Start - up of granular - flocculate sludge CANON process based on side - flow FNA treatment
REN Zhi-qiang1, LI Dong1, WANG Wen-qiang1, WANG Tian-shuo1, ZHANG Jie1,2
1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; 2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract：In order to evaluate the feasibility of the free nitrite (FNA) lateral flow treatment of flocculent sludge by initializing the autotrophic nitrogen removal (CANON) processing, this study was conducted to investigate effects of the FNA treatment on ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) activities and the operation efficiency of hydraulically screened flocculent sludge treatment in the granular-flocculation sludge SBR reactor. The results show that after 0.6mg/L FNA treatment of R1 for 30days, the removal rate of NH4+-N returned to the level before the treatment and the short-range nitrification was stable. The average total effluent nitrogen of the system was 13.84mg/L; and the △NO3--N/△NH4+-N ratio was close to the theoretical one of the CANON reaction equation of 0.11, indicating successful start of the CANON process. However, R2, treated with 0mg/LFNA failed to start due to the massive proliferation of NOB. The batch test results confirm that after the treatment of 0.6mg/L FNA, the NOB activity was only 16.39% of the control group (FNA=0mg/L), and the recovery of NOB activity was not found in subsequent operation thus NOB was effectively inhibited. Additionally, the activity of AOB was affected because the removal rate of NH4+-N in the reactor was only 69.69% of that of before treatment and the activity of AOB recovered by only 68.06% after 6h.
任志强, 李冬, 王文强, 王天朔, 张杰. 基于侧流FNA处理的颗粒-絮体污泥CANON启动[J]. 中国环境科学, 2022, 42(9): 4129-4136.
REN Zhi-qiang, LI Dong, WANG Wen-qiang, WANG Tian-shuo, ZHANG Jie. Start - up of granular - flocculate sludge CANON process based on side - flow FNA treatment. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4129-4136.
Isaac O, Andriy M, Elzbieta P. Pre-concentration of ammonium to enhance treatment of wastewater using the partial nitritation/anammox process [J]. Environmental Technology, 2015,36(9-12):1256-1264.
[2]
Ma B, Wang S, Cao S, et al. Biological nitrogen removal from sewage via anammox: Recent advances [J]. Bioresource Technology, 2016, 200(1):981-990.
[3]
Pérez J, Lotti T, Kleerebezem R, et al. Outcompeting nitrite-oxidizing bacteria in single-stage nitrogen removal in sewage treatment plants: A model-based study [J]. Water Research, 2014,66(12):208-218.
[4]
魏 琛,罗固源.FA和pH值对低C/N污水生物亚硝化的影响 [J]. 重庆大学学报(自然科学版), 2006,29(3):124-127. Wei C, Luo G Y. Effects of free ammonium and pH on partial nitrification process [J]. Journal of Chongqing University (Natural Science Edition), 2006,29(3):124-127.
[5]
Yang Q, Peng Y, Liu X, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities [J]. Environmental Science & Technology, 2007,41(23):8159-8164.
[6]
Guo J, Peng Y, Wang S, et al. Long-term effect of dissolved oxygen on partial nitrification performance and microbial community structure [J]. Bioresource Technology, 2009,100(11):2796-2802.
[7]
于德爽,殷金兰,王晓霞,等.控制DO及FA条件下短程硝化过程系统稳定性研究 [J]. 环境工程学报, 2011,5(12):2677-2680. Yu D S, Yin J L, Wang X X, et al. Study on stability of shortcut nitrification by controlling DO and FA [J]. Chinese Journal of Environmental Engineering, 2011,5(12):2677-2680.
[8]
张 亮,张树军,彭永臻.污水处理中游离氨对硝化作用抑制影响研究 [J]. 哈尔滨工业大学学报, 2012,44(2):75-79. Zhang L, Zhang S J, Peng Y Z, et al. Review of study on the effects of free ammonia inhibitions on nitrifying bacteria in wastewater treatment [J]. Journal of Harbin Institute of Technology, 2012,44(2): 75-79.
