Isolation and identification of epiphytic bacteria from Vallisneria natans and screening of functional strains
LIANG Shu-ya1,2, CUI Chen-chen1, HU Si-wen1,2, ZENG Jin2, DING Cheng-cheng3, CUI Yi-bin3, ZHAO Da-yong1
1. The National Key Laboratory of Water Disaster Prevention, Hohai University, Nanjing 210098, China; 2. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; 3. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
Abstract：In order to explore the functional characteristics of epiphytic bacteria in submerged macrophytes, Vallisneria natans was selected to domesticate the bacterial community in indoor simulated wastewater, screening the rhizosphere and phyllosphere bacteria isolated from V. natans, combined with the 16S rRNA gene sequence analysis and identification results, 172 strains were isolated, purified and preserved (distributed in 5 phyla, 15 order and 42 genera), including 107 rhizosphere strains and 65 phyllosphere strains. 82 representative strains (distributed in 5 phyla, 30 genera and 47 species) were selected for nitrogen removal, phosphorus solubilization, indoleacetic acid production and organic matter removal function validation experiments. Finally, 10 high-efficiency ammonia nitrogen removal strains, 9 high-efficiency nitrate nitrogen removal strains, 12 strains with high-efficiency inorganic phosphorus-solubilizing ability, 11 strains with high-efficiency organic phosphorus-solubilizing ability, 11 strains of high IAA production and 10 high-efficiency organic matter removal strains were screened. The diversity of cultivable microorganisms in the rhizosphere and phyllosphere of V. natans is relatively abundant, the high-efficiency functional strains screened in this study have enriched our cognizance of the resources of epiphytic microorganisms in V. natans, laying the foundation for the development and application of submerged macrophytes synthetic microbial communities.
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