Correlation analysis of bacterial community and hypersaline environmental factors in extreme salt lakes on the Qinghai-Tibet Plateau
ZHU De-rui1, HAN Rui2, SHI Qing1, SHEN Guo-ping1, LONG Qi-fu1, SHUANG Jie1
1. Research Center of Basic Medical Sciences, Qinghai University Medical College, Xining 810016, China; 2. Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China
Abstract：The bacterial structure and diversity of extremely salty lakes on the Qinghai-Tibet Plateau were investigated using high-throughput sequencing approach based on 16S rRNA gene (V3~V4region), and then the difference of community compositions and the restrict correlation of hypersaline environmental factors were investigated. Results showed that the bacterial communities of hypersaline samples were relatively stable and evolutionally convergent, and the complexity and the diversity index were significantly lower than the usual salt environment. The dominant bacterial phyla were affiliated to Firmicutes (74.04% to 81.04% of relative abundance proportion), followed by Proteobacteria (15.51% to 20.06%), Bacteroidetes (2.68% to 4.84%) and Actinobacteria (0.71% to 2.45%). Base on the comparative analysis of other types of hypersaline lakes, the characteristic genera in this study were Bacillus (50.63% to 58.35%), Lactococcus (9.28% to 11.81%) and Oceanobacillus (8.41% to 10.52%). Canonical correspondence analysis showed that the dominant bacterial genera (including Bacillus, Lactococcus, Pseudomonas, Oceanobacillus, Stenotrophomonas, Psychrobacter, Myroides, Brochothrix and Arthrobacter) and the hypersaline samples cluster was positively correlated with the saline environment factors including total salinity, Cl-, K+, Mg2+, and CO32-.
朱德锐, 韩睿, 石晴, 沈国平, 龙启福, 双杰. 青藏高原盐湖细菌群落与超盐环境因素的相关性[J]. 中国环境科学, 2017, 37(12): 4657-4666.
ZHU De-rui, HAN Rui, SHI Qing, SHEN Guo-ping, LONG Qi-fu, SHUANG Jie. Correlation analysis of bacterial community and hypersaline environmental factors in extreme salt lakes on the Qinghai-Tibet Plateau. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(12): 4657-4666.
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