Effect of microbial community on arsenic speciation in sediments of Wuliangsuhai Lake during the ice-bound period
SHI Wen-jing, XU Hao-ran, LIU Yi-zhe, LI Wei-ping
Collaborative Innovation Center of Autonomous Region for Ecological Protection and Comprehensive Utilization in the Inner Mongolia Section of the Yellow River Basin, School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Abstract：To investigate the influence of microbial communities on arsenic speciation in lake sediments of the Hetao Basin in Inner Mongolia during the ice-bound period, the sediments from Wuliangsuhai (WLSH) were taken as the research object. Using 16SrRNA high-throughput sequencing technology, the structural characteristics of microbial communities in WLSH sediments during the ice-bound period were studied. Additionally, methods such as redundancy analysis (RDA), correlation analysis, and co-occurrence network analysis were employed to explore the response relationship between sediment microbial communities and arsenic speciation during the ice-bound period. The results indicated that, apart from the residual arsenic, strongly adsorbed arsenic and arsenic co-precipitated with AVS(Acid-extractable sulfides in sediment), carbonates, manganese oxides, and poorly crystalline Fe hydroxides accounted for a relatively high proportion in the sediments of WLSH during the ice-bound period. When the sedimentary environment was unstable during the ice-bound period, there was a risk of secondary release of arsenic in the sediments of WLSH. The microbial community in the WLSH sediments during the ice-bound period exhibited abundant diversity, and the richness and diversity of microbial community species showed obvious spatial distribution characteristics. There was a significant collinear relationship between microbial communities and arsenic speciation during the ice-bound period, with Thiobacillus, Bacillus, Steroidobacter, Desulfosarcinaceae, and Anaerolinea exhibiting the most pronounced effects on arsenic speciation. Furthermore, adsorbed As, As co-precipitated with AVS, carbonates, manganese oxides, and poorly crystalline Fe hydroxides, as well as As in pyrite, could mutually transform during the ice-bound period, and Thiobacillus and Steroidobacter played crucial roles in this transformation process. This study aims to explore the impact of microbial communities on arsenic speciation in sediments of WLSH during the ice-bound period, providing a microbial theoretical basis and scientific evidence for lake arsenic pollution control. It provides significant implications for the rational development and utilization of water resources, as well as the protection and restoration of aquatic ecological environments.
石文静, 徐浩然, 刘轶哲, 李卫平. 冰封期乌梁素海沉积物微生物群落对砷形态的影响[J]. 中国环境科学, 2025, 45(5): 2724-2734.
SHI Wen-jing, XU Hao-ran, LIU Yi-zhe, LI Wei-ping. Effect of microbial community on arsenic speciation in sediments of Wuliangsuhai Lake during the ice-bound period. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2724-2734.
