双酚S和双酚F诱导斑马鱼的神经发育毒性及联合作用分析

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摘要本研究选择亚致死剂量下等效应的BPS和BPF,从斑马鱼的发育表型、运动行为、血管发育和神经毒性等4个方面比较神经发育毒性的差异和联合毒性研究.结果表明,虽然LC5的BPS和BPF均会诱导斑马鱼表观畸形,如抑制了斑马鱼游泳囊、卵黄囊等发育;BPS和BPF的暴露会影响斑马鱼的早期运动行为,这与心血管系统损伤以及神经中枢系统损伤有着潜在的关联.BPS和BPF造成了斑马鱼心血管发育的异常,尾静脉(CV)宽度缩短;DA、AChE以及NO神经递质的异常传递导致了斑马鱼神经元发育受损,这对侧线神经系统的功能产生了直接的影响,进而导致斑马鱼早期运动行为的受损. BPF暴露更容易诱导斑马鱼的组织、器官毒性,而BPS直接毒性影响要小于BPF,更多的通过分子层次表现出来,这表明两者作用模式具有一定程度的差异性.此外,结合浓度加和模型的联合作用分析来分析20个检测指标,BPS和BPF产生加和作用有16个,达到了80%,因此混合物产生的毒性效应应当引起重视.研究结果为BPS、BPF及其混合物暴露后的健康风险评估与风险预警给予相关的参考依据.

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	杨潇
	包志伟
	韩晓雯
	苏心聪
	孟令浩
	代卓雅
	王泽君
	王慧利

关键词 ：双酚F, 双酚S, 神经毒性, 联合暴露, 浓度加和模型

Abstract：In this research, BPS and BPF with sublethal dose were selected to compare the difference and combined toxicity of neurodevelopmental toxicity in zebrafish from four aspects: developmental phenotype, motor behavior, vascular development and neurotoxicity. The results showed that both BPS and BPF of LC5 induced apparent deformities in zebrafish, such as inhibiting the development of swimming sac and yolk sac. Exposure to BPS and BPF can affect the early motor behavior of zebrafish, which is potentially associated with damage to the cardiovascular system and nervous central system. BPS and BPF caused abnormal cardiovascular development and shortened caudal vein (CV) width in zebrafish. The abnormal transmission of DA, AChE and NO neurotransmitters leads to the impaired development of zebrafish neurons, which has a direct impact on the function of the lateral nervous system, and then leads to the impaired early motor behavior of zebrafish. BPS and BPF show different toxicity differences in specific indicators. BPF exposure is more likely to induce tissue and organ toxicity in zebrafish, while the direct toxicity of BPS is less than that of BPF, which is more manifested at the molecular level, indicating that there is a certain degree of difference in the mode of action of the two. In addition, combined with the analysis of the combined action of concentration addition model, the addition action between BPS and BPF is mainly. The results of this study provide a reference for health risk assessment and risk warning after exposure to BPS, BPF and their mixtures.

Key words： bisphenol F bisphenol S neurotoxicity combined exposure concentration addition model

收稿日期: 2024-07-17

PACS:

X503

基金资助:国家自然科学基金资助项目(22306141,32371705)

通讯作者: 王泽君,讲师,wangzj@usts.edu.cn E-mail: wangzj@usts.edu.cn

作者简介: 杨潇(1997-),男,浙江宁波人,苏州科技大学环境科学与工程学院硕士研究生.主要从事风险评价与生态安全方面的研究.1172418986@qq.com.

引用本文:

杨潇, 包志伟, 韩晓雯, 苏心聪, 孟令浩, 代卓雅, 王泽君, 王慧利. 双酚S和双酚F诱导斑马鱼的神经发育毒性及联合作用分析[J]. 中国环境科学, 2025, 45(2): 1088-1098. YANG Xiao, BAO Zhi-wei, HAN Xiao-wen, SU Xin-cong, MENG Ling-hao, DAI Zhuo-ya, WANG Ze-jun, WANG Hui-li. Neurodevelopmental toxicity and combined effects of bisphenol S and bisphenol F induced zebrafish. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(2): 1088-1098.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I2/1088

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