[9]
吴莉娜,彭永臻,王淑莹,等.游离氨对城市生活垃圾渗滤液短程硝化的影响 [J]. 环境科学, 2008,29(12):3428-3432. Wu L N, Peng Y Z, Wang S Y, et al. Effect of free ammonia on the short-cut nitrification of the municipal landfill leachate [J]. Environmental Science, 2008,29(12):3428-3432.
[10]
傅金祥,汪 洋,杨 勇.FA与FNA对A/O工艺短程硝化处理垃圾渗滤液的影响 [J]. 工业水处理, 2012,32(5):48-51. Fu J X, Wang Y, Yang Y. Effect of FA and FNA on the short-cut nitrification of landfill leachate in an A/O system [J]. Industrial Water Treatment, 2012,32(5):48-51.
[11]
刘 牡,彭永臻,吴莉娜,等.FA与FNA对两级UASB-A/O处理垃圾渗滤液短程硝化的影响 [J]. 化工学报, 2010,61(1):172-179. Liu M, Peng Y Z, Wu L N, et al. Effect of FA and FNA on short-cut nitrification of landfill leachate in two-stage UASB-A/O system [J]. CIESC Journal, 2010,61(1):172-179.
[12]
韩晓宇,张树军,甘一萍,等.以FA与FNA为控制因子的短程硝化启动与维持 [J]. 环境科学, 2009,30(3):809-814. Han X Y, Zhang S J, Gan Y P, et al. Start up and maintain of nitritation by the inhibition of FA and FNA [J]. Environmental Science, 2009,30(3):809-814.
[13]
Wang Q, Ye L, Jiang G, et al. Free Nitrous Acid (FNA)-based pretreatment enhances methane production from waste activated sludge [J]. Environmental Science & Technology, 2013,47(20):11897- 11904.
[14]
Zhou Y, Oehmen A, Lim M, et al. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants [J]. Water Research, 2011,45(15):4672-4682.
[15]
Nan X, Ma B, Qian W, et al. Achieving nitritation by treating sludge with free nitrous acid: The effect of starvation [J]. Bioresource Technology, 2019,271(1):159-165.
[16]
委 燕,王淑莹,马 斌,等.缺氧FNA对氨氧化菌和亚硝酸盐氧化菌的选择性抑菌效应 [J]. 化工学报, 2014,65(10):4145-4149. Wei Y, Wang S Y, Ma B, et al. Selective inhibition effect of free nitrous acid on ammonium oxidizing bacteria and nitrite oxidizing bacteria under anoxic condition [J]. CIESC Journal, 2014,65(10): 4145-4149.
[17]
Jiang C, Xu S, Wang R, et al. Achieving efficient nitrogen removal from real sewage via nitrite pathway in a continuous nitrogen removal process by combining free nitrous acid sludge treatment and DO control [J]. Water Research, 2019,161(11):590-600.
[18]
Miao J, Yin Q, Hori T, et al. Nitrifiers activity and community characteristics under stress conditions in partial nitrification systems treating ammoniumrich wastewater [J]. Journal of Environmental Sciences, 2018,73(11):1-8.
[19]
Wang Q, Ye L, Jiang G, et al. Side-stream sludge treatment using free nitrous acid selectively eliminates nitrite oxidizing bacteria and achieves the nitrite pathway [J]. Water Research, 2014,55(5):245-255.
[20]
Zhang L, Liu M, Zhang S, et al. Integrated fixed-biofilm activated sludge reactor as a powerful tool to enrich anammox biofilm and granular sludge [J]. Chemosphere, 2015,140(12):114-118.
[21]
Stedmon C A, Bro R. Characterizing dissolved organic matter fluorescence with parallel factor analysis: a tutorial [J]. Limnology and Oceanography: Methods, 2008,6(11):572-579.
[22]
郑照明,李 军,侯爱月,等.城市生活污水SNAD生物膜脱氮特性 [J]. 中国环境科学, 2017,37(4):1322-1330. Zheng Z M, Li J, Hou A Y, et al. Nitrogen removal performance of SNAD biofilm cultured by domestic wastewater. [J]. China Environmental Science, 2017,37(4):1322-1330.