[1] Rahaman M S, Rahman M M, Mise N et al. Environmental arsenic exposure and its contribution to human diseases, toxicity mechanism and management[J]. Environmental Pollution, 2021,289:117940. [2] 张真,董俊秀,刘晓雯,等.东平湖表层沉积物中砷赋存特征及风险评价[J].环境化学, 2020,39(11):3190-3199. Zhang Z, Dong J X, Liu X W, et al. Arsenic speciation characteristics and risk assessment of surface sediment in Dongping Lake[J]. Environmental Chemistry, 2020,39(11):3190-3199. [3] Yan X P, Kerrich R, Hendry M J, et al. Distribution of arsenic (III), arsenic (V) and total inorganic arsenic in porewaters from a thick till and clay-rich aquitard sequence, Saskatchewan, Canada[J]. Geochimica et Cosmochimica Acta, 2000,64(1):2637-2648. [4] Meharg, A A, Scrimgeour C, Hossain S A, et al. Co-deposition of organic carbon and arsenic in Bengal Delta Aquifers[J]. Environmental Science and Technology, 2006,40(16):4928-4935. [5] 刘碧君,吴丰昌,邓秋静,等.锡矿山矿区和贵阳市人发中锑、砷和汞的污染特征[J].环境科学, 2009,30(3):907-912. Liu B J, Wu F C, Deng Q J, et al. Pollution characteristics of antimony, arsenic and mercury in human hair at Xikuangshan Antimony Mining Area and Guiyang City, China[J]. Environmental Science, 2009,30(3): 907-912. [6] Jain C K, Ali I. Arsenic: occurrence, toxicity and speciation techniques[J]. Water Research, 2000,34(17):4304-4312. [7] 曹洋,孙鹤铭,刘利,等.冬季衡水湖沉积物微生物群落结构特征及影响因素[J].环境工程技术学报, 2023,13(1):154-163. Cao Y, Sun H M, Liu L, et al. Microbial community structure characteristics and influencing factors in sediments of Hengshui Lake in winter[J]. Journal of Environmental Engineering Technology, 2023,13(1):154-163. [8] Yu R L, Yuan X, Zhao Y H, et al. Heavy metal pollution in intertidal sediments from Quanzhou Bay, China[J]. Acta Sedimentologica Sinica, 2008,20(6):664-669. [9] Cheng Y, Zhao F X, Wu J K, et al. Migration characteristics of arsenic in sediments under the influence of cascade reservoirs in Lancang River basin[J]. Journal of Hydrology, 2022,606:127424. [10] Guo T, Li L G, Zhai W W, et al. Distribution of arsenic and its biotransformation genes in sediments from the East China Sea[J]. Environmental Pollution, 2019,253:949-958. [11] Pongratz R. Arsenic speciation in environmental samples of contaminated soil[J]. Science of the Total Environment, 1998,224(1):133-141. [12] 车霏霏,君珊,陈俊伊,等.呼伦湖砷的时空分布特征及成因分析[J].环境科学研究, 2021,34(4):831-840. Che F F, Jun S, Chen J Y, et al. Spatio-temporal distribution and cause analysis of Arsenic in Lake Hulun[J]. Research of Environmental Sciences, 2021,34(4):831-840. [13] 毛凌晨,叶华.氧化还原电位对土壤中重金属环境行为的影响研究进展[J].环境科学研究, 2018,31(10):1669-1676. Mao L C, Ye H. Influence of redox potential on heavy metal behavior in soils: A review[J]. Research of Environmental Sciences, 2018,31(10):1669-1676. [14] 马晟,杨晓莉,刘朝柱,等.三种含钙物质对土壤砷植物有效性的影响[J].中国环境科学, 2022,42(12):5785-5795. Ma S, Yang X L, Liu C Z, et al. Effects of three calcium substances on plant bioavailability of arsenic in soil[J]. China Environmental Science, 2022,42(12):5785-5795. [15] Zhang Z, Moon H S, Scb M, et al. Phosphate enhanced abiotic and biotic arsenic mobilization in the wetland rhizosphere[J]. Chemosphere, 2017,187:130-139. [16] 韩永和,王珊珊.微生物耐砷机理及其在砷地球化学循环中的作用[J].微生物学报, 2016,56(6):901-910. Han Y H, Wang S S. Arsenic resistance mechanisms in microbes and their roles in arsenic geochemical cycling-A review[J]. Acta Microbiologica Sinica, 2016,56(6):901-910. [17] 朱永官,段桂兰,陈保冬,等.土壤-微生物-植物系统中矿物风化与元素循环[J].中国科学:地球科学, 2014,1(6):1107-1116. Zhu Y G, Duan G L, Chen B D, et al. Mineral weathering and element cycling in soil-microorganism-plant system[J]. Science China: Earth Sciences, 2014,57:888–896. [18] Xu L Y,Zhao Z X, et al. Transformation of arsenic in offshore sediment under the impact of anaerobic microbial activities[J]. Water Research, 2011,45:6781-6788. [19] 郑景华,巴楚明,王志宏,等.