氨氮和硫酸盐对谷氨酸厌氧生物降解性能的抑制及机理

陈宏; 胡颖冰; 陈晨; 韦燕霄; 黄蓉; 王泓; 余关龙; 杨敏

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中国环境科学 ›› 2020, Vol. 40 ›› Issue (10) : 4342-4347.

水污染与控制

氨氮和硫酸盐对谷氨酸厌氧生物降解性能的抑制及机理

陈宏^1,2, 胡颖冰^1,2, 陈晨³, 韦燕霄^1,2, 黄蓉^1,2, 王泓^1,2, 余关龙^1,2, 杨敏²

作者信息 +

Inhibition and process mechanism of ammonia nitrogen and sulfate on anaerobic degradation of glutamate

CHEN Hong^1,2, HU Ying-bing^1,2, CHEN Chen³, WEI Yan-xiao^1,2, WANG Hong^1,2, HUANG Rong^1,2, YU Guan-long^1,2, YANG Min²

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摘要

采用连续运行1119d的上流式厌氧污泥床（UASB）反应器，研究了最佳有机负荷条件下氨氮和硫酸盐对模拟废水中谷氨酸降解性能的抑制作用.结果表明，有机负荷为8.0g COD/（L·d）时，COD去除率达到最高值为（97.94±0.28）%.逐步提高进水氨氮浓度，起初对谷氨酸降解性能的影响不大；但升到2000mg/L时COD去除率和甲烷产率明显降低，继续升至4000mg/L时即达到半抑制状态.逐步提高进水硫酸盐浓度至4000mg/L，甲烷产率和溶液中游离硫化氢（FS）浓度分别呈现一直下降和升高趋势，但COD去除率均能维持在90%以上.进水中的氨氮和硫酸盐分别因离解平衡和生物还原作用形成游离氨（FAN）和FS，进而抑制了产甲烷菌的活性；前者因FAN扩散到细胞内部破坏质子平衡从而过多消耗ATP，后者还因硫酸盐还原菌的增殖存在底物竞争抑制作用.

Abstract

To investigate the effect of ammonia nitrogen and sulfate in the influent on anaerobic degradation of glutamate under the optimal organic loading rate, a lab-scale up-flow anaerobic sludge blanket (UASB) reactor was continuously operated for 1119 days. The results showed that the maximal COD removal efficiency as high as (97.94±0.28)% was obtained at an organic loading rate of 8.0g COD/(L·d). When step-wise increasing the ammonia nitrogen concentration in the influent, the UASB reactor a small fluctuation on glutamate removal efficiency under low ammonia nitrogen concentrations (<1000mg/L), subsequently appeared a distinct decline in the COD removal and methane yield at 2000mg/L, and then the half-inhibitory concentration of ammonia nitrogen exhibited at 4000mg/L. When step-wise increasing sulfate concentration in the influent from 200mg/L to 4000mg/L, methane yield and free sulfide (FS) concentration in the mixture fallen into increasing and decreasing trends, respectively; but the COD removal efficiencies were generally in a high level (>90%). Both the ammonia nitrogen and sulfate in the influent could inhibit the glutamate degradation activity of methane-producing archaea (MPA) through dissociation equilibrium and biological reduction, respectively. Besides, FAN could freely diffuse into microbial cells resulting in proton imbalance and then excessive ATP consumption in the former. In contrast, substrate competitive inhibition was existed as for the reproduction of sulfate-reducing bacteria in the latter.

导出引用

陈宏, 胡颖冰, 陈晨, 韦燕霄, 黄蓉, 王泓, 余关龙, 杨敏. 氨氮和硫酸盐对谷氨酸厌氧生物降解性能的抑制及机理[J]. 中国环境科学. 2020, 40(10): 4342-4347

CHEN Hong, HU Ying-bing, CHEN Chen, WEI Yan-xiao, WANG Hong, HUANG Rong, YU Guan-long, YANG Min. Inhibition and process mechanism of ammonia nitrogen and sulfate on anaerobic degradation of glutamate[J]. China Environmental Science. 2020, 40(10): 4342-4347

