Study on microbial surface hydrophobicity under multiphase interface of water distribution network
ZHANG Ming-lu1, XU Shao-feng1, XU Meng-yao1, WANG Li1, CHAI Shan-shan1, BAI Miao2, ZHANG Can2
1. Department of Environmental Science and Engineering, Beijing Technology and Business University, Beijing 100048, China; 2. Center for Disease Control and Prevention of Chinese PLA, Beijing 100071, China
Abstract：The CDC (Centers for Disease Control) biofilm reactor was used to simulate the water distribution system, and two kinds of hanging materials of polyvinyl chloride (PVC) and polycarbonate (PC) were selected. The cell hydrophobicity of different bacterial populations in the water phase, biofilm phase and particulate phase of the two hanging materials was studied by a combination of microbial adhesion hydrocarbon (MATH) experiment and Illumina high-throughput sequencing. The results showed that the dominant bacteria in the reactor of PVC hanging coupon was Firmicutes and the relative abundance was 68.31%~81.00%. While the dominant bacteria in the reactor of PC hanging coupon was Proteobacteria and the relative abundance was 24.39%~64.40%. The predominant bacteria in the reactor of PVC hanging coupon contained three types of pathogenic bacteria, and the PC hanging coupon contained eight pathogens. The hydrophobicity of biofilm phase on the PC hanging coupon was higher than that on PVC, which facilitated the attachment of bacteria and biofilm formation. The ability of PVC material to form biofilm was lower. Hence, it had positive impact on the secondary pollution control during the water transportation. However, other engineering factors should also be considered in the practical applications.
张明露, 徐绍峰, 徐梦瑶, 王礼, 柴杉杉, 白淼, 张灿. 给水管网多相界面中微生物表面疏水性研究[J]. 中国环境科学, 2019, 39(11): 4823-4830.
ZHANG Ming-lu, XU Shao-feng, XU Meng-yao, WANG Li, CHAI Shan-shan, BAI Miao, ZHANG Can. Study on microbial surface hydrophobicity under multiphase interface of water distribution network. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(11): 4823-4830.
Power K N, Nagy L A. Relationship between bacterial regrowth and some physical and chemical parameters within Sydney's drinking water distribution system[J]. Water Research, 1999,33(3):741-750.
[2]
林文芳,余志晟,陈曦,等.给水管网生物膜反应器及分子生物学研究方法进展[J]. 环境科学与技术, 2012,35(6):71-78. Lin W F, Yu Z C, Chen X, et al. Advances in research methods of biofilm reactors and molecular biology in water supply networks[J] Environmental Science & Technology, 2012,35(6):71-78.
[3]
周玲玲,刘文君,张永吉.给水管壁生物膜附着异养菌与水中悬浮菌活性比较[J]. 中国给水排水, 2007,23(21):37-40. Zhou L L, Liu W J, Zhang Y J. Comparison of activity of heterotrophic bacteria attached to biofilm of water supply wall and suspended bacteria in water[J]. China Water & Wastewater, 2007,23(21):37-40.
[4]
陈雨乔,段晓笛,陆品品,等.给水管网中耐氯性细菌的灭活特性研究[J]. 环境科学, 2012,33(1):104-109. Chen Y Q, Duan X D, Lu P P, et al. Study on inactivation characteristics of chlorine-tolerant bacteria in water supply network[J]. Environmental Science, 2012,33(1):104-109.
[5]
张向谊,刘文君,高圣华,等.模拟配水管网中管材和余氯对生物膜形成的影响[J]. 中国环境科学, 2006,(3):303-306. Zhang X Y, Liu W J, Gao S H, et al. The influence of pipe materials and residual chlorine in the stimulant water distribution pipe net on the biofilm formation.[J]. China Environmental Science, 2006,(3):303-306.
[6]
叶萍,申屠华斌,陈环宇,等.滞流工况下管网水中微生物群落对铁释放的影响[J]. 中国环境科学, 2017,37(12):4578-4584. Ye P, Shen T H B, Chen H Y, et al. Influence of microbial community on iron release under stagnation condition in drinking water systems[J]. China Environmental Science, 2017,37(12):4578-4584.
[7]
孙慧芳,石宝友,王东升.供水管网内壁生物膜的特征及其对水质的影响[J]. 中国给水排水, 2011,27(21):40-45. Sun H F, Shi B Y, Wuang D S. Characteristics of biofilm on the inner wall of water supply network and its influence on water quality[J]. China Water & Wastewater, 2011,27(21):40-45.
[8]
黄翔峰.MATH法表征环境微生物细胞表面疏水性的研究进展[J]. 微生物学通报, 2015,42(1):200-206. Huang X F. Advances in MATH Method for characterizing the surface hydrophobicity of environmental microorganisms[J]. Microbiology China, 2015,42(1):200-206.
