Effects of GAOs abundance to the systemmatic phosphorus enrichment performance and the metabolic characteristics in phosphorus-accumulating biofilm
BI Zhen1, QIAN Meng-meng1, YUAN Yi-na1, WANG Xue-ling1, SONG Ge1, HUANG Yong1,2
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; 2. National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China
Abstract：In a sequencing batch reactor with alternating anaerobic/aerobic operation, activated sludge from an oxidation ditch was used as the inoculated sludge for culturing poly-phosphate biofilm enrichment. The potential functions of GAOs in the biofilm phosphorus enrichment system were investigated by examining the effects of the changes in the abundance of glycogen-accumulating organisms (GAOs) in the biofilm on the phosphorus enrichment performance and the metabolic characteristics of the microbial community. The results showed that GAOs became the dominant organisms in the enrichment culture of phosphorus-accumulating biofilm, but they did not adversely affect the phosphorus removal and enrichment of the biofilm system. Due to the significant increase of PHA metabolic activity and poly-P metabolic activity in individual cells of phosphorus-accumulating organisms (PAOs), the biofilm community as a whole was dominated by the phosphorus accumulating metabolism (PAM). GAOs as a dominant bacterial genus in the system, might obtain the reducing power (NADH) required for synthesizing PHA through EMP metabolism, which could provide sufficient energy reserve for the absorption of phosphorus by the PAOs in aerobic conditions, and thus stimulate the phosphorus removal and enrichment effect in the biofilm system. The inorganic phosphorus transport system (pst) and poly-P synthesizing genes (ppk) were up-regulated, so that the biofilm system showed good phosphorus removal and enrichment ability, and the GAOs (Candidatus Competibacter), as a potential denitrifying bacterium, could synchronize with the aerobic denitrification in the biofilm phosphorus-enrichment system.
毕贞, 钱萌萌, 袁艺娜, 王雪玲, 宋歌, 黄勇. GAOs丰度变化对聚磷生物膜系统的影响——磷富集性能及代谢特征为例[J]. 中国环境科学, 2025, 45(3): 1601-1611.
BI Zhen, QIAN Meng-meng, YUAN Yi-na, WANG Xue-ling, SONG Ge, HUANG Yong. Effects of GAOs abundance to the systemmatic phosphorus enrichment performance and the metabolic characteristics in phosphorus-accumulating biofilm. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1601-1611.
[1] Desmidt, Evelyn, Zhang Y, et al. Global phosphorus scarcity and full-scale P-recovery techniques:a review[J]. Critical Reviews in Environmental Science and Technology, 2015,45.4:336-384. [2] Bi Z, Liu Y, Rutoh W C, et al. Influence of operation sequences on phosphorus recovery by polyphosphate-accumulating organisms biofilm:Performance, kinetics and metabolic response[J]. Journal of Water Process Engineering, 2024,61:105356. [3] 毕贞,张胜,付豪,等.氨氮浓度对生物膜磷富集效果的影响[J].中国环境科学, 2023,43(7):3447-3453. Bi Z, Zhang S, Fu H, et al. Effect of ammonia nitrogen concentration on the phosphorus enrichment effect of biofilm[J]. China Environmental Science, 2023,43(7):3447-3453. [4] Coats ER, Deyo B, Brower N, et al. Effects of anaerobic HRT and VFA loading on the kinetics and stoichiometry of enhanced biological phosphorus removal[J]. Water Environment Research, 2021,93.9:1608-1618. [5] Izadi P, Andalib M. Anaerobic zone functionality, design and configurations for a sustainable EBPR process:A critical review[J]. Science of the Total Environment, 2023,870:162018. [6] Izadi P, Izadi P, Eldyasti A. Understanding