中图分类号： X703

参考文献

[1] Li J, Rui J, Yao M, et al. Substrate type and free ammonia determine bacterial community structure in full-scale mesophilic anaerobic digesters treating cattle or swine manure[J]. Frontiers in Microbiology, 2015,6:1337.
[2] 刘敏,任南琪,王爱杰,等.UASB反应器酸化后的状态及恢复研究[J]. 中国沼气, 2003,(2):7-10. Liu M, Ren N Q, Wang A J, et al. Acidification State and Recovery Experiment of UASB Reactor[J]. China Biogas, 2003,(2):7-10.
[3] Zhang S J, Liu N R, Zhang C X. Study on the performance of modified UASB process treating sewage[J]. Advanced Materials Research, 2013,2115(1223):2174-2178.
[4] 丁忠浩,蔡连浪,李镭,等.用上流式厌氧污泥床处理味精废水的研究[J]. 环境科学与技术, 2002,(4):30-31. Ding H J, Cai L L, Li L, et al. Experimental studies of monosodium glutamate effluents using UASB[J]. Environmental Science & Technology, 2002,(4):30-31.
[5] Gao Y. UASB treatment of monosodium glutamate wastewater. The 4th International Conference on Bioinformatics and Biomedical Engineering[Z]. 2010.
[6] Chen H, Wei Y, Liang P, et al. Performance and microbial community variations of a upflow anaerobic sludge blanket (UASB) reactor for treating monosodium glutamate wastewater:Effects of organic loading rate[J]. Journal of Environmental Management, 2020,253:109691.
[7] 国家环境保护总局编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002. Editorial Board of The State Environmental Protection Administration. Methods for monitoring and analysis of water and wastewater[M]. 4 version. Beijing:China Environmental Science Press, 2002.
[8] 孙冬月,官香元.重铬酸钾法测定COD中的干扰及消除[J]. 环境科学与管理, 2009,34(6):124-126. Sun D Y, Guan X Y, Elimination of interference on COD measurement by K₂Cr₂O₇ method[J]. Environmental Science and Management, 2009,34(6):124-126.
[9] Jiang Y, McAdam E, Zhang Y, et al. Ammonia inhibition and toxicity in anaerobic digestion:A critical review[J]. Journal of Water Process Engineering, 2019,32:100899.
[10] Omil F, Mendez R, Guerrero L. Anaerobic hydrolysis and acidogenesis of wastewaters from food industries with high content of organic solids and protein[J]. Water Research:A Journal of the International Water Association, 1999,33(12):3281-3290.
[11] Onay T T, Demirel B, Yenigun O. Anaerobic treatment of dairy wastewaters:a review[J]. Process Biochemistry, 2005,40(8):2583-2595.
[12] 乔玮,毕少杰,熊林鹏,等.氨氮浓度对鸡粪中高温甲烷发酵的影响[J]. 中国环境科学, 2019,39(7):2921-2927. Qiao W, Bi S J, Xiong L P, et al. Effects of ammonium onmethane fermentation of chicken manure under mesophilic and thermophilic conditions[J]. China Environmental Science, 2019,39(7):2921-2927.
[13] Lu X, Zhen G, Ni J, et al. Sulfidogenesis process to strengthen re-granulation for biodegradation of methanolic wastewater and microorganisms evolution in an UASB reactor[J]. Water Research, 2017,108:137-150.
[14] 陈泓,王雯,严湖,等.氨氮对有机废弃物厌氧消化的影响及调控策略[J]. 环境科学与技术, 2016,39(9):88-95. Chen H, Wang W, Yan H, et al. Ammonia inhibition on anaerobic digestion and control strategy:A review[J]. Environmental Science & Technology, 2016,39(9):88-95.
[15] Heo N P S C K. Effects of mixture ratio and hydraulic retention time on single-stage anaerobic co-digestion of food waste and waste activated sludge[J]. Journal of Environmental Science and Health, 2004,39(7):1739-1756.
[16] Wang W, Ren X, Yang K, et al. Inhibition of ammonia on anaerobic digestion of synthetic coal gasification wastewater and recovery using struvite precipitation[J]. Journal of Hazardous Materials, 2017,340:152-159.
[17] MácA J, Dohányos M, Dolej P, et al. Stability and inhibition of anaerobic processes caused by insufficiency or excess of ammonia nitrogen[J]. Applied Microbiology and Biotechnology, 2012,93(1):439-447.
[18] 孟伟,查金,张思梦,等.餐厨垃圾厌氧消化过程氨氮抑制及缓解办法综述[J]. 环境工程, 2019,37(12):177-182. Meng J, Zha J, Zhang S M, et al. A review of ammonia inhibition and its mitigation methods for anaerobic digestion of food waste[J]. Environmental Engineering, 2019,37(12):177-182.
[19] Chen S, He J, Wang H, et al. Microbial responses and metabolic pathways reveal the recovery mechanism of an anaerobic digestion system subjected to progressive inhibition by ammonia[J]. Chemical Engineering Journal, 2018,350:312-323.
[20] Yan M, Fotidis I A, Tian H, et al. Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels:Focusing on microbial community dynamics[J]. Bioresource Technology, 2019,286:121376.
[21] 胡崇亮,张栋,戴翎翎,等.厌氧消化过程氨抑制研究进展[J]. 环境工程, 2016,34(12):23-27. Hu C L, Zhang D, Dai L L, et al. Research progress of inhibitory effects of ammonia in an anaerobic dogestion system[J]. Environmental Engineering, 2016,34(12):23-27.
[22] Maree J P, Strydom W F. Biological sulphate removal from industrial effluent in an upflow packed bed reactor[J]. Water Research, 1987, 21(2):141-146.
[23] Chen H, Wu J, Liu B, et al. Competitive dynamics of anaerobes during long-term biological sulfate reduction process in a UASB reactor[J]. Bioresource Technology, 2019,280:173-182.
[24] 苗妍,王志伟,梅晓洁,等.基质配比及硫酸盐对AnMBR产甲烷性能的影响[J]. 中国环境科学, 2017,37(3):998-1002. Miao Y, Wang Z W, Mei X J, et al. Effects of substrate ratios and sulfates on methanogenesis in an AnMBR[J]. China Environmental Science, 2017,37(3):998-1002.