土壤中石油烃检测方法比较及降解动力学分析

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摘要通过比较重量法、紫外分光光度法及气相色谱法对石油烃污染土壤原样及石油烃高效降解菌生物修复土壤中石油烃含量进行检测,气相色谱法灵敏度高、准确度高,作为测定污染土壤石油烃含量的方法更为稳定而准确;能定量分析不同碳数石油烃含量,有效地反映不同降解阶段土壤微生物修复过程中石油烃的降解情况.对微生物修复污染土壤时的石油烃降解动力学进行研究,结果表明:污染土壤中石油烃的降解率与时间的变化趋势符合二级动力学模型,动力学方程式的相关系数达到0.942,该菌剂的半衰期为29d,符合实验观察到的降解趋势.

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	梁庆霞
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	霍乾伟
	陈贯虹

关键词 ：石油烃, 检测方法, 生物降解, 降解动力学分析

Abstract：Gravimetry, ultraviolet spectrophotometry and gas chromatography were used to detect and analyze the components of the original soil contaminated by petroleum hydrocarbons and the soil bioremediated by petroleum hydrocarbon degrading bacteria. By comparison, it is found that gas chromatography has high sensitivity and accuracy, and is more stable as a method to determine petroleum hydrocarbon content in contaminated soil. By combining the utilization of each component of petroleum hydrocarbon pollutants by microorganisms, it showed that this method could effectively reflect the microbial decline. The degradation kinetics of petroleum hydrocarbons in the contaminated soil was studied. The results showed that the degradation rate of petroleum hydrocarbons in the contaminated soil was consistent with the second-order kinetic model. The correlation coefficient of the kinetic equation reached about 0.942, and the half-life of the bactericide was 29d, which was consistent with the degradation trend observed in the experiment.

Key words： petroleum hydrocarbons detection methods biodegradation degradation kinetics analysis

收稿日期: 2023-04-25

PACS:

X53

基金资助:国家重点研发计划项目(2019YFC1804100)

通讯作者: 陈贯虹,研究员,cgh@qlu.edu.cn E-mail: cgh@qlu.edu.cn

作者简介: 梁庆霞(1998-),女,山东日照人,硕士,主要从事固体废弃物资源化研究.liangqingxia1998@163.com

引用本文:

梁庆霞, 王加宁, 周方园, 黄玉杰, 霍乾伟, 陈贯虹. 土壤中石油烃检测方法比较及降解动力学分析[J]. 中国环境科学, 2023, 43(8): 4194-4201. LIANG Qing-xia, WANG Jia-ning, ZHOU Fang-yuan, HUANG Yu-jie, HUO Qian-wei, CHEN Guan-hong. Comparison of methods for the detection of petroleum hydrocarbon fractions and analysis of degradation kinetics. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 4194-4201.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I8/4194

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