.
Kartal B, Kuenen J G, van Loosdrecht M C M. Sewage treatment with anammox[J]. Science, 2010,328(5979):702-703.
Cao Y S, van Loosdrecht M C M, Daigger G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status,bottlenecks, and further studies[J]. Applied Microbiology Biotechnology, 2017,101:1365-1383.
李冬,赵世勋,王俊安,等.污水处理厂CANON工艺启动策略[J].中国环境科学, 2017,37(11):4125-4131.Li D, Zhao S X, Wang J A, et al. Startup strategies of CANON process in wastewater treatment plant[J]. China Environmental Science,2017,37(11):4125-4131.
Strous M, Kuenen J G, Jetten M S. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology,1999,65(7):3248-3250.
Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences-An application survey[J]. Water Research, 2014,55:292-303.
Chen R, Ji J Y, Chen Y J, et al. Successful operation performance and syntrophic micro-granule in partial nitritation and anammox reactor treating low-strength ammonia wastewater[J]. Water Research,2019,155:288-299.
狄斐,隋倩雯,陈彦霖,等.部分亚硝化-厌氧氨氧化处理磁混凝生活污水[J].中国环境科学, 2020,40(11):4712-4720.Di F, Sui Q W, Chen Y L, et al. Partial nitritation-Anammox process treating magnetic coagulation domestic sewage[J]. China Environmental Science, 2020,40(11):4712-4720.
赵良杰,王静,彭党聪,等.一段式部分亚硝化-厌氧氨氧化工艺处理中低浓度模拟氨氮废水[J].环境工程学报, 2021,15(1):143-151.Zhao L J, Wang J, Peng D C, et al. Treatment of simulated medium and low-strength ammonia wastewater by single-stage partial nitritation-anammox process[J]. Chinese Journal of Environmental Engineering, 2021,15(1):143-151.
van de Graaf A A, de Bruijn P, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142(8):2187-2196.
于莉芳,陈青青,杨晋,等.污泥水富集硝化菌的群落结构及动力学参数研究[J].环境科学, 2009,30(7):2035-2039.Yu L F, Chen Q Q, Yang J, et al. Community structure and kinetics characterization of enriched nitrifiers cultivated with reject water[J].Environmental Science, 2009,30(7):2035-2039.
国家环境保护总局.水和废水监测分析方法(第四版)[M].第4版.北京:中国环境科学出版社, 2002:200-284.State Environmental Protection Administration of China. Water and waste water monitoring and analysis method[M]. 4th Edition. Beijing.China Environmental Science Press, 2002:200-284.
Lotti T, Kleerebezem R, Lubello C, et al. Physiological and kinetic characterization of a suspended cell anammox culture[J]. Water Research, 2014,60:1-14.
Anthonisen A C, Loehr R C, Prakasam T B S, et al. Inhibition of nitrification by ammonia and nitrous acid.[J]. Water Pollution Control Federation, 1976,48(5):835-852.
Cruz Bournazou M N, Hooshiar K, Arellano-Garcia H, et al. Model based optimization of the intermittent aeration profile for SBRs under partial nitrification[J]. Water Research, 2013,47(10):3399-3410.
Cao S B, Du R, Li B K, et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J]. Applied Microbiology and Biotechnology, 2016,100(14):6457-6467.
Kuenen J G. Anammox and beyond[J]. Environmental Microbiology,2020,22(2):525-536.
Yang Y D, Zhang L, Chen J, et al. Microbial community evolution in partial nitritation/anammox process:From sidestream to mainstream[J]. Bioresource Technology, 2018,251:327-333.
Huang T, Zhao J Q, Wang S, et al. Fast start-up and enhancement of partial nitritation and anammox process for treating synthetic wastewater in a sequencing bath biofilm reactor:Strategy and function of nitric oxide[J]. Bioresource Technology, 2021,335:125225.
吕恺,邵贤明,王康舟,等.一段式亚硝化厌氧氨氧化SMBBR处理中低浓度氨氮废水[J].环境科学, 2021,42(7):3385-3391.LUV K, Shao X M, Wang K Z, et al. Treatment of medium ammonium wastewater by single-stage partial nitritationANAMMOX SMBBR[J]. Environmental Science, 2021,42(7):3385-3391.
Regmi P, Miller M W, Holgate B,et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research, 2014,57:162-171.
Liu T, Li D, Zhang J, et al. Effect of temperature on functional bacterial abundance and community structure in CANON process[J].Biochemical Engineering Journal, 2016,105:306-313.
Kornaros M, Dokianakis S N, Lyberatos G. Partial nitrification/denitrification can be attributed to the slow response of nitrite oxidizing bacteria to periodic anoxic disturbances[J]. Environmental Science & Technology, 2010,44(19):7245-7253.
Ma B, Bao P, Wei Y, et al. Suppressing nitrite-oxidizing bacteria growth to achieve nitrogen removal from domestic wastewater via anammox using intermittent aeration with low dissolved oxygen[J].Scientific Reports, 2015,5:13048.
Qiu S, Wang L F, Chen Z P, et al. An integrated mainstream and sidestream strategy for overcoming nitrite oxidizing bacteria adaptation in a continuous plug-flow nutrient removal process[J].Bioresource Technology, 2021,319:124133.
Bao P, Wang S Y, Ma B, et al. Achieving partial nitrification by inhibiting the activity of Nitrospira-like bacteria under high-DO conditions in an intermittent aeration reactor[J]. Journal of Environmental Sciences, 2017,56:71-78.
Wu J, He C D, van Loosdrecht M C M, et al. Selection of ammonium oxidizing bacteria (AOB) over nitrite oxidizing bacteria (NOB) based on conversion rates[J]. Chemical Engineering Journal, 2016,304:953-961.
Gilbert E M, Agrawal S, Karst S M, et al. Low temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater[J]. Environmental Science & Technology,2014,48(15):8784-8792.