温度对好氧颗粒污泥系统污泥膨胀的影响

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摘要为探究温度对好氧颗粒污泥系统污泥膨胀的影响以及微生物群落结构特征,采用序批式反应器,进水为人工合成废水,利用Illumina测序技术分析10℃、18℃和25℃3种温度下好氧颗粒污泥系统微生物群落结构及细菌的动态变化.结果表明,18℃条件下好氧颗粒污泥沉降性能良好SVI值维持在40mL/g左右.而10和25℃条件下好氧颗粒污泥均发生膨胀现象,SVI值分别为194.67和100mL/g.通过qPCR分析可知,10℃条件下,特定的丝状菌里Sphaerotilus natans丰度值最高,该菌大量繁殖导致SVI值极高.Illumina测序结果表明,温度导致好氧颗粒污泥系统的优势菌属具有显著差异性,18℃条件下,好氧颗粒污泥优势菌属为Tsukamurella和unclassified_f_Comamonadaceae,系统具有良好的污染物去除能力;10℃条件下,其优势菌属为Sphaerotilus,该菌属不能利用复杂的有机物;25℃条件下,优势菌属为Tsukamurella和unclassified_f_Microbacteriacea.从微观角度分析好氧颗粒污泥系统脱氮性能,利用FAPROTAX数据库预测不同温度下细菌的代谢功能差异性,在18℃条件下nitrate reduction和nitrate respiration型氮循环功能菌相对丰度分别为7.81%和7.78%,均高于其它2个好氧颗粒污泥系统,这与18℃下好氧颗粒污泥系统有较好的氮去除效果相符合.

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	欧家丽
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关键词 ：好氧颗粒污泥, 温度, 丝状菌, 微生物群落结构, 细菌代谢功能

Abstract：To explore the effects of temperature on sludge bulking and the characteristics of microbial community structure in aerobic granular sludge system, three sequencing batch reactors were used with artificial wastewater as the influent. Illumina sequencing technology was used to analyze the microbial community structure and dynamic changes of bacteria in aerobic granular sludge system at 10℃,18℃ and 25℃,respectively.The results showed that the particle sludge settling performance of the 18℃ was good, and the SVI maintained at about 40mL/g. But under the conditions of 10℃ and 25℃, aerobic granular sludge were bulked, of which the SVI were 194.67mL/g and 100mL/g, respectively. According to the qPCR analysis, at 10℃, the abundance value of Sphaerotilus Natans was the highest among the specific filamentous bacteria, it mass propagation of this bacterium would leaded to extremely high SVI. Illumina sequencing results showed that temperature caused the significant differences of dominant species among these three systems. The predominant strains at 18℃ were Tsukamurella and unclassified_f_Comamonadaceae, this system had a good pollutant removal ability. And at 10℃, the predominant species was Sphaerotilus, which was unable to utilize complex organic matter. As for the system at 25℃, the dominant bacteria were Tsukamurella and unclassified_f_Microbacteriacea. To analyze the nitrogen removal performance of aerobic granular sludge system from the micro point of view, the FAPROTAX database was used to predict the differences in bacterial metabolic functions at different temperatures. At 18℃, the relative abundance of nitrate reduction and nitrate respiration bacteria, which were kinds of nitrogen cycling functional bacteria, were 7.81% and 7.78%, higher than those in other systems. This results matched the better nitrogen removal effect at the same temperatre.

Key words： aerobic granular sludge temperature filamentous bacteria microbial community structure bacterial metabolic function

收稿日期: 2022-08-30

PACS:

X703

基金资助:国家自然科学基金资助项目(52270017)

通讯作者: 高春娣,教授,gaochundi@bjut.edu.cn E-mail: gaochundi@bjut.edu.cn

作者简介: 欧家丽(1996-),女,广西梧州人,北京工业大学硕士研究生,主要研究方向为丝状菌污泥膨胀与控制.发表论文2篇.oujiali2020@163.com.

引用本文:

欧家丽, 高春娣, 韩颖璐, 杨箫阳, 程丽阳, 彭永臻. 温度对好氧颗粒污泥系统污泥膨胀的影响[J]. 中国环境科学, 2023, 43(4): 1716-1723. OU Jia-li, GAO Chun-di, HAN Ying-lu, YANG Xiao-yang, CHENG Li-yang, PENG Yong-zhen. Effect of temperature on sludge bulking in aerobic granular sludge system. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1716-1723.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I4/1716

