Abstract：In this experiment, Sequencing batch reactor (SBR) was used for simultaneous nitrogen and sulfur removal. Chemical control group (sterilization, without sludge) and biological test group (adding sludge) were set up to study the yield and characteristics of elemental sulfur in the process of simultaneous nitrite and sulfide removal. In the chemical control group, removal percentages of nitrite and sulfide could reach 25.07% and 62.26%, respectively, and main products were ammonia nitrogen and thiosulfate, in which there was no elemental sulfur produced; while in the biological test, removal percentage of nitrite and sulfide could reach 100% and 99.94%, respectively, and the main products were elemental sulfur and nitrogen gas in the appropriate concentration range (60~180mg S/L). As influent sulfide concentration was 180mg S/L, the production of elemental sulfur was as high as 79.58%. The produced elemental sulfur was existed in the form of orthorhombic sulfur (S8) with negative surface charge, and the particle size of elemental sulfur had a normal distribution.
孙月, 陈柯序, 王凯权, 楼菊青, 王如意, 李强标, 蔡靖. 亚硝酸盐型同步脱氮除硫工艺单质硫产率及特性[J]. 中国环境科学, 2021, 41(3): 1228-1233.
SUN Yue, CHEN Ke-xu, WANG Kai-quan, LOU Ju-qing, WANG Ru-yi, LI Qiang-biao, CAI Jing. Yield and characteristics of elemental sulfur in the process of simultaneous nitrite and sulfide removal. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(3): 1228-1233.
Chen C, Ren N Q, Wang A J, et al. Simultaneous biological removal of sulfur, nitrogen and carbon using EGSB reactor[J]. Applied Microbiology and Biotechnology, 2008,78(6):1057-1063.
[2]
Chen Z, Zhang S H, Zhong L X. Simultaneous sulfide removal, nitrogen removal and electricity generation in a coupled microbial fuel cell system[J]. Bioresource Technology, 2019,291:121888.
[3]
Yuan Y, Bian A Q, Chen F, et al. Continuous sulfur biotransformation in an anaerobic-anoxic sequential batch reactor involving sulfate reduction and denitrifying sulfide oxidization[J]. Chemosphere, 2019,234:568-578.
[4]
Michal Sposob, Rune Bakke, Carlos Dinamarca. Metabolic divergence in simultaneous biological removal of nitrate and sulfide for elemental sulfur production under temperature stress[J]. Bioresource Technology, 2017,233:209-215.
[5]
Watsuntorn W, Ruangchainikom C, Rene E R, et al. Comparison of sulphide and nitrate removal from synthetic wastewater by pure and mixed cultures of nitrate-reducing, sulphide-oxidizing bacteria[J]. Bioresource Technology, 2019,272:40-47.
[6]
黄勇,袁怡.高氨氮、硫酸盐废水的生物脱氮除硫研究新进展[J]. 中国给水排水, 2010,26(10):5-8. Huang Y, Yuan Y. Biological nitrogen and sulfur removal from wastewater with high-concentration ammonia nitrogen and sulphate[J]. China Water & Wastewater, 2010,26(10):5-8.
[7]
Wang J Q, Hua M L, Li Y F, et al. Achieving high nitrogen removal efficiency by optimizing nitrite-dependent anaerobic methane oxidation process with growth factors[J]. Water Research, 2019, 161:35-42.
[8]
Cai J, Zheng P, Mahmood Q. Influence of various nitrogenous electron acceptors on the anaerobic sulfide oxidation[J]. Bioresource Technology, 2010,101(9):2931-2937.
[9]
王威,毕志浩,张若晨,等.自养反硝化脱氮耦合沼气同步脱硫效能研究[J]. 环境科学学报, 2019,39(10):3291-3301,3565. Wang W, Bi Z H, Zhang R C, et al. The study on efficiency of autotrophic denitrification coupling biogas desulfurization simultaneously[J]. Acta Scientiae Circumstantiae, 2019,39(10):3291-3301,3565.
[10]
李军,张文文,王立军,等.硫自养反硝化同步脱氮除硫启动试验[J]. 沈阳建筑大学学报(自然科学版), 2010,26(1):162-165. Li J, Zhang W W, Wang L J, et al. Study on the start-up of sulfur autotrphic denitrification bioreactor for the simultaneous removal of nitrogen and sulfur. Journal of Shenyang Jianzhu University(Natural Science), 2010,26(1):162-165.
[11]
蔡靖,郑平,胡宝兰,等.硫氮比对厌氧生物同步脱氮除硫工艺性能的影响[J]. 环境科学学报, 2008,28(8):1506-1514. Cai J, Zheng P, Hu B L, et al. The effect of sulfide to nitrate ratio on the performance of a simultaneous anaerobic sulfide and nitrate removal process[J]. Acta Scientiae Circustantiae, 2008,28(8):1506-1514.
[12]
Mahmood Q, Zheng P, Cai J, et al. Anoxic sulfide bio-oxidation using nitrite as electron acceptor[J]. Journal of Hazardous Materials, 2007, 147(1):249-256.