[23]
Ma B, Yang L, Wang Q, et al. Inactivation and adaptation of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria when exposed to free nitrous acid [J]. Bioresource Technology, 2017,245 (12):1266-1270.
[24]
杨明明, 刘子涵,周 杨,等.厌氧氨氧化颗粒污泥EPS及其对污泥表面特性的影响 [J]. 环境科学, 2019,40(5):2341-2348. Yang M M, Liu Z H, Zhou Y, et al. Extracellular Polymeric Substances of ANAMMOX Granular Sludge and Its Effects on Sludge Surface Characteristics [J]. Environmental Science, 2019,40(5):2341- 2348.
[25]
李 冬,田海成,梁瑜海,等.水质条件对厌氧氨氧化颗粒污泥EPS含量的影响 [J]. 哈尔滨工业大学学报, 2017,49(2):6-12. Li D, Tian H C, Liang Y H, et al. Effect of water quality of influent on the content of extracellular polymeric substances in anammox granule sludge [J]. Journal of Harbin Institute of Technology, 2017,49(2): 6-12.
[26]
Puñal A, Brauchi S, Reyes J G, et al. Dynamics of extracellular polymeric substances in UASB and EGSB reactors treating medium and low concentrated wastewaters [J]. Water Science and Technology, 2003,48(6):41-49.
[27]
李 冬,曹美忠,郭跃洲,等.不同磷浓度下生物除磷颗粒系统的COD需求 [J]. 环境科学, 2018,39(7):3247-3253. Li D, Cao M Z, Guo Y Z, et al. COD Requirement for Biological Phosphorus Removal Granule System Under Different Phosphorus Concentrations [J]. Environmental Science, 2018,39(7):3247-3253
[28]
曹秀芹,赵自玲.胞外聚合物(EPS)构成的影响因素分析 [J]. 环境科学与技术, 2010,33(12):420-424. Cao X Q, Zhao Z L. Analysis of influencing factor on the component of extracellular polymeric substance (EPS) [J]. Environmental Science & Technology, 2010,33(12):420-424.
[29]
Houghton J I, Stephenson T. Effect of influent organic content on digested sludge extracellular polymer content and dewaterability [J]. Water Research, 2002,36(14):3620-3628.
[30]
张家惠,巩有奎.FNA旁侧污泥预处理SBR-PN过程启动及N2O释放特性 [J]. 环境工程, 2021,39(6):72-78. Zhang J H, Gong Y K. Start-up of SBR-PN process and N2O emission in side pretreatment of wasted sludge by free nitrous acid [J]. Environmental Engineering, 2021,39(6):72-78.
[31]
Nielsen P H, Jahn A, Palmgren R. Conceptual model for production and composition of exopolymers in biofilms [J]. Water Science and Technology, 1997,36(1):11-19.
[32]
Song Y X, Liao Q, Yu C, et al. Physicochemical and microbial properties of settled and floating anammox granules in upflow reactor [J]. Biochemical Engineering Journal, 2017,123(7):75-85.
[33]
Wang W Q, Li D, Li S, et al. Characteristics and mechanism of hollow anammox granular sludge with different settling properties [J]. Journal of Environmental Chemical Engineering, 2022,10(2):107230.
[34]
Wang Z, Zhang S, Zhang L, et al. Restoration of real sewage partial nitritation-anammox process from nitrate accumulation using free nitrous acid treatment [J]. Bioresource Technology, 2018,251(3):341- 349.
[35]
张 昭,李 冬,仲 航,等.常温下低氨氮污水的短程硝化启动研究 [J]. 中国给水排水, 2012,28(9):35-39. Zhang Z, Li D, Zhong H, et al. Start-up experiment of shortcut nitrification for low ammonia nitrogen wastewater at room temperature [J]. China Water&Wastewater, 2012,28(9):35-39.