[9]
Popovici J, White C P, Hoelle J, et al. Characterization of the cell surface properties of drinking water pathogens by microbial adhesion to hydrocarbon and electrophoretic mobility measurements[J]. Colloids & Surfaces B:Biointerfaces, 2014,118:126-132.
[10]
Noriyuki I, Michio S, Hirosi Anzai, et al. Relationships among colony morphotypes, cell-surface properties and bacterial adhesion to substrata in Rhodococcus[J]. Colloids & Surfaces B Biointerfaces, 2003,30(1):51-60.
[11]
Djeridi I, Cécile Militon, Grossi V, et al. Evidence for surfactant production by the haloarchaeon Haloferax, sp. MSNC14 in hydrocarbon-containing media[J]. Extremophiles, 2013,17(4):669-675.
[12]
Zhang M L, Wang L, Xu M Y, et al. Selective antibiotic resistance genes in multiphase samples during biofilm growth in a simulated drinking water distribution system:Occurrence, correlation and low-pressure ultraviolet removal[J]. Science of The Total Environment, 2019,649:146-155.
[13]
Caporaso J G, Lauber C L, Walters W A, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample[J]. Proceedings of the National Academy of Sciences, 2011,108Suppl 1(Supplement_1):4516-4522.
[14]
Yuan Q C, Feng G, Herbert H P, et al. Hydrophobicity of diverse bacterial populations in activated sludge and biofilm revealed by microbial adhesion to hydrocarbons assay and high-throughput sequencing[J]. Colloids & Surfaces B:Biointerfaces, 2014,(114):379-385.
[15]
Busscher H J, Mei H C V D. Implications of microbial adhesion to hydrocarbons for evaluating[J]. Colloids & Surfaces B Biointerfaces, 1995,5(3/4):111-116.
[16]
Rosenberg M. Microbial adhesion to hydrocarbons:Twenty-five years of doing MATH[J]. FEMS Microbiology Letters, 2006,262(2):129-134.
[17]
Vaz-Moreira I, Nunes O C, Manaia, et al. Ubiquitous and persistent, Proteobacteria, and other Gram-negative bacteria in drinking water[J]. Science of the Total Environment, 2017,586:1141-1149.
[18]
Wu H, Zhang J, Mi Z, et al. Biofilm bacterial communities in urban drinking water distribution systems transporting waters with different purification strategies[J]. Applied Microbiology & Biotechnology, 2015,99(4):1947-1955.
[19]
Sun H, Shi B, Bai Y, et al. Bacterial community of biofilms developed under different water supply conditions in a distribution system[J]. Science of The Total Environment, 2014,472:99-107.
[20]
Marciano-Cabral F, Jamerson M, Kaneshiro E S. Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA[J]. Journal of Water & Health, 2010,8(1):71-82.
[21]
Gomez-Smith C K, Lapara T M, Hozalski R M. Sulfate reducing bacteria and Mycobacteria dominate the biofilm communities in a chloraminated drinking water distribution system[J]. Environmental Science & Technology, 2015,49(14):8432-8440.
[22]
Narciso-Da-Rocha C, Vaz-Moreira I, Svensson-Stadler L, et al. Diversity and antibiotic resistance of Acinetobacter spp. in water from the source to the tap[J]. Applied Microbiology & Biotechnology, 2013, 97(1):329-340.
[23]
Kelly J J, Minalt N, Culotti A, et al. Temporal variations in the abundance and composition of biofilm communities colonizing drinking water distribution pipes[J]. PLoS One, 2014,9(5):1-11.
[24]
张明露,刘文君,李翠萍,等.水源切换对水厂出水细菌群落的影响[J]. 中国环境科学, 2015,35(8):2517-2522. Zhang M L, Liu W J, Li C P, et al. Effect of water source switching on bacterial community in effluent of waterworks[J]. China Environmental Science, 2015,35(8):2517-2522.
[25]
Liao X, Chen C, Wang Z, et al. Bacterial community change through drinking water treatment processes[J]. International Journal of Environmental Science & Technology, 2015,12(6):1867-1874.
[26]
Flores Ribeiro A, Bodilis J, Alonso L, et al. Occurrence of multi-antibiotic resistant Pseudomonas spp. in drinking water produced from karstic hydrosystems[J]. Science of The Total Environment, 2014, 490:370-378.
[27]
胡子鉴.不动杆菌属感染的研究进展[J]. 医学综述, 1999,(1):5-7. Hu Z J. Advances in research on Acinetobacter infection[J]. Medical Recapitulate, 1999,(1):5-7.
[28]
O'Brien K L, Wolfson L J, Watt J P, et al. Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years:Global estimates[J]. The Lancet, 2009,374(9693):893-902.
