Abstract:The growth, surface morphology, photosynthetic pigment content, chlorophyll fluorescence parameters, antioxidant system and cell cycle of Phaeodactylum tricornutum in different concentrations of EE2 (0.375, 0.75, 1.5, 3, 6mg/L) were determined. The results show that EE2 could inhibit the growth of Phaeodactylum tricornutum at the 96h-EC50 level of 5.32mg/L, suggesting that EE2 had high toxicity to Phaeodactylum tricornutum. Low concentration of EE2≤0.75mg/L promoted the photosystem II (PSII) activity of Phaeodactylum tricornutum; however, its surface morphology was damaged and its photosynthetic pigment content and maximal photochemical efficiency (Fv/Fm) were significantly reduced under EE2 concentration of 6mg/L. Meanwhile, the light limitation slope (α), the maximum electron transfer efficiency (rETRmax), and semi-saturated light intensity (Ik) also decreased. These results indicate that high concentration of EE2 inhibited the photosystem II (PSII) activity of Phaeodactylum tricornutum. In addition, the activities of both the superoxide dismutase (SOD) and catalase (CAT) and the malondialdehyde (MDA) content significantly increased under the high concentration of EE2, and the cell cycle was arrested in the DNA synthesis phase (S) and division phase (G2/M), demonstrating that a stress response was resulted from the antioxidant system of Phaeodactylum tricornutum.
Cabana H, Jones J P, Agathos S N. Elimination of endocrine disrupting chemicals using White Rot Fungi and their lignin modifying enzymes:A review[J]. Engineering in Life Sciences, 2007,7(5):429-456.
[2]
Bradley P M, Barber L B, Chapelle F H, et al. Biodegradation of 17β-Estradiol, Estrone and Testosterone in stream sediments[J]. Environmental Science & Technology, 2009,43(6):1902-1910.
[3]
Chang H, Wan Y, Hu J Y. Determination and source apportionment of five classes of steroid hormones in Urban Rivers[J]. Environmental Science & Technology, 2009,43(20):7691-7698.
[4]
Xu W H, Yan W, Huang W X, et al. Endocrine-disrupting chemicals in the Pearl River Delta and coastal environment:sources, transfer, and implications[J]. Environmental Geochemistry and Health, 2014, 36(6):1095-1104.
[5]
Casey F X M, Hakk H, Simůnek J, et al. Fate and transport of testosterone in agricultural soils[J]. Environmental Science & Technology, 2004,38(3):790-798.
[6]
Liu Y H, Zhang S H, Ji G X, et al. Occurrence, distribution and risk assessment of suspected endocrine-disrupting chemicals in surface water and suspended particulate matter of Yangtze River (Nanjing section)[J]. Ecotoxicology and Environmental Safety, 2017,135:90-97.
[7]
Yin G G, Kookana R S, Ru Y J. Occurrence and fate of hormone steroids in the environment[J]. Environment International, 2002, 28(6):545-551.
[8]
Fan D L, Yin W, Gu W, et al. Occurrence, spatial distribution and risk assessment of high concern endocrine-disrupting chemicals in Jiangsu Province, China[J]. Chemosphere, 2021,285:131396-131396.
[9]
Gorga M, Insa S, Petrovic M, et al. Occurrence and spatial distribution of EDCs and related compounds in waters and sediments of Iberian rivers[J]. Science of the Total Environment, 2015,503-504:69-86.
[10]
Thrupp T J, Runnalls T J, Scholze M, et al. The consequences of exposure to mixtures of chemicals:Something from 'nothing' and 'a lot from a little' when fish are exposed to steroid hormones[J]. Science of the Total Environment, 2018,619-620:1482-1492.
[11]
Almeida N, Silva M G, Soares A, et al. Concentrations levels and effects of 17alpha-Ethinylestradiol in freshwater and marine waters and bivalves:A review[J]. Environmental Research, 2020,185:109316.
[12]
Aris A Z, Shamsuddin A S, Praveena S M. Occurrence of 17α-ethynylestradiol (EE2) in the environment and effect on exposed biota:A review[J]. Environment International, 2014,69:104-119.
[13]
Nasuhoglu D, Berk D, Yargeau V. Photocatalytic removal of 17α-ethinylestradiol (EE2) and levonorgestrel (LNG) from contraceptive pill manufacturing plant wastewater under UVC radiation[J]. Chemical Engineering Journal, 2012,185-186:52-60.
[14]
Czarny K, Szczukocki D, Krawczyk B, et al. Toxic effects of single animal hormones and their mixtures on the growth of Chlorella vulgaris and Scenedesmus armatus[J]. Chemosphere, 2019,224:93-102.
[15]
Rodrigues S, Silva A M, Antunes S C. Assessment of 17α-ethinylestradiol effects in Daphnia magna:life-history traits, biochemical and genotoxic parameters[J]. Environmental Science and Pollution Research, 2021,28(18):23160-23173.
