1. School of Environmental Science and Engineering, Hebei University of Science & Technology, Shijiazhuang 050000, China;
2. School of Civil Engineering, Hebei University of Science & Technology, Shijiazhuang 050000, China
The distribution characteristics, amplification and correlation of microbial communities and β-lactam resistance genes in active sludge were studied by Miseq sequencing analysis and fluorescence quantitative PCR. The samples of active sludge were obtained from the biological aeration stages of two pharmaceutical wastewater treatment plant (Plant J and K). β-lactam resistance genes OXA-1, OXA-2 and OXA-10can be detected in both plants. The abundance of OXA gene was 5.82×105~3.94×107copies/g (dry weight) in K plant and was 4.84×107~1.09×1010copies/g in J plant. The Abundance of three genes in aeration treatment is significantly amplified. Miseq sequencing showed that the main dominant bacterium were Proteobacteria, Planctomycetes, Bacteroidetes, Chloroflexi and Acidobacteria in K plant and the total average relative abundance ratio was 85.76%. The main dominant bacterium were Proteobacteria, Bacteroidetes, Verrucomicrobia, Gemmatimonadetes and Thermi in J plant, the total average relative abundance ratio was 85.76%. Redundancy analysis display showed that Bdellovibrio、KD8-87 and Paracoccus in biological community were the major distribution factors of OXA-1、OXA-2 and OXA-10. Hyphomicrobium、Thermomonas and Comamonadaceae were the major distribution factor of OXA-1. Caldilineaceae、Myxococcales and Pirellulaceae were the major distribution factor of OXA-10.
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