Effect of co-metabolism by polycyclic aromatic hydrocarbon on the microbial degradation of benzo[a]anthracene and its mechanism
ZHU Qing-he1,2, ZENG Jun1, WU Yu-cheng1, YANG-Jie2, LIN Xian-gui1
1. Key Laboratory of Soil Environmental and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. State Environmental Protection Engineering Center for Urban Soil Contamination Control and Remediation, Shanghai Academy of Environmental Sciences, Shanghai 200233, China
Abstract：In this study, the effect of three co-metabolic substrates (phenanthrene, anthracene, pyrene) on environmental distribution of benzo[a]anthracene (BaA) was studied by using 14C isotope tracer. High-throughput sequencing and co-occurrence network analysis were used to evaluate bacterial community responses. The results demonstrated that all the three co-metabolic substrates promoted degradation of BaA. The mineralization of BaA increased by 2.5% in the anthracene treatment compared to the control. In the treatments of phenanthrene and pyrene, the bound residue of 14C was 4% higher than in the control, amounting to 31% of the total addition. The topological characteristics of bacterial co-occurrence network changed with the supplementation of co-metabolic substrates. Anthracene significantly raised the proportion of positive links, which indicated mutualistic interactions. In contrast, the competition between bacterial taxa was strengthened in the treatments of phenanthrene and pyrene, as evidenced by increased negative links after incubation. It was likely that anthracene and BaA degraded through common metabolic pathways, which caused mutualistic interactions and higher mineralization rate of BaA in the soil microcosms. Overall, co-metabolism could be an important factor influencing the fate of BaA in soil and changing the interaction among bacterial species.
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ZHU Qing-he, ZENG Jun, WU Yu-cheng, YANG-Jie, LIN Xian-gui. Effect of co-metabolism by polycyclic aromatic hydrocarbon on the microbial degradation of benzo[a]anthracene and its mechanism. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 808-814.
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