[1]	陈颖,陈垚,李聪,等.好氧颗粒污泥结构特点及稳定性研究进展[J]. 工业水处理, 2021,41(10):28-35. Chen Y, Chen Z, Li C, et al. Research progress on structural characteristics and stability of aerobic granular sludge[J]. Industrial Water Treatment, 2021,41(10):28-35.
[2]	Zhang Q G, Hu J J, Lee D J. Aerobic granular processes:Current research trends[J]. Bioresource Technology, 2016,210:74-80.
[3]	Zou J, Tao Y, Li J, et al. Cultivating aerobic granular sludge in a developed continuous-flow reactor with two-zone sedimentation tank treating real and low-strength wastewater[J]. Bioresource Technology, 2018,247:776-783.
[4]	Bengtsson S, De Blois M, Wilen B M, et al. A comparison of aerobic granular sludge with conventional and compact biological treatment technologies[J]. Environmental Technology, 2019,40(21):2769-2778.
[5]	Sarma S J, Tay J H, Chu A. Finding knowledge Gaps Aerobic Granulation Technology[J]. Trends in Biotechnology, 2017,35(1):66-78.
[6]	Moura L L, Duarte K L S, Santlago E P, et al. Strategies to re-establish stable granulation after filamentous outgrowth:Insights from lab-scale experiments[J]. Process Safety and Environmental Protection, 2018, 117:606-615.
[7]	Sarma S J, Tay J H. Aerobic granulation for future wastewater treatment technology:challenges ahead[J]. Environmental Science-Water Research & Technology, 2018,4(1):9-15.
[8]	Franca R D G, Pinheiro H M, Vanloosdrecht M C M, et al. Stability of aerobic granules during long-term bioreactor operation[J]. Biotechnology Advances, 2018,36(1):228-246.
[9]	Munoz-Palazon B, Rodriguez-Sanchez A, Hurtado-Martinez M, et al. Performance and microbial community structure of aerobic granular bioreactors at different operational temperature[J]. Journal of Water Process Engineering, 2020,33:101110.
[10]	Ab Halim M H, Nor Anuar A, Abdul Jamal N S, et al. Influence of high temperature on the performance of aerobic granular sludge in biological treatment of wastewater[J]. Journal of Environmental Management, 2016,184(Pt 2):271-280.
[11]	梁东博,卞伟,王文啸,等.低温条件下好氧颗粒污泥培养及其脱氮性能研究[J]. 中国环境科学, 2019,39(2):634-640. Liang D B, Bian W, Wang W X, et al. Aerobic granular sludge formation and nutrients removal characterics under low temperature[J]. China Environmental Science, 2019,39(2):634-640.
[12]	尚越飞,王申,宗倪,等.污水生物处理工艺低温下微生物种群结构[J]. 环境科学, 2020,41(10):4636-4643. Shang Y F, Wang S, Zong N, et al. Microbial population structure of wastewater biological treatment process at low temperature[J]. Environmental Science, 2020,41(10):4636-4643.
[13]	Fan N, Wang R, Qi R, et al. Control strategy for filamentous sludge bulking:Bench-scale test and full-scale application[J]. Chemosphere, 2018,210:709-716.
[14]	Eikelboom D H. Process control of activated sludge plants by microscopic investigation[M]. London, UK:IWA Publishing, 2000:127-143.
[15]	Grau P, Da-Rin B P. Management of toxicity effects in a large wastewater treatment plant[J]. Water Science and Technology, 1997, 36(2/3):1-8.
[16]	Nancharaiah Y V, Reddy G K K. Aerobic granular sludge technology:Mechanisms of granulation and biotechnological applications[J]. Bioresource Technology, 2018,247:1128-1143.
[17]	Figueroa M, Val Del Rio A, Campos J L, et al. Filamentous bacteria existence in aerobic granular reactors[J]. Bioprocess and Biosystems Engineering, 2015,38(5):841-851.
[18]	国家环境保护总局.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002:210-276. State Environmental Protection Administration. Analytical methods for water and wastewater monitoring[M]. (Fourth Edition). BeiJing:China Environmental Science Press, 2002:210-276.
[19]	张著,高大文,袁向娟,等.营养物质缺乏引起的好氧颗粒污泥膨胀及其恢复[J]. 环境科学, 2012,33(9):3197-3201. Zhang Z, Gao D W, Yuan X J, et al. Bulking and recovery of aerobic granular sludge caused by nutrient deficiency[J]. Environmental Science, 2012,33(9):3197-3201.
[20]	高春娣,韩颖璐,程丽阳,等.温度冲击引起的丝状菌污泥膨胀菌群特征[J]. 中国环境科学, 2022,42(6):2680-2689. Gao C D, Han Y L, Cheng L Y, et al. Microbial community characteristics of filamentous sludge bulking at temperature shocking environment[J]. China Environmental Science, 2022,42(6):2680-2689.