矿区土壤中砷污染对微生物群落的影响研究[J].地球与环境, 2016,44(5):506-512. Zheng J H, Ba C M, Wang Z H, et al. Influence of arsenic pollution on soil microbial community of mining area[J]. Earth and Environment, 2016,44(5):506-512. [20] Li C M, Lei C X, Liang Y T, et al. As contamination alters rhizosphere microbial community composition with soil type dependency during the rice growing season[J]. Paddy& Water Environment, 2016,15(3): 1-12. [21] Long Y, Jiang J, Hu X, et al. The response of microbial community structure and sediment properties to anthropogenic activities in Caohai wetland sediments[J]. Ecotoxicology and Environmental Safety, 2021,211:111936. [22] 全栋,史小红,赵胜男,等.2006~2017年乌梁素海夏季水体营养状态及影响因子[J].湖泊科学, 2019,31(5):1259-1267. Quan D, SHI X H, Zhao S N, et al. Eutrophication of Lake Ulansuhai in 2006~2017 and its main impact factors[J]. Journal of Lake Sciences, 2019,31(5):1259-1267. [23] 卢兴顺,丁晓宇,赵子闻,等.乌梁素海冰封期污染物分布及迁移特征研究[J].水生态学杂志, 2021,42(6):41-48. Lu X S, Ding X Y, Zhao Z W, et al. Distribution and exchange of pollutants during the icebound season of Ulansuhai Lake[J]. Journal of Hydroecology, 2021,42(6):41-48. [24] Guo H, Tang X, Yang S, et al. Effect of indigenous bacteria on geochemical behavior of arsenic in aquifer sediments from the Hetao Basin, Inner Mongolia: Evidence from sediment incubations[J]. Applied Geochemistry, 2008,23(12):3267-3277. [25] Liu C, Zhao D, Ma W, et al. Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp[J]. Applied Microbiology and Biotechnology, 2016,100(3):1421-1426. [26] Li Y, Yu C, Zhao B, et al. Spatial variation in dissolved phosphorus and interactions with arsenic in response to changing redox conditions in floodplain aquifers of the Hetao Basin, Inner Mongolia[J]. Water Research, 2022,209:177930. [27] Keon N E, Swartz C H, Brabander D J, et al. Validation of an arsenic sequential extr- action method for evaluating mobility in sediments[J]. Environmental Science&Technology, 2001,35(13):2778-2784. [28] Liu C, Zhao D, Ma W, et al. Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp[J]. Applied Microbiology and Biotechnology, 2016,100(3):1421-1426. [29] 石文静,赵心亚,刘轶哲,等.乌梁素海冰封期砷与环境因子响应及风险评价[J].中国环境科学, 2024,44(2):972-983. Shi W J, Zhao X Y, Liu Y Z, et al. Response between arsenic and environmental factors and risk assessment in Wuliangsuhai during the ice-bound period[J]. China Environmental Science, 2024,44(2): 972-983. [30] Hu L, Robert C A M, Cadot S, et al. Root exudate metabolites drive plant-soil feedbacks on growth and defense by shaping the rhizosphere microbiota[J]. Nature Communications, 2018,9(1):2738. [31] 于玲红,齐璐,杨文焕,等.包头南海湖冰封期沉积物细菌群落多样性[J].环境化学, 2019,38(6):1348-1355. Yu L H, Qi L, Yang W H, et al Diversity of bacterial communities in the sediment of Baotou Nanhai Lake in ice period[J]. Environmental Chemistry, 2019,38(6):1348-1355. [32] 王喜宽,黄增芳,苏美霞,等.河套地区土壤基准值及背景值特征[J].