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pH值对亚硝酸盐氧化菌动力学及功能基因的影响
Effects of pH on the kinetics of NOB and functional genes
为探究pH值对亚硝酸盐氧化菌（NOB）活性动力学影响，本试验采用序批式活性污泥（SBR）反应器，以富含NOB的活性污泥为对象，基于Monod模型考察不同pH值对NOB活性动力学的影响并进行统计学分析.结果表明，Monod方程可较好地反映不同pH值条件下基质底物浓度对NOB比亚硝态氮氧化速率（SNiOR）的影响，且pH=7.0时动力学参数Ks为（6.167mg/L），rmax为[1.134g/（g·d）]，此时NOB活性最好.利用钟形经验模型进行非线性回归拟合，最大比降解速率（rmax）随pH值的增大呈钟形变化，本试验NOB的最佳pH值为（6.9±0.1），其中rmax维持在ropt一半以上的pH值范围（ω）为（3.26±0.4）.以亚硝酸盐氧化还原酶类基因（nxrA、nxrB）为引物，基于荧光定量PCR技术分析结果显示，在不同pH值条件下nxrA基因和nxrB基因拷贝数的变化趋势均与动力学参数（Ks、rmax）的规律一致，且nxrA和nxrB基因在系统的降解过程中起协同作用.
A sequencing batch reactor (SBR) was operated in this study to investigate the effect of pH on the kinetics of NOB activity.For revealing the details the activated sludge abundant with NOB was used as an object and several statistics analyses based on Monod model were also applied. The results showed that the Monod equation fitted the experimental data well, and the greatest NOB activity was achieved as Ks=6.167mg/L, rmax=1.13g/(g·d) under pH=7.0.The analyses of bell-shaped empirical model disclosed that rmax varied in a bell shape as pH value increased. The optimum pH was 6.9±0.1whilethe rmax maintained above a half of the greatest rmax(ropt) was 3.26±0.4. The real-time PCR analysis results showed that the variation trend of nxrA gene and nxrB gene is consistent with the rule of kinetic parameters, and both nxrA and nxrB genes worked in the degradation process of the system.
pH值 / 动力学 / 亚硝酸盐氧化菌 / 荧光定量PCR / 钟形经验模型
bell-shaped empirical model / kinetic / NOB / pH / real-time PCR
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国家自然科学基金资助项目（51668031）；深圳市科技计划项目（JSGG20170824101458515）
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