砷和全氟辛酸对赤子爱胜蚓的联合毒性效应

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摘要将赤子爱胜蚓(Eisenia fetida)暴露于亚致死剂量的砷酸盐(As(V))与全氟辛酸(PFOA)的单一及复合污染土壤中28d,分析2种毒物的生物积累和蚯蚓体内多种生物标志物的响应特征,以评估As(V)和PFOA的联合毒性效应.生物富集分析结果表明,土壤中As(V)和PFOA的共存可以增加砷的生物积累量,同时减少PFOA的生物积累.随着暴露水平的增加,大多数生物标志物都表现出显著变化,表明蚯蚓生长受到抑制,并产生了氧化损伤.采用综合生物标志物响应指数(IBR)来整合多种生物标志物响应,发现在As(V)与PFOA的单一及复合污染组中均存在显著的剂量-效应关系.之后,结合IBR和两种复合毒性指数,包括效应加和指数(EAI)和浓度加和指数(CAI),以评估As(V)与PFOA之间的毒性相互作用.结果表明,As(V)和PFOA的联合毒性效应取决于As(V)的暴露浓度,As(V)浓度较高时,复合污染在整个效应水平范围内表现为协同作用,而As(V)浓度较低时的复合毒性则随着效应水平的增加表现出从拮抗作用到协同作用的变化特征.As(V)是对蚯蚓生物标志物造成毒性影响的主要污染物,并影响了As(V)与PFOA的联合毒性效应.本文结果为As(V)和PFOA联合毒性的风险评估提供了有价值的信息.

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	牛晓宇
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关键词 ：蚯蚓, 全氟辛酸, 砷, 生物累积, 综合生物标志物响应指数(IBR), 联合毒性

Abstract：Earthworms (Eisenia fetida) were exposed to sub-lethal doses of arsenate (As(V)), perfluorooctanoic acid (PFOA), and their mixture for 28 days to analyze the bioaccumulation of two toxins and to characterize the response of multiple biomarkers, thereby assessing the combined toxic effects of As(V) and PFOA. The results of bioaccumulation analyses showed that the coexistence of As(V) and PFOA in soil increased arsenic bioaccumulation while decreasing PFOA's bioaccumulation. As exposure levels increased, the majority of the biomarkers demonstrated significant alterations, indicating inhibition of earthworm growth and oxidative damage. The Integrated Biomarker Response Index (IBR) was utilized to summarize multiple biomarker responses, revealing significant dose-effect relationships in both the single and combined contamination groups of As(V) and PFOA. Subsequently, IBR was combined with two mixture toxicity indices, the Effect Addition Index (EAI) and the Concentration Addition Index (CAI), to assess the toxic interaction between As(V) and PFOA. The results indicated that the joint toxicity of As(V) and PFOA was dependent on the concentration of As(V). At higher concentrations of As(V), a synergistic interaction was observed across all effect levels. Conversely, at lower concentrations of As(V), the interaction shifted from antagonism to synergism as the exposure level increased. As(V) emerged as the primary toxicant, significantly impacting earthworm biomarkers and influencing the overall toxicity of the As(V)/PFOA mixture. These findings provide valuable insights for the risk assessment of the combined toxicity of As(V) and PFOA.

Key words： Eisenia fetida PFOA arsenic bioaccumulation integrated biomarker response (IBR) joint toxicity

收稿日期: 2024-02-02

PACS:

X503.2

基金资助:山东省自然科学基金资助项目(ZR2023MD011);国家自然科学基金资助项目(41907351);山东省高等学校青创团队计划资助项目(2022KJ317)

通讯作者: 王志峰,副教授,wangzhifeng18@sdjzu.edu.cn E-mail: wangzhifeng18@sdjzu.edu.cn

作者简介: 牛晓宇(2001-),女,山东枣庄人,山东建筑大学硕士研究生,主要从事土壤环境毒理学研究.nxy1003@126.com.

引用本文:

牛晓宇, 薛维纳, 李帅, 杨丽丽, 王志峰. 砷和全氟辛酸对赤子爱胜蚓的联合毒性效应[J]. 中国环境科学, 2024, 44(10): 5855-5865. NIU Xiao-yu, XUE Wei-na, LI Shuai, YANG Li-li, WANG Zhi-feng. The combined toxic effects of arsenic and perfluorooctanoic acid on the earthworm Eisenia fetida. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(10): 5855-5865.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I10/5855

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