1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Gansu Sewage Treatment Industry Technical Center, Lanzhou 730070, China;
3. Lanzhou Urban Water Supply(Group) co. LTD, Lanzhou 730070, China;
4. Sun Yat-sen University, Guangzhou 510000, China;
5. South China Institute of Environmental Science, Guangzhou 510655, China;
6. School of Environment and Material Engineering, Yantai University, Yantai 264005, China
A sequencing batch reactor (SBR) was operated in this study to investigate the effect of pH on the kinetics of NOB activity.For revealing the details the activated sludge abundant with NOB was used as an object and several statistics analyses based on Monod model were also applied. The results showed that the Monod equation fitted the experimental data well, and the greatest NOB activity was achieved as Ks=6.167mg/L, rmax=1.13g/(g·d) under pH=7.0.The analyses of bell-shaped empirical model disclosed that rmax varied in a bell shape as pH value increased. The optimum pH was 6.9±0.1whilethe rmax maintained above a half of the greatest rmax(ropt) was 3.26±0.4. The real-time PCR analysis results showed that the variation trend of nxrA gene and nxrB gene is consistent with the rule of kinetic parameters, and both nxrA and nxrB genes worked in the degradation process of the system.
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