[21]	Kent T R, Bott C B, Wang Z W. State of the art of aerobic granulation in continuous flow bioreactors[J]. Biotechnology Advances, 2018, 36(4):1139-1166.
[22]	Gao D, Liu L, Liang H, et al. Aerobic granular sludge:characterization, mechanism of granulation and application to wastewater treatment[J]. Critical Reviews in Biotechnology, 2011,31(2):137-152.
[23]	Van Dijk E J H, Pronk M, Van Loosdrecht M C M. Controlling effluent suspended solids in the aerobic granular sludge process[J]. Water Research, 2018,147:50-59.
[24]	Sharaf A, Guo B, Liu Y. Impact of the filamentous fungi overgrowth on the aerobic granular sludge process[J]. Bioresource Technology Reports, 2019,7:100272.
[25]	王杰,彭永臻,杨雄,等.温度对活性污泥沉降性能与微生物种群结构的影响[J]. 中国环境科学, 2016,36(1):109-116. Wang J, Peng Y Z, Yang X, et al. Effect of temperature on activated sludge settleability and microbial community structure[J]. China Environmental Science, 2016,36(1):109-116.
[26]	高春娣,张娜,韩徽,等.低温下丝状菌膨胀污泥的微生物多样性[J]. 环境科学, 2020,41(7):3373-3383. Gao C D, Zhang N, Han H, et al. Microbial diversity of filamentous bulking sludge at low temperature[J]. Environmental Science, 2020, 41(7):3373-3383.
[27]	Su J F, Zhang Y M, Liang D H, et al. Performance and microbial community of an immobilized biofilm reactor (IBR) for Mn(II)-based autotrophic and mixotrophic denitrification[J]. Bioresource Technology, 2019,286:121407.
[28]	He Q, Zhang J, Gao S, et al. A comprehensive comparison between non-bulking and bulking aerobic granular sludge in microbial communities[J]. Bioresour Technol, 2019,294:122151.
[29]	Wang P, Yu Z, Qi R, et al. Detailed comparison of bacterial communities during seasonal sludge bulking in a municipal wastewater treatment plant[J]. Water Research, 2016,105:157-166.
[30]	Ou D, Li W, Li H, et al. Enhancement of the removal and settling performance for aerobic granular sludge under hypersaline stress[J]. Chemosphere, 2018,212:400-407.
[31]	Tang X, Guo Y, Jiang B, et al. Metagenomic approaches to understanding bacterial communication during the anammox reactor start-up[J]. Water Research, 2018,136:95-103.
[32]	Ye L, Shao M-F, Zhang T, et al. Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing[J]. Water Research, 2011,45(15):4390-4398.
[33]	Xu J, He J, Wang M, et al. Cultivation and stable operation of aerobic granular sludge at low emperature by sieving out the batt-like sludge[J]. Chemosphere, 2018,211:1219-1227.
[34]	Gonzalez-Martinez A, Munoz-Palazon B, Maza-Marquez P, et al. Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature[J]. Bioresource Technology, 2018,256:22-29.
[35]	Mcilroy S J, Starnawska A, Starnawski P, et al. Identification of active denitrifiers in full-scale nutrient removal wastewater treatment systems[J]. Environmental Microbiology, 2016,18(1):50-64.
[36]	Sun F, Sun B, Hu J, et al. Organics and nitrogen removal from textile auxiliaries wastewater with A(2)O-MBR in a pilot-scale[J]. Journal of Hazardous Materials, 2015,286:416-424.
[37]	Bassin J P, Kleerebezem R, Dezotti M, et al. Simultaneous nitrogen and phosphate removal in aerobic granular sludge reactors operated at different temperatures[J]. Water Research, 2012,46(12):3805-3816.
[38]	Yuan S, Gao M, Zhu F, et al. Disintegration of aerobic granules during prolonged operation[J]. Environmental Science-Water Research & Technology, 2017,3(4):757-766.
[39]	Aqeel H, Basuvaraj M, Hall M, et al. Microbial dynamics and properties of aerobic granules developed in a laboratory-scale sequencing batch reactor with an intermediate filamentous bulking stage[J]. Applied Microbiology and Biotechnology, 2016,100(1):447-460.
[40]	Jun L, Jun L, Kai X, et al. Role of adding dried sludge micropowder in aerobic granular sludge reactor with extended filamentous bacteria[J]. Bioresource Technology Reports, 2019,5:51-58.
[41]	Zhang X, Zheng S, Xiao X, et al. Simultaneous nitrification/denitrification and stable sludge/water separation achieved in a conventional activated sludge process with severe filamentous bulking[J]. Bioresource Technology, 2017,226:267-271.