岩矿测试, 2007,26(4):287-292. Wang X K, Huang Z F, Su M X, et al. Characteristics of reference and background values of soils in Hetao Area[J]. Rock and Mineral Analysis, 2007,(4):287-292. [33] Che F F, Jiang X, Yao C, et al. Arsenic distribution and speciation in multiphase media of a lake basin, Tibet: The influences of environmental factors on arsenic biogeochemical behavior in the cold arid plateau lake[J]. Science of the Total Environment, 2020,714: 136772. [34] Smith E, Naidu R, Alston A M. Chemistry of inorganic arsenic in soils[J]. Journal of Environmental Quality, 2002,31(2):557-563. [35] Kappen P, Webb J. An EXAFS study of arsenic bonding on amorphous aluminium hydroxide[J]. Applied Geochemistry, 2013,31: 79- 83. [36] Huang J H, Kretzschmar R. Sequential extraction method for specia tion of arsenate and arsenite in mineral soils[J]. Analytical Chemistry, 2010,82(13):5534-5540. [37] Wolthers M, Charlet L, Van Der Weijden C H, et al. Arsenic mobility in the ambient sulfidic environment: Sorption of arsenic (V) and arsenic (III) onto disordered mackinawite[J]. Geochimica et Cosmochimica Acta, 2005,69(14):3483-3492. [38] Postma D, Jessen S, Hue N T M, et al. Mobilization of arsenic and iron from Red River floodplain sediments, Vietnam[J]. Geochimica et Cosmochimica Acta, 2010,74(12):3367-3381. [39] 陈怀满.环境土壤学[M].二版.北京:科学出版社, 2010. Chen H M. Environmental soil science[M]. 2nd Edition. Beijing: Science Press, 2010. [40] 石文静.SRB与DFeRB对砷形态转化的影响机制研究[D].呼和浩特:内蒙古大学, 2020. Shi W J. Study on the influence mechanism of SRB and DFeRB on arsenic species transformation[D]. Hohhot: InnerMongoliaUniversity, 2020. [41] 武斌,廖晓勇,陈同斌,等.石灰性土壤中砷形态分级方法的比较及其最佳方案[J].环境科学学报, 2006,26(9):1467-1473. Wu B, Liao X Y, Chen T B, et al. Comparison of five methods for fractionation of calcareous soil contaminated with arsenic[J]. Acta Scientiae Circumstantiae, 2006,26(9):1467-1473. [42] Xie M, Alsina M A, Yuen J, et al. Effects of resuspension on the mobility and chemical speciation of zinc in contaminated sediments[J]. Journal of Hazardous Materials, 2019,364:300-308. [43] 朱忆雯,尹丹,胡敏,等.稻田土壤氮循环与砷形态转化耦合的研究进展[J].生态环境学报, 2023,32(7):1344-1354. Zhu Y W, Yin D, Hu M, et al. Research progress on coupling of nitrogen cycle and arsenic speciation transformation in paddy soil[J]. Ecology and Environmental Sciences, 32(7):1344-1354. [44] 李嘉义,孙蔚旻,孙晓旭,等.尾矿硫氧化微生物的分离鉴定与功能验证[J].生态环境学报, 2022,31(4):785-792. Li J Y, Sun W M, Sun X X, et al. Isolation, identification and functional verification of sulfur-oxidizing microorganisms in mine tailing[J]. Ecology and Environmental Sciences, 2022,31(4):785-792. [45] 罗文齐,徐梦珊,李丹丹,等.蓝藻暴发对太湖梅梁湾底泥中铁氨氧化速率的影响[J].环境科学学报, 2020,40(8):2828-2833. Luo W Q, Xu M S, Li D D, et al. Effect of cyanobacteria outbreak on Feammox rates in the sediments of Meiliang Bay, Taihu Lake[J]. Acta Scientiae Circumstantiae, 2020,40(8):2828-2833. [46] Smith R L, Kent D B, Repert D A, et al. Anoxic nitrate reduction coupled with iron oxidation and attenuation of dissolved arsenic and phosphate in a sand and gravel aquifer[J]. Geochimica et Cosmochimica Acta, 2017,196:102-120. [47] Asta M P, Wang Y, Frutschi M, et al. Microbially mediated release of as from Mekong Delta Peat sediments[J]. Environmental Science& Technology, 2019,53(17):10208-10217. [48] 王广煊,王鹏程,张琼琼,等.再生水补给湿地香蒲根际细菌群落多样性分析[J].天津师范大学学报(自然科学版), 2017,37(4):46-53. Wang G X, Wang P C, Zhang Q Q, et al. Analysis of rhizosphere bacterial diversity of Typha orientalis in reclaimed water river wetland[J]. Journal of Tianjin Normal University (Natural Science Edition), 2017,37(4):46-53. [49] 杨明,许丽英,宋雨,等.厌氧微生物作用下土壤中砷的形态转化及其分配[J].生态毒理学报, 2013,8(2):178-185. Yang M, Xu L Y, Song Y, et al. Speciation transformation and distribution of arsenic in soils under action of anaerobic microbial activities[J]. AsianJournal of Ecotoxicology, 2013,8(2):178-18.
