Denitrification characteristics of Bacillus subtilis sludge and analysis of microbial community structure
WANG Si-yu1,2, LI Jun1,2, WANG Xiu-jie1,2, WANG Wei-qi1,2, CHAI Jian-zhong3
1. Urban Sewage Depth Processing and Utilization Technology Tational Engieering Laboratory, Beijing University of Technology, Beijing 100124, China; 2. Department of Municipal Engineering, College of Archetecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China; 3. Qinghai Jieshen Environmental Energy Industry Limited Company, Xining 810000, China
Abstract:Compared with the conventional activated sludge, the experiment was carried out to study the denitrification performance of activated sludge of Bacillus subtilis (hereinafter called Bacillus activated sludge) and the effect of Bacillus on microbial community structure. The results showed that within a certain range, the denitrification rate of two sludge was positively correlated with carbon-nitrogen ratio, the maximum denitrification rate of the experimental group could reach 27.144mg TN/(gMLSS·h) with the carbon-nitrogen ratio of 10, about 2.7 times of the control group. The bacterial community structure of the two groups was compared by Miseq high-throughput sequencing, and the results showed that the diversity of bacterial community in the experimental group of bacterial phylum, class, order, family and genus was better than that of the control group. The sludge in the experimental group mainly included Gemmobacter, Brachymonas, Thermomonas, Defluviimonas, Longilinea, Ornatilinea, Aridibacter, Exiguobacterium and so on. These bacteria cooperated to make the activated sludge with high efficient denitrification characteristics with sufficient carbon source.
王思宇, 李军, 王秀杰, 王维奇, 柴建中. 添加芽孢杆菌污泥反硝化特性及菌群结构分析[J]. 中国环境科学, 2017, 37(12): 4649-4656.
WANG Si-yu, LI Jun, WANG Xiu-jie, WANG Wei-qi, CHAI Jian-zhong. Denitrification characteristics of Bacillus subtilis sludge and analysis of microbial community structure. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(12): 4649-4656.
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