[16]
Nash J P, Kime D E, Van der Ven L T, et al. Long-term exposure to environmental concentrations of the pharmaceutical ethynylestradiol causes reproductive failure in fish[J]. Environmental Health Perspect, 2004,112(17):1725-1733.
[17]
Xin X Y, Huang G, Zhang B Y. Review of aquatic toxicity of pharmaceuticals and personal care products to algae[J]. Journal of Hazardous Materials, 2020,410:124619.
[18]
Liu Y, Guan Y T, Gao Q T, et al. Cellular responses, biodegradation and bioaccumulation of endocrine disrupting chemicals in marine diatom Navicula incerta[J]. Chemosphere, 2010,80(5):592-599.
[19]
Chen L Z, Zhou L N, Liu Y D, et al. Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii[J]. Ecotoxicology and Environmental Safety, 2012,84:155-162.
[20]
杨慧丽,段舜山.邻苯二甲酸二丁酯对三角褐指藻的生态毒性效应[J].生态环境学报, 2010,19(9):2155-2159. Yang H L, Duan S S. The ecological toxic effects of dibutyl phthalate on Phaeodactylum tricornutum[J]. Ecology and Environmental Sciences, 2010,19(9):2155-2159.
[21]
Silva C P, Otero M, Esteves V. Processes for the elimination of estrogenic steroid hormones from water:A review[J]. Environmental Pollution, 2012,165:38-58.
[22]
HJ/T 154-2004新化学物质危害评估导则[S]. HJ/T 154-2004 The guidelines for the hazard evaluation of new chemical substances[S].
[23]
Zhong X, Donws C A, Che X K, et al. The toxicological effects of oxybenzone, an active ingredient in suncream personal care products, on prokaryotic alga Arthrospira sp. and eukaryotic alga Chlorella sp[J]. Aquatic Toxicology, 2019,216:105295.
[24]
于延珍,张丽,曹为,等.四溴双酚A (TBBPA)对叉鞭金藻的毒性效应研究[J].海洋科学进展, 2016,34(3):421-429. Yu Y Z, Zhang L, Cao W, et al. Toxic Effects of Tetrabromobisphenol A (TBBPA) on dicrateria inornata[J]. Advances in Marine Science, 2016,34(3):421-429.
[25]
Singh S P, Singh P. Effect of temperature and light on the growth of algae species:A review[J]. Renewable and Sustainable Energy Reviews, 2015,50:431-444.
[26]
Belhaj D, Athmouni K, Frikha D et al. Biochemical and physiological responses of halophilic nanophytoplankton (Dunaliella salina) from exposure to xeno-estrogen 17α-ethinylestradiol[J]. Environmental Science and Pollution Research International, 2017,24(8):7392-7402.
[27]
Pocock T, Falk S. Negative impact on growth and photosynthesis in the green alga Chlamydomonas reinhardtii in the presence of the estrogen 17α-ethynylestradiol[J]. PLoS ONE, 2017,9(10):e109289.
[28]
杨璨,于晓娟,王欣泽,等.双酚A对铜绿微囊藻生长及生理的影响[J].安全与环境工程, 2014,21(5):21-25. Yang C, Yu X J, Wang X Z, et al. Effects of bisphenol A on the growth and physiology microcystis aeruginosa[J]. Safety and Environmental Engineering, 2014,21(5):21-25.
[29]
Genty B, Briantais J, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 1989,990(1):87-92.
[30]
李根,管超,安民,等.壬基酚对三角褐指藻的毒性效应及其机理[J].生态科学, 2013,32(3):298-302. Li G, Guan C, An M, et al. Toxic effect of nonylphenol on Phaeodactylum triconutum and its mechanism[J]. Ecological Science, 2013,32(3):298-302.
[31]
Calabrese E J, Baldwin L A, Holland C D. Hormesis:a highly generalizable and reproducible phenomenon with important implications for risk assessment[J]. Risk Anal, 1999,19(2):261-281.
[32]
李卓娜,孟范平,赵顺顺,等.BDE-47对2种海洋微藻光合特性的影响[J].中国环境科学, 2010,30(2):233-238. Li Z N, Meng F P, Zhao S S, et al. Effects of BDE-47 on the chlorophyll fluorescence parameters of two species of marine microalgae[J]. China Environmental Science, 2010,30(2):233-238.
[33]
管超,孙志伟,安民,等.壬基酚对球形棕囊藻的生态毒性效应[J].生态环境学报, 2011,20(4):640-645. Guan C, Sun Z W, An M, et al. The ecological toxic effects of Nonylphenol on Phaeocystis globosa[J]. Ecology and Environmental Sciences, 2011,20(4):640-645.
[34]
Wang M X, Zhang Y X, Guo P Y. Effect of florfenicol and thiamphenicol exposure on the photosynthesis and antioxidant system of Microcystis flos-aquae[J]. Aquatic Toxicology, 2017,186:67-76.
