1. School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China;
2. State Environmental Protection Key Laboratory of Urban Ecological Environment Simulation and Protection, South China Institute of Environmental Sciences, Ministry of Ecology and Environmental, Guangzhou 510535, China;
3. Zheng Ye Design CO. LTD, Harbin 150000, China
In order to study the species diversity on the surface of filter material in an ecological aerated filter (EAF) and elucidate its water purification mechanism, microbial abundance and diversity in different dissolved oxygen (DO) regions of two fillers in the EAF were analyzed using high-throughput sequencing and bioinformatics analysis. Proteobacteria was the predominant specie which accounted for more than 71% during the EAF operation. The large proliferation of dominant bacteria enabled the reactorexhibited a high TN removal rate (67%), The ratio of dominant bacteria on the surface samples of sponge iron in aerobic area was the highest(77.49%), indicating that sponge iron was more suitable for the propagation of proteobacteria of dominant bacteria under aeration conditions. Alpha diversity analysis found that the abundance of microbial colonies in the biofilmof the filler surface of EAF was higher than that of the control group, indicating that EAF was more suitable for the growth of microorganisms than the natural environment. The diversity analysis of Beta showed that the aeration in the reactor caused the dominant bacteria to propagate effectively on the filler surface, which suppressed the growth of other bacteria. Although the biodiversity declined slightly, the removal capacity of total nitrogen (TN) was improved with the proliferation of the dominant bacteria.
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