microbial shift of enhanced biological phosphorus removal process (EBPR) under different dissolved oxygen (DO) concentrations and hydraulic retention time (HRTs)[J]. Biochemical Engineering Journal, 2021,166:107833. [7] Izadi P, Izadi P, Eldyasti A. A review of biochemical diversity and metabolic modeling of EBPR process under specific environmental conditions and carbon source availability[J]. Journal of Environmental Management, 2021,288:112362. [8] Wang L. The metabolism of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) system under the tropical climate. 2021[J]. [9] 武静.聚磷生物膜富集培养及吸-释磷性能强化[D].苏州:苏州科技大学, 2022. Wu J. Enhancement of phosphorus-absorbing and releasing properties of polyphosphate biofilm[D]. Suzhou:Suzhou University of Science and Technology, 2022. [10] Santos J M, Martins A, Barreto S, et al. Long-term simulation of a full-scale EBPR plant with a novel metabolic-ASM model and its use as a diagnostic tool[J]. Water Research, 2020,187:116398. [11] Wang Z, Li W, Li H, et al. Phylogenomics of Rhodocyclales and its distribution in wastewater treatment systems[J]. Scientific reports, 2020,10.1:3883. [12] Mardanov A V, Gruzdev EV, Smolyakov DD, et al. Genomic and metabolic insights into two novel Thiothrix species from enhanced biological phosphorus removal systems[J]. Microorganisms, 2020,8.12:2030. [13] Kondrotaite Z, Valk LC, Petriglieri F, et al. Diversity and ecophysiology of the genus OLB8 and other abundant uncultured Saprospiraceae genera in global wastewater treatment systems[J]. Frontiers in Microbiology, 2022,13:917553. [14] 张小玲,张萌,陈紫薇,等.内碳源短程反硝化启动及EPD-ANAMMOX耦合工艺性能[J].中国环境科学, 2022,42(2):601-611. Zhang X, Zhang M, Chen Z, et al. Initiation of short-range denitrification with internal carbon source and performance of coupled EPD-ANAMMOX process[J]. China Environmental Science, 2022, 42(2):601-611. [15] 李璐,邵鸿渝,黄继会,等.聚磷菌发生代谢迁移的环境因子及机理分析[J].中国环境科学, 2024,44(5):2642-2651. Li L, Shao H, Huang J, et al. Analysis of the environmental factors and mechanism of metabolic transport of phosphorus-colonizing bacteria[J]. China Environmental Science, 2024,44(5):2642-2651. [16] Bi Z, Wu J, Huang Y, et al. Influence of dissolved oxygen on phosphorus removal by polyphosphate-accumulating organisms biofilm:Performance and metabolic response[J]. Biochemical Engineering Journal, 2023,199:109048. [17] Nittami T, Mukai M, Uematsu K, et al. Effects of different carbon sources on enhanced biological phosphorus removal and "Candidatus Accumulibacter" community composition under continuous aerobic condition[J]. Applied microbiology and biotechnology, 2017,101:8607-8619. [18] Welles L, Abbas B, Sorokin DY, et al. Metabolic response of "Candidatus Accumulibacter Phosphatis" clade II C to changes in influent P/C ratio[J]. Frontiers in microbiology, 2017,7:2121. [19] Chen Y, Li L, Zhang Y, et al. Phosphorus absorption and release in biofilm sequencing batch reactor:The combined action of cells and extracellular polymeric substances and the characteristics of polymer metabolism[J]. Journal of Water Process Engineering, 2022,49:102979. [20] Zhang H, Bi Z, Pan Y, et al. Enhanced phosphorus storage in suspended biofilm by increasing dissolved oxygen[J]. Science of the Total Environment, 2020,722:137876. [21] 毕贞,付豪,王雪玲,等.复杂碳源对生物膜系统磷富集及微生物群落的影响[J].中国环境科学, 2024,44(11):6096-6104. Bi Z, Fu H, Wang X, et al. Effects of complex carbon sources on phosphorus enrichment and microbial communities in biofilm systems[J]. China Environmental Science, 2024,44(11):6096-6104. [22] 李璐,张玥,邵鸿渝,等.