环境因子对红胞藻JZB-2降解海水中对二甲苯的影响

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摘要前期研究分离得到一种隐藻门微藻—红胞藻(Rhodomonas sp. JZB-2),能够快速生物降解海水中的PX.为了判断该微藻用于PX污染海域生物修复所需的适宜条件,依次研究4种环境因子(海水pH值、温度、盐度和光强)对该微藻生长及其降解PX的影响.结果表明:各因子均对红胞藻JZB-2生长及其降解PX产生较大影响.pH 7.0~8.5时微藻生长最快,但是最有利于PX降解的pH值为 7.0.微藻生长适宜盐度为14~35,而PX降解速率最大值出现在盐度35时.温度20~30℃时最有利于微藻生长,而PX降解的最适温度为30℃.光强的影响比较特别,在光照条件下,微藻在200~400 μmol/(m²·s)时生长较快,但是PX降解速率在800 μmol/(m²·s)时最大;黑暗条件下微藻无法生长却仍能快速降解PX,这有利于泄漏事故海域生物修复在阴雨天气和夜间正常进行.研究结果将为红胞藻JZB-2在污染海域的合理应用提供依据.

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	姚宇
	李皓
	孟范平

关键词 ：环境因子, 微藻, 海水, 对二甲苯(PX), 生物降解

Abstract：In previous study, Rhodomonas sp. JZB-2, a Cryptophyta microalga, was isolated and tested to be capable of rapid biodegradation of PX in seawater. In order to optimize the suitable conditions for this alga used for the bioremediation of PX in contaminated sea areas, the effects of four environmental factors (seawater pH, temperature, salinity and light intensity) on the microalgal growth and the efficiency of PX degradation were studied. The results showed that each factor significantly affected the microalgal growth and PX degradation. The microalga grew fastest at pH between 7.0 and 8.5, but the highest degradation ratio occurred at pH 7.0. This microalga could growth well at salinity ranging from 14 to 35, and the maximum value of PX degradation rate appeared at the salinity of 35. A range of temperature from 20 to 30℃ was suitable for the microalgal growth, and the degradation ratio of PX peaked at 30℃. A special effect tendency was found for the light intensity. This microalga grew fast under the light intensity of 200~400μmol/(m²·s), however, the maximum degradation rate of PX occurred at 800 μmol/(m²·s). Interestingly, the microalga could not grow under dark condition but still degraded PX rapidly, which was beneficial to its usage in the spill accident sea area on rainy days or at night. This study provided a basis for the rational usage of JZB-2 to degrade PX in the contaminated sea areas.

Key words： environmental factors microalgae seawater para-xylene (PX) biodegradation

收稿日期: 2022-06-15

PACS:

X55

基金资助:国家自然科学基金资助项目(42077335)

通讯作者: 孟范平,教授,mengfanping@ouc.edu.cn E-mail: mengfanping@ouc.edu.cn

作者简介: 姚宇(1998-),女,山东菏泽人,中国海洋大学硕士研究生,主要研究方向为海洋生态修复.

引用本文:

姚宇, 李皓, 孟范平. 环境因子对红胞藻JZB-2降解海水中对二甲苯的影响[J]. 中国环境科学, 2023, 43(1): 181-189. YAO Yu, LI Hao, MENG Fan-ping. Effects of environmental factors on the degradation of paraxylene in seawater by Rhodomonas sp. JZB-2. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(1): 181-189.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I1/181

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