硝化微颗粒污泥的快速培养及其对硫化物的响应特性

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摘要在连续流气提内循环反应器中接种城市污水厂脱水污泥,考察硝化颗粒污泥快速培养及其对硫化物的响应特性.结果表明,在高接种污泥浓度(19.3g/L)条件下,通过逐步缩短HRT(7h降至2h)和提升进水氨氮负荷(0.5kg/(m³·d)升至3.1kg/(m³·d)),30d内基本实现污泥颗粒化,SVI₃=SVI₃₀为(16.8±0.3)mL/g,颗粒污泥平均粒径266μm;高浓度硝化颗粒污泥[(11.9±0.2)g/L]保证了系统的高负荷运行(氨氮去除负荷(2.4±0.3)kg/(m³·d)).硫化物对颗粒污泥中氨氧化细菌(AOB)和亚硝酸盐氧化细菌(NOB)均有明显抑制,但AOB活性恢复明显快于NOB,硝化微颗粒污泥实现亚硝化功能,当硫化物浓度升至10mg/L时,亚硝化率达80%左右;然而,进一步提高硫化物浓度至20mg/L,亚硝化率无显著提升;第82d时的批次实验表明,AOB活性随初始硫化物浓度的升高而降低,NOB活性却呈上升趋势,推测与颗粒污泥中AOB(外层)和NOB(内层)的分层分布密切相关.此外,硫化物对小粒径(<0.3mm)硝化颗粒污泥的影响更为显著.

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	王彩霞
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	刘文如

关键词 ：硝化微颗粒污泥, 硫化物, 连续流, 脱氮性能

Abstract：The dewatered sludge of municipal wastewater treatment plant was inoculated in a continuous flow gas lift internal circulation reactor, and the rapid culture of nitrified granular sludge and its response to sulfides were investigated. The results showed that under the condition of high inoculation sludge concentration (19.3g/L), sludge granulation was basically achieved within 30 days by gradually shortening HRT (7h to 2h) and increasing influent ammonia nitrogen load (0.5kg/(m³·d) to 3.1kg/(m³·d)). The SVI₃=SVI₃₀ was (16.8±0.3)mL/g, and the average particle size of granular sludge was 266 μm. The high concentration nitrification granular sludge[(11.9±0.2)g/L] ensured the high load operation of the system (ammonia nitrogen removal load (2.4±0.3)kg/(m³·d)). Sulfides in the influent significantly inhibited ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in granular sludge, but the recovery of AOB activity was faster than that of NOB, so nitrifying micro-granular sludge achieved the function of nitrification. When the sulfide concentration increased to 10mg/L, the nitrification rate reached about 80%. However, when the sulfide concentration was further increased to 20mg/L, the nitrification rate did not increase significantly. The batch experiments on the 82nd day showed that AOB activity decreased with the increase of initial sulfide concentration, while NOB activity increased, which was closely related to the stratified distribution of AOB (outer layer) and NOB (inner layer) in granular sludge. In addition, the effect of sulfides on nitrifying granular sludge with small particle size (<0.3mm) was more significant.

Key words： nitrifying microgranular sludge sulfide continuous flow nitrogen removal performance

收稿日期: 2022-09-15

PACS:

X703

基金资助:国家自然科学基金项目(51808367);城市生活污水资源化利用技术国家地方联合工程实验室开放课题项目(2018KF05)

通讯作者: 刘文如,副教授,liuwenru1987@126.com E-mail: liuwenru1987@126.com

作者简介: 王彩霞(1995-),女,甘肃临夏人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文5篇.2161550346@qq.com.

引用本文:

王彩霞, 陈洁, 王倩, 沈耀良, 刘文如. 硝化微颗粒污泥的快速培养及其对硫化物的响应特性[J]. 中国环境科学, 2023, 43(5): 2277-2285. WANG Cai-xia, CHEN Jie, WANG Qian, SHEN Yao-liang, LIU Wen-ru. Rapid cultivation of nitrifying microgranular sludge and its response to sulfides. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(5): 2277-2285.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I5/2277

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