侧流与主流磷回收工艺对比及调控因子分析[J].中国环境科学, 2024,44(1):103-113. Li L, Zhang Y, Shao H, et al. Comparison of sidestream and mainstream phosphorus recovery processes and analysis of regulatory factors[J]. China Environmental Science, 2024,44(1):103-113. [23] Zhang X, Zhao B, An Q, et al. The influence of different nitrate concentrations on aerobic sludge granulation and the role of extracellular polymeric substances[J]. Journal of Environmental Management, 2023,348:119226. [24] 李冬,解一博,高飞雁,等.调控内生正磷酸盐强化好氧颗粒污泥脱氮除磷[J].中国环境科学, 2023,43(10):5139-5147. Li D, Xie Y, Gao F, et al. Regulation of endogenous orthophosphate to enhance nitrogen and phosphorus removal by aerobic granular sludge[J]. China Environmental Science, 2023,43(10):5139-5147. [25] 王晓霞,王淑莹,赵骥,等.SPNED-PR系统内PAOs-GAOs的竞争关系及其氮磷去除特性[J].中国环境科学, 2018,38(2):551-559. Wang X, Wang S, Zhao Z, et al. Competitive relationship between PAOs-GAOs and their nitrogen and phosphorus removal characteristics within SPNED-PR system[J]. China Environmental Science, 2018,38(2):551-559. [26] Apha. Standard methods for the examination of water and wastewater, 21st ed[R]. American Public Health Association, 2012, Washington, DC. [27] Oehmen A, Yuan Z, Blackall L L, et al. Comparison of acetate and propionate uptake by polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Biotechnology and bioengineering, 2005,91.2:162-168. [28] Yang W, Shan J, Pan Y, et al. A new strategy for obtaining highly concentrated phosphorus recovery solution in biofilm phosphorus recovery process[J]. Journal of Environmental Sciences, 2022,112:366-375. [29] 陈淞,苑泉,娄雨晴,等.不同补料方式对好氧颗粒污泥合成PHA的影响[J].中国环境科学, 2024,44(3):1286-1295. Chen S, Yuan Q, Lou Y, et al. Effects of different dosing methods on the synthesis of PHA by aerobic granular sludge[J]. China Environmental Science, 2024,44(3):1286-1295. [30] 王琪,李冬,李鹏垚,等.厌/缺氧时间对好氧颗粒污泥同步硝化内源反硝化和除磷的影响[J].中国环境科学, 2022,42(9):4199-4206. Wang Q, Li D, Li P, et al. Effects of anaerobic/anoxic time on endogenous denitrification and phosphorus removal by synchronized nitrification of aerobic granular sludge[J]. China Environmental Science, 2022,42(9):4199-4206. [31] 杨文焕,邓子威,徐岩,等.光合细菌对活性污泥微生物群落结构及功能的影响[J].中国环境科学, 2024,44(3):1314-1323. Yang W, Dang Z, Xu Y, et al. Effects of photosynthetic bacteria on the structure and function of activated sludge microbial community[J]. China Environmental Science, 2024,44(3):1314-1323. [32] Long X Y, Tang R, Wang T, et al. Characteristics of enhanced biological phosphorus removal (EBPR) process under the combined actions of intracellular and extracellular polyphosphate[J]. Chemosphere, 2021,279:130912. [33] Wang S, Li Z, Wang D, et al. Performance and population structure of two carbon sources granular enhanced biological phosphorus removal systems at low temperature[J]. Bioresource Technology, 2020,300:122683. [34] Carrillo V, Castillo R, Magri A, et al. Phosphorus recovery from domestic wastewater:A review of the institutional framework[J]. Journal of Environmental Management, 2024,351:119812. [35] Zhang C, Guisasola A, Baeza J A. A review on the integration of mainstream P-recovery strategies with enhanced biological phosphorus removal[J]. Water Research, 2022,212:118102. [36] Li H, Zhong Y, Huang H, et al. Simultaneous nitrogen and phosphorus removal by interactions between phosphate accumulating organisms (PAOs) and denitrifying phosphate accumulating organisms (DPAOs) in a sequencing batch reactor[J]. Science of The Total Environment, 2020,744:140852. [37] Yuan J, Deng X, Xie X, et al. Blind spots of universal primers and