Effects of InP/ZnS QDs on cartilage development in rare minnow (Gobiocypris rarus) offspring
WU Ying-yi, CHEN Hang, XIE Wei-wei, JIN Li
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, College of Life Sciences, Southwest University, Chongqing 400715, China
Abstract：Quantum Dots (QDs) are a class of nanomaterials. With the wide application of QDs, its toxic affects on organisms are also concerned. In this experiment, InP/ZnS quantum dots were used, and female rare minnows (Gobiocypris rarus) was used as the experimental animal. Three experimental groups (200, 400, 800nmol/L) were designed. Eggs were taken and fertilized at 4 and 7 days of quantum dots exposure. Using embryo fertilization rate, survival rate, body length and full length of larvae as indicators, the larvae were observed by Albion blue dye and Hematoxylin Eosin (HE) staining. The transcript expression levels of bone developmental related genes (bmp2b, sox9, runx2b) were detected to study the effect of quantum dots on the offspring cartilage development of rare minnows. The results showed that the body length and full length of larvae in high concentration group decreased by 6.2% and 5.9%. The PQ-Meckel’s angle increased by 24.8%, mandibular length decreased by 14.6% and 15.2%, and mandibular width decreased by 10.0% and 10.7%. Craniofacial chondrocytes were swelled and reduced in number. It was found that QDs had different affects on the transcript expression levels of related genes at different development time. In conclusion, InP/ZnS quantum dots can affect the skeletal development of offspring of rare minnows.
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