1. Key Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes/Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; 2. Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China
Abstract：To assess the toxic impacts of bisphenol AF (BPAF) on the growth and development of marine medaka (Oryzias melastigma) larvae and whether these effects could recover when environmental restored, the larvae were exposed to BPAF at the concentrations of 7.96 and 82.91μg/L for 7 days, followed by a 7-day recovery in BPAF-free seawater. The survival, growth, heart rate, and swimming behavior, as well as transcription levels of genes related to nervous, cardiovascular, and antioxidant system, were examined. The results showed that exposure to 82.91μg/L BPAF significantly reduced the survival rate; exposure to both of 7.96 and 82.91μg/L BPAF significant inhibited the growth, swimming behavior, and heart rate. After a 7-day recovery, the growth of the larvae recstored, but swimming behavior and heart rate remained suppressed. The results of gene expression analysis showed that, exposure to 7.96μg/L of BPAF significantly downregulated the expression of the neurological gene ache (30%), cardiovascular gene tbx2b (47%), and antioxidant system gene cat (56%), with the inhibitory effects on achee and cat dissipating following recovery; exposure to 82.91μg/L of BPAF significantly reduced the expression of neurological genes syn2a (80%), shha (58%), xdh (59%), gap43 (47%), and elav13 (54%), as well as cardiovascular genes atp2a1 (44%), crhr1 (84%), tbx2b (60%), arnt2 (32%), and tbx6 (68%); following a 7 day recovery, elav13 normalized while gap43 was upregulated, and antioxidant genes cat, sod, cox, and gpx were decreased by 61%, 71%, 51%, and 53% relative to controls. This study provides important data for the assessment of marine ecological risks of BPAF and the establishment of water quality standards.
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