[13]
蔡靖,郑平.亚硝酸盐型同步厌氧生物脱氮除硫工艺的运行性能[J]. 生物工程学报, 2009,23(11):1684-1689. Cai J, Zheng P. Simultaneous removal of sulfide and nitrite by anaerobic bioprocess[J]. Chinese Journal of Biotechnology, 2009, 23(11):1684-1689.
[14]
Gilles P, Sébastien M, Nicolas B. Nitrate and nitrite reduction of a sulphide-rich environment[J]. Journal of Chemical Technology & Biotechnology, 1998,72(3):213-220.
[15]
Li W J, Zhang M, Kang D. Mechanisms of sulfur selection and sulfur secretion in a biological sulfide removal (BISURE) system[J]. Environment International, 2020,137:105549.
[16]
国家环境保护总局.《水和废水监测分析方法》(第四版)[M]. 北京:中国环境科学出版社, 2002. State Environmental Protection Administration. "Water and Wastewater Monitoring and Analysis Methods"[M]. 4th Ed. Beijing:China Environmental Science Press, 2002.
[17]
熊建华.紫外分光光度法测定溶液中硫代硫酸钠含量[J]. 医药导报, 2004,11:866. Xiong J H. Determination of sodium thiosulfate content in solution by ultraviolet spectrophotometry[J]. Herald of Medicine, 2004,11:866
[18]
李军幸,张克强,季民,等.分光光度法测定微生物处理含硫化物废水中的单质硫含量[J]. 农业环境科学学报, 2006,(1):261-265. Li J X, Zhang K Q, Ji M, et al. Spectrophotometric Quantification of Sulfur in Sulfide-containing Wastewater Treated with Microbial[J]. Journal of Agro-Environment Science, 2006,(1):261-265.
[19]
云玉攀,杨鑫,李子富,等.催化还原法深度处理污水中硝态氮的研究[J]. 中国环境科学, 2015,35(11):3310-3316. Yun Y P, Yang X, Li Z F, et al. Study on advanced treatment for removing nitrate in wastewater through chemical catalytic reduction[J]. China Environmental Science, 2015,35(11):3310-3316.
[20]
蔡靖,郑平,胡宝兰,等. pH和碱度对同步厌氧生物脱氮除硫工艺性能的影响[J]. 化工学报, 2008,5:1264-1270. Cai J, Zheng P, Hu B L, et al. Influence of pH and alkalinity on process performance of simultaneous anaerobic sulfide and nitrate removal[J]. CIESC Journal, 2008,5:1264-1270.
[21]
霍小爱,袁林江,罗大成,等.亚硝酸盐对乳酸发酵缺氧-好氧SBR脱氮系统除磷的影响[J]. 环境科学学报, 2019,39(12):3966-3972. Huo X A, Yuan L J, Luo D C, et al. The effect of nitrite on the phosphorus removal in anoxic-aerobic SBR system with lactic acid fermentation[J]. Acta Scientiae Circumstantiae, 2019,39(12):3966-3972.
[22]
Burgin A J, Stephen K, Hamilton. et al. Have we overemphasized the role of denitrification in aquatic ecosystems? A review of nitrate removal pathways[J]. Frontiers in Ecology and the Environment, 2007,5(2):89-96.
[23]
远野,王爱杰,马素丽,等.反硝化脱硫工艺中生物硫分布特征及分离方法[J]. 哈尔滨工业大学学报, 2014,46(8):34-39. Yuan Y, Wang A J, Ma S L, et al. Distribution characteristics and separation of biological sulfur in denitrifying sulfide removal process[J]. Journal of Harbin Institute of Technology, 2014,46(8):34-39.
[24]
Cai J, Zheng P, Mahmood Q, et al. Elemental sulfur recovery of biological sulfide removal process from wastewater:A review[J]. Critical Reviews in Environmental Science and Technology, 2017, 47(21):2079-2099.
[25]
Janssen A J, De Keizer A, Lettinga G. Colloidal properties of a microbiologically produced sulphur suspension in comparison to a LaMer sulphur sol[J]. Colloids and Surfaces B Biointerfaces, 1994, 3(1/2):111-117.
[26]
Janssen A, Keizer A D, Aelst A V, et al. Surface characteristics and aggregation of microbiologically produced sulphur particles in relation to the process conditions[J]. Colloids and Surfaces B Biointerfaces, 1996,6(2):115-129.
[27]
陈帆.高流速下反硝化脱硫工艺负荷优化及单质硫回收研究[D]. 哈尔滨:哈尔滨工业大学, 2016. Chen F. Research on load optimization and elemental sulfur recovery at high up-flow velocity for denitrifying sulfide removal[D]. Harbin:Harbin Institute of Technology, 2016.
[28]
张克强,黄文星,季民,等.含硫化物废水生物处理过程中单质硫的形成特性[J]. 农业环境科学学报, 2006,2:522-526. Zhang K Q, Huang W X, Ji M, et al. Characteristics of microbiologically sulfur producing during biological treatment for wastewater containing sulfide[J]. Journal of Agro-Environment Science, 2006,2:522-526.
[29]
Moiler R H, Jacobs C. Buparvaquone mucoadhesive nanosuspension:preparation, optimization and long-term stability[J]. International Journal of Pharmaceutics, 2002,237(1/2):151-161.
