Microbial communities differences between aerobic methanotrophs in Miyun Reservoir and North Canal
LIU Yang1,2, CHEN Yong-juan1, WANG Xiao-yan1,3, XU Kang-li1, Yang zhi-wei4
1. College of Resources, Environment and Tourism, Capital Normal University, Beijing 100048, China; 2. China Institute of Water Resources and Hydropower Research, Beijing, 100038, China; 3. Research Center of Aquatic Environment in the Capital Region, Capital Normal University, Beijing 100048, China; 4. Capital Normal University School of Life Sciences, Capital Normal University, Beijing 100048, China
Abstract：The aerobic methanotrophs play an important role in mitigating methane emissions and promoting the carbon-nitrogen cycle in the freshwater ecosystems. In this study, the influences of different water pollutant on aerobic methanotrophs were analyzed between the Miyun Reservoir and the North Canal at the Beijing Metropolitan. The results showed that differences of physiochemical between Miyun Reservoir and the North Canal were significantly, especially the main form of nitrogen. The main form of nitrogen in Miyun Reservoir was NO3--N, while NH4+-N dominated in the North Canal. The physicochemical differences caused significantly influences on the MOB bacterial phylogenetic. Phylogenetic analyses revealed that the upstream freshwater highly contribute to the MOB sequences in Miyun Reservoir, and MOB has closely relationship with NO3--N. However, MOB sequences were mainly from activated sludge and wastewater in the North Canal, and MOB closely related with NH4+-N. The different form of nitrogen in these two ecosystems showed significant influences not only on the MOB serious, but also the sources of MOB. Furthermore, the MOB OTUs showed higher modular microbial network in the North Canal than the Miyun Reservoir. The tightly connected species of MOB communities indicated that bacterial community composition was more vulnerable and sensitive to the various disturbances in the North Canal. The different forms of nitrogen influenced the MOB oxidation and MOB inhibitant activity in different extent, the influence of NH4+-N on MOB was stronger than the influence of NO3--N on the Miyun Reservoir. The high concentration of NH4+-N in the city river that would not only inhibitant the MOB bacteria oxidation but also promote the CH4 release.
刘洋, 陈永娟, 王晓燕, 许康利, 杨志伟. 水库与河流沉积物中好氧甲烷氧化菌群落差异性研究[J]. 中国环境科学, 2018, 38(5): 1844-1854.
LIU Yang, CHEN Yong-juan, WANG Xiao-yan, XU Kang-li, Yang zhi-wei. Microbial communities differences between aerobic methanotrophs in Miyun Reservoir and North Canal. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(5): 1844-1854.
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