Abstract：Combining the principle of laser-induced fluorescence and optical fiber biosensing, a portable smartphone powered fluorescence biosensor was constructed for rapid and sensitive on-site detection of antibiotics in waterby integrating an all fiber optical system, a miniaturized flow sampling system, a highly sensitive micro-photoelectric detection system, and a smartphone based APP software. Taking Norfloxacin (NOR), a kind of the quinolone antibiotic, for example, the detection limit of the proposed biosensor was 0.35μg/L and the linear detection range was 1.6~20.6μg/L under optimized conditions. The established NOR detection method was used for the NOR recovery detection in various spiked water samples. The recovery rate of NOR was 85%~120% and a detection period was less than 15min, indicating that this biosensor could be used for rapid and sensitive on-site detection of NOR in actual samples. Combining emerging technologies, such as the Internet of Things, the detection results can be directly uploaded to the environmental monitoring and supervision center according to the set procedures, providing important technology support to achieve early warning and emergency monitoring of water environmental safety.
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