根系分泌物对生物强化修复PAHs污染土壤的影响

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摘要通过土壤微宇宙实验,探究了生物强化对多环芳烃(PAHs)污染土壤的修复效果及根系分泌物对生物强化修复的影响.结果表明,向土壤中接种PAHs降解菌群(布鲁氏菌(Brucella sp.)、苍白杆菌(Ochrobactrum sp.)、鞘脂单胞菌(Sphingomonas sp.)、克雷伯氏菌(Klebsiella sp.)和不动杆菌(Acinetobacter sp.))21d后, PAHs残留量较对照下降了15.8%;在此基础上添加黑麦草实际或模拟根系分泌物均显著增强了生物强化修复效果, PAHs残留量较对照分别下降了26.3%和24.6%. 3种生物强化措施均改变了细菌群落结构,其中添加根系分泌物后的处理细菌群落结构的改变更大,芳烃降解基因丰度增加得更多,显示根系分泌物通过促进PAHs降解菌的生长影响细菌群落从而提升生物强化修复效果.两类根系分泌物对细菌群落影响的差别未引发其对生物强化促进效果的差异.该研究可为优化有机污染土壤生物强化修复技术提供参考.

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	李瑜婷
	鲍文秀
	张闻
	王加宁
	黄玉杰
	宋繁永

关键词 ：多环芳烃, 土壤修复, 黑麦草, 根系分泌物, 生物强化

Abstract：A soil microcosm experiment was conducted to investigate the effect of bioaugmentation on polycyclic aromatic hydrocarbons (PAHs)-contaminated soil and the influence of root exudates on bioaugmentation. The content of PAHs residue was decreased by 15.8% with PAHs-degrading bacteria (Brucella, Ochrobactrum, Sphingomonas, Klebsiella and Acinetobacter sp.) compared to CK in 21d. The addition of ryegrass or artificial root exudates significantly enhanced the bioaugmentation effect and the contents were decreased by 26.3% and 24.6% compared to CK, respectively. These 3bioaugmentation treatments altered the soil bacterial community structures. Compared with the inoculation of PAHs-degrading bacteria alone, the addition of root exudates changed the community structure more significantly and increased the abundance of aromatic compounds degradation-related genes more markedly, indicating that the root exudates affected the bacterial community by promoting the growth of PAHs-degrading bacteria and thus enhanced the bioremediation effect. The different effects of the two types of root exudates on soil bacterial community did not lead to a difference in their contribution to the promotion for bioaugmentation. This study can provide a reference for optimizing bioaugmentation remediation techniques for organic-contaminated soils.

Key words： Polycyclic aromatic hydrocarbons (PAHs) soil remediation ryegrass root exudates bioaugmentation

收稿日期: 2023-04-25

PACS:

X53

基金资助:国家重点研发计划项目(2019YFC1804103)；山东省自然科学基金资助项目(ZR2022MD116)

通讯作者: 张闻,副研究员,zw-sunshine@163.com E-mail: zw-sunshine@163.com

作者简介: 李瑜婷(1998-),女,山东泰安人,硕士研究生,研究方向为有机污染土壤修复及微塑料的环境效应.发表论文1篇.2233605485@qq.com

引用本文:

李瑜婷, 鲍文秀, 张闻, 王加宁, 黄玉杰, 宋繁永. 根系分泌物对生物强化修复PAHs污染土壤的影响[J]. 中国环境科学, 2023, 43(8): 4183-4193. LI Yu-ting, BAO Wen-xiu, ZHANG Wen, WANG Jia-ning, HUANG Yu-jie, SONG Fan-yong. Effects of root exudates on bioaugmentation of PAHs-contaminated soil. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 4183-4193.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I8/4183

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