悬移质泥沙-微囊藻毒素复合体对大型溞的毒性效应

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摘要为探究悬移质泥沙(SPM)吸附微囊藻毒素(MCs)后形成的复合体对大型溞生理特性的影响,选择MCs的典型异构体MC-LR,通过吸附动力学实验制备了不同MC-LR吸附量的SPM-MC-LR复合体,研究了复合体对大型溞(Daphnia magna)的急性毒性作用.结果表明,SPM对MC-LR的吸附过程符合拟二级动力学模型和Freundlich等温模型,拟合最大吸附量为1720μg/g,是一种以化学吸附为主导的多分子层吸附且较容易发生.SPM-MC-LR复合体对大型溞的固定率和抗氧化酶活性呈正向剂量和时间依赖,大型溞固定率最高可达93.77%;但当复合体浓度较高时(MC-LR剂量为73.52,94.53μg/g),在大型溞体内引起的氧化应激程度过高会抑制抗氧化酶活性(SOD和CAT活性较24h分别降低了21.44%、26.51%和6.2%、18.27%).相比于游离的MC-LR,SPM的存在可能增强了MC-LR对大型溞的毒性作用.

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关键词 ：悬移质泥沙, 微囊藻毒素, 大型溞, 毒性效应

Abstract：In order to investigate the toxic effects of suspended particulate matter and microcystins complexes on Daphnia magna, the most frequently detected variants of microcystin, MC-LR, was selected and the suspended particulate matter (SPM) and MC-LR complexes were prepared. The acute toxicity of MC-LR adsorbed SPM (SPM-MC-LR) on Daphnia magna was also studied. The results showed that the adsorption process of MC-LR by SPM fitted well with the pseudo-second-order kinetic model and Freundlich isothermal adsorption model, and a maximum adsorption capacity of 1720μg/g was determined. This multimolecular layer adsorption dominated by chemical adsorption was relatively easy to occur. SPM-MC-LR influenced the immobilization rate (the maximum immobilization rate reached 93.77%) and the activity of antioxidant enzymes of Daphnia magna, with positively dose and time dependent manner. However, when the MC-LR content reached 73.52 and 94.53μg/g on the SPM surface, the oxidative stress triggered in the Daphnia magna would inhibit the activities of antioxidant enzymes. As a result, the activities of SOD and CAT after 48h exposure to SPM-MC-LR were decreased by 21.44%, 26.51% and 6.2%, 18.27% in comparison to that after 24h exposure, respectively. Compared with single MC-LR, the presence of SPM may enhance the toxicity of MC-LR toward Daphnia magna.

Key words： suspended particulate matter microcystins Daphnia magna toxic effect

收稿日期: 2023-07-28

PACS:

X524

基金资助:国家自然科学基金资助项目(51609024,41877472, U20A20326)

作者简介: 冯琴霜(1999-),女,湖北武汉人,重庆大学环境与生态学院硕士研究生,主要从事污染物环境效应相关研究.qsfeng0805@163.com.

引用本文:

冯琴霜, 黄维, 何强, 李宏. 悬移质泥沙-微囊藻毒素复合体对大型溞的毒性效应[J]. 中国环境科学, 2024, 44(3): 1687-1696. FENG Qin-shuang, HUANG Wei, HE Qiang, LI Hong. Toxic effects of suspended particulate matter and microcystins complexes on Daphnia magna. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1687-1696.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I3/1687

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