Performance of iron scrap to alleviate the inhibition of sulfides on denitrification by anaerobic ammoxidation
CHEN Jie1, WANG Cai-xia1, WANG Qian1, SHEN Yao-liang1,2,3, LIU Wen-ru1,2,3
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215000, China; 2. National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215000, China; 3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215000, China
Abstract：The long-term inhibition of sulfide on anaerobic ammonia oxidation (Anammox) system and the effect of adding scrap iron on the system performance were studied in an up-flow anaerobic sludge bed reactor (UASB). Results showed that low concentration (<10mg/L) of sulfide had no significant effect on the system performance. When its concentration increased to 20, 30 and 40mg/L, the nitrogen removal rate (NRR) of the reactor decreased by 13%, 42% and 51%, respectively. The high concentration (40mg/L) of sulfide reduced the specific anaerobic ammonia oxidation activity (SAA) by 63%. Anammox biomass had adaptability to the sulfide inhibition. Moreover, the long-term operation with sulfides caused the deterioration of particle structure, sludge floating, and caking. The addition of iron scrap significantly alleviated the inhibition of sulfide, and quickly recovered the sludge settleability and the reactor nitrogen removal efficiency. After 19 days of iron scrap addition, the NRR increased by 20% (up to 4.43kg/(m3·d)) compared with the sulfur-free stage, and the SAA reduced only by 16.8%. The microbial community analysis showed that the increased sulfide concentrations caused the decrease of the relative abundance of Candidatus Kuenenia from 18.81% to 7.31%. The relative abundance of Candidatus Kuenenia at the bottom layer of the reactor was 15 times higher than that at the top layer after adaptation. At the same time, the denitrifying bacteria (Arenimonas and Thiobacillus) were enriched. After adding scrap iron, the abundance of Ca. Kuenenia decreased slightly, but the relative abundance of Ca. Kuenenia at the top layer was higher than that at the bottom layer. The relative abundance of Thiobacillus in the system increased by 3.36 times, and the relative abundance of denitrifying bacteria in the bottom layer also increased. This study provides a new idea for promoting the application of Anammox process in sulfide containing wastewater.
陈洁, 王彩霞, 王倩, 沈耀良, 刘文如. 废铁屑缓解硫化物抑制厌氧氨氧化脱氮性能的研究[J]. 中国环境科学, 2023, 43(4): 1636-1645.
CHEN Jie, WANG Cai-xia, WANG Qian, SHEN Yao-liang, LIU Wen-ru. Performance of iron scrap to alleviate the inhibition of sulfides on denitrification by anaerobic ammoxidation. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(4): 1636-1645.
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