specific FISH probes for functional microbe and community characterization in EBPR systems[J]. ISME communications, 2024, 4.1:ycae011. [38] Jia L, Cheng X, Fang L, et al. Nitrogen removal in improved subsurface wastewater infiltration system:Mechanism, microbial indicators and the limitation of phosphorus[J]. Journal of Environmental Management, 2023,335:117456. [39] Yan L, Liu S, Liu Q, et al. Improved performance of simultaneous nitrification and denitrification via nitrite in an oxygen-limited SBR by alternating the DO[J]. Bioresource Technology, 2019,275:153-162. [40] Li L, Dong Y, Qian G, et al. Performance and microbial community analysis of bio-electrocoagulation on simultaneous nitrification and denitrification in submerged membrane bioreactor at limited dissolved oxygen[J]. Bioresource Technology, 2018,258:168-176. [41] Song X, Yu D, Qiu Y, et al. Unexpected phosphorous removal in a Candidatus_Competibacter and Defluviicoccus dominated reactor[J]. Bioresource Technology, 2022,345:126540. [42] Ni M, Chen Y, Pan Y, et al. Study on community structure and metabolic mechanism of dominant polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) in suspended biofilm based on phosphate recovery[J]. Science of The Total Environment, 2022,815:152678. [43] Kang D, Yuan Z, Li G, et al. Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater[J]. Environmental Science& Technology, 2023,57.35:13247-13257. [44] Petriglieri F, Singleton CM, Gomez MP, et al. Dechloromonas:to be or not to be a PAO?That is the question![C]//8th IWA Expert Meeting on Microbial Ecology and Water Engineering, Hiroshima, Japan, 2019. [45] Mcllroy SJ, Albertsen M, Andresen EK, et al. Candidatus Competibacter-lineage genomes retrieved from metagenomes reveal functional metabolic diversity[J]. The ISME Journal, 2014,8.3:613-624. [46] Nguyen HTT, Le VQ, Hansen AA, et al. High diversity and abundance of putative polyphosphate-accumulating Tetrasphaera-related bacteria in activated sludge systems[J]. FEMS Microbiology Ecology, 2011, 76.2:256-267. [47] Nielsen PH, Mcllroy SJ, Albertsen M, et al. Re-evaluating the microbiology of the enhanced biological phosphorus removal process[J]. Current Opinion in Biotechnology, 2019,57:111-118. [48] Hou R, Yuan R, Chen R, et al. Metagenomic analysis of denitrifying phosphorus removal in SBR system:comparison of nitrate and nitrite as electron acceptors[J]. Chemical Engineering Journal, 2022,446:137225. [49] Ren T, Chi Y, Wang Y, et al. Diversified metabolism makes novel Thauera strain highly competitive in low carbon wastewater treatment[J]. Water Research, 2021,206:117742. [50] Sabba F, Farmer MK, Jia Z, et al. Impact of operational strategies on a sidestream enhanced biological phosphorus removal (S2EBPR) reactor in a carbon limited wastewater plant[J]. Science of The Total Environment, 2023,857:159280. [51] Zheng Q, Zhang M, Zhang T, et al. Insights from metagenomic, metatranscriptomic, and molecular ecological network analyses into the effects of chromium nanoparticles on activated sludge system[J]. Frontiers of Environmental Science& Engineering, 2020,14:1-11. [52] 安格尔.基于宏基因组的反硝化除磷系统微生物多样性及功能研究[D].呼和浩特:内蒙古大学, 2023. Anger. Macrogenome-based study on microbial diversity and function of denitrification and phosphorus removal system[D]. Hohhot:Inner Mongolia University, 2023. [53] Chen B, Li Y, Luo Z, et al. Formation and granulation mechanism of granular sludge dominated by denitrifying glycogen-accumulating organisms[J]. Chemical Engineering Journal, 2023,474,145638.
