Abstract：To clarify the potential mechanism by which PM2.5 exposure causes liver fibrosis and the key components leading to this adverse effect, 10-month-old C57BL/6 female mice were exposed to PM2.5 from different cities of China (Taiyuan, Beijing, Hangzhou and Guangzhou) for 4weeks. And tissue section staining, qRT-PCR and Western-blot technique were used to evaluate the features of liver fibrosis in mice. Pearson correlation coefficient was used to analyse the correlation between the expression level of liver fibrosis-related genes in mice and the typical chemical composition of PM2.5 from different cities. The results showed that a significant decrease in the organ coefficient of liver was found in Taiyuan and Hangzhou PM2.5-exposed group. Taiyuan PM2.5 exposure significantly increased the area of collagen deposition and the relative expression level of fibrosis related genes (Col1a1, Col3a1, TGF-β and MMP2) in the mice liver, and only PM2.5 from Taiyuan induced the significant increase of Col1a1 protein in mice liver, while PM2.5 from other cities except Taiyuan exposure did not cause any of the above changes in fibrosis related factors. In addition, the results of correlation analysis showed that the metal components such as Cr, Mn, Mo, Cs, Pb, Bi, U and Fe were positively correlated with the mRNA expression of Col1a1 and Col3a1. Except for NA, AC and BaP, the other 15PAHs were significantly correlated with Col1a1 mRNA expression, and the sum of 18EPA PAHs was significantly correlated with Col3a1. The above results indicated that PM2.5 exposure induced hepatic fibrosis in mice, especially PM2.5 from Taiyuan. TGF-β and related signal molecules in its pathway has beenshownto play a vital role in mediating liver fibrosis induced by PM2.5. Cr, Mn, Mo, Cs, Pb, Bi, U, Fe and PAHs of PM2.5 were probably critical components to induce liver fibrosis.
闫志鹏, 李舒月, 邢颀颂, 南楠, 秦国华, 桑楠. 不同城市PM2.5致小鼠肝脏纤维化的效应差异研究[J]. 中国环境科学, 2022, 42(6): 2878-2885.
YAN Zhi-peng, LI Shu-yue, XING Qi-song, NAN Nan, QIN Guo-hua, SANG Nan. Differential Effects of PM2.5from different cities in the induction of hepaticfibrosis in mice. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2878-2885.
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