[35]
曲玉楹,张彩杰,沈秋岑,等.吐纳麝香对东海原甲藻的毒性作用机制[J].中国环境科学, 2022,42(3):1401-1409. Qu Y Y, Zhang C J, Shen Q C, et al. Toxic mechanism of tonalide on Prorocentrum donghaiense[J]. China Environmental Science, 2022, 42(3):1401-1409.
[36]
吴义诚,吴文惠,陈国元,等.壬基酚对小球藻生理及光合特性的影响[J].安全与环境学报, 2020,20(3):1185-1190. Wu Y C, Wu W H, Chen G Y, et al. Effect of nonylphenol on the physiological and photosynthetic characteristics of Chlorella vulgaris[J]. Journal of Safety and Environment, 2020,20(3):1185-1190.
[37]
Wan J J, Guo P Y, Peng X F, et al. Effect of erythromycin exposure on the growth, antioxidant system and photosynthesis of Microcystis flos-aquae[J]. Journal of Hazardous Materials, 2015,283:778-786.
[38]
刘正宇,许钧盛,孙田力,等.四溴联苯醚对米氏凯伦藻生长及光合生理的影响[J].海洋湖沼通报, 2021,43(2):124-130. Liu Z Y, Xv J S, Sun T L, et al. Toxin effects of tetrabromodiphenyl ethers to population growth and photosynthetic physiology of Karenia mikimotoi[J]. Transactions of Oceanology and Limnology, 2021,43(2):124-130.
[39]
Chen Z W, Wang J, Chen H, et al. Enantioselective phytotoxicity of dichlorprop to arabidopsis thaliana:The effect of cytochrome P450enzymes and the role of Fe[J]. Environmental Science & Technology, 2017,51(20):12007-12015.
[40]
Wu L, Qiu Z H, Zhou Y, et al. Physiological effects of the herbicide glyphosate on the cyanobacterium Microcystis aeruginosa[J]. Aquatic Toxicology, 2016,178:72-79.
[41]
Wan J J, Guo P Y, Zhang S X. Response of the cyanobacterium Microcystis flos-aquae to levofloxacin[J]. Environmental Science and Pollution Research International, 2014,21(5):3858-3865.
[42]
Xie X J, Zhou Q X, Lin D S, et al. Toxic effect of tetracycline exposure on growth, antioxidative and genetic indices of wheat (Triticum aestivum L.)[J]. Environmental Science and Pollution Research International, 2011,18(4):566-575.
[43]
Huang B, Tang J, He H, et al. Ecotoxicological effects and removal of 17β-estradiol in chlorella algae[J]. Ecotoxicology and Environmental Safety, 2019,174:377-383.
[44]
Zhang W, Xiong B, Sun W F, et al. Acute and chronic toxic effects of bisphenol A on Chlorella pyrenoidosa and Scenedesmus obliquus[J]. Environmental Toxicology, 2014,29(6):714-722.
[45]
Meng X R, Wang F, Li Y F, et al. Comparing toxicity and biodegradation of racemic glufosinate and L-glufosinate in green algae Scenedesmus obliquus[J]. Science of the Total Environment, 2022,823:153791-153791.
[46]
刘伟杰,吴孝情,鄢佳英,等.壬基酚对羊角月牙藻的毒性效应研究[J].中国环境科学, 2018,38(6):2329-2336. Liu W J, Wu X Q, Yan J Y et al. Toxic effects of nonylphenol on Selenastrum capricornutum[J]. China Environmental Science, 2018,38(6):2329-2336.
[47]
马春艳,郭丽丽,梁前进,等.双酚-A和17β-雌二醇对人乳腺癌细胞生长的影响[J].中国环境科学, 2002,22(5):408-411. Ma C Y, Guo L L, Liang Q J, et al. Influences of bisphenol-A and 17β-estradiol on the growth of human breast cancer cell (MCF-7)[J]. China Environmental Science, 2002,22(5):408-411.
[48]
董婷,嵇源源,王剑文.活性氧参与多壁碳纳米管诱导的RAW264.7细胞毒性[J].生态毒理学报, 2013,8(1):55-60. Dong T, Ji Y Y, Wang J W. Involvement of reactive oxygen species in multi-walled carbon nanotubes (MWCNTs)-induced cytotoxicity to RAW264.7Cells[J]. Asian Journal of Ecotoxicology, 2013,8(1):55-60.
[49]
Wei Y, Zhu N, Lavoie M, et al. Copper toxicity to Phaeodactylum tricornutum:A survey of the sensitivity of various toxicity endpoints at the physiological, biochemical, molecular and structural levels[J]. Biometals:An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine, 2014,27(3):527-537.
[50]
王美仙.淡水微藻对水体颗粒物与抗生素及其复合污染胁迫的响应研究[D].厦门:华侨大学, 2017. Wang M X. Study the response of freshwater microalgae to suspended particles, antibiotics and combined stress[D]. Xiamen:Huaqiao University, 2017.