1. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China;
2. School of Civil Engineering, Dalian Nationalities University, Dalian 116600, China;
3. Jiangsu Tianyu Environmental protection Group CO. Ltd., Yangzhou 225200, China
In order to analyze the effect of salinity on NOB (nitrite-oxidizing bacteria) activity and kinetic characterization, NOB was enriched in a lab-scale SBR system with high concentration of nitrite wastewater in this study. The FISH (fluorescence in situ hybridization) analysis showed that Nitrobacter accounted for(81%±6%)of total bacteria. The maximum specific nitrite oxidation rate was (42.5±0.9)mgN/(gVSS·h). The effect of salinity on NOB activity was investigated using this mixed culture. The kinetic parameters of NOB (Ko, KS) were both measured under salinity of 10g/L. Results showed that compared with salinity 0g/L, the activity of NOB decreased 3.3% unders alinity of 15g/L and decreased 11% under salinity of 10, 20g/L. When salinity was 10g/L, the maximum specific nitrite oxidation rate was (37.9±0.7)mgN/(gVSS·h). The half-saturation constant for oxygen (Ko) and nitrite (KS) were (1.51±0.06), (6.06±0.15) mg/L. The values of Ko and KS were higher than recommended value of ammonia-oxidizing bacteria (AOB) in ASM2model. It is suggested that salinity could reduce maximum specific nitrite oxidation rate and affect the oxygen affinity and substrate (nitrite) affinity of NOB.
崔有为,王淑莹,朱岩,等.海水代用及其含盐污水的生物处理[J]. 工业水处理, 2005,25(10):6-10. Cui Y W, Wang S Y, Zhu Y, et al. Seawater substitute and bio-treatment of saline wastewater[J]. Industrial Water Treatment, 2005, 25(10):6-10.
[2]
Lefebvre O, Moletta R. Treatment of organic pollution in industrial saline wastewater:A literature review[J]. Water Research, 2006, 40(20):3671-3682.
[3]
刘甜甜,彭永臻,王淑莹,等.盐度对垃圾渗滤液短程脱氮性能及其N2O产量的影响[J]. 化工学报, 2012,63(10):3269-3276. Liu T T, Peng Y Z, Wang S Y, et al. Impact of salinity shock on nitrogen removal and N2O output in treating landfill leachate[J]. CIESC Journal, 2012,63(10):3269-3276.
[4]
Li Y, Wang Z, Li J, et al. Inhibition kinetics of nitritation and half-nitritation of old landfill leachate in a membrane bioreactor[J]. Journal of Bioscience and Bioengineering, 2017,123(4):482-488.
[5]
崔有为,丁洁然,陈叶菲,等.盐度入侵城市污水处理厂对淡水活性污泥的毒性抑制[J]. 化工学报, 2012,63(5):1566-1573. Cui Y W, Ding J R, Chen Y F, et al. Assessment on effect of salt toxicity on activity of activated sludge in wastewater treatment plant[J]. CIESC Journal, 2012,63(5):1566-1573.
[6]
Corsino S F, Capodici M, Di Pippo F, et al. Comparison between kinetics of autochthonous marine bacteria in activated sludge and granular sludge systems at different salinity and SRTs[J]. Water Research, 2019,148:425-437.
[7]
Chen X, Wang X, Chen X, et al. Salt inhibition on partial nitritation performance of ammonium-rich saline wastewater in the zeolite biological aerated filter[J]. Bioresource Technology, 2019,280:287-294.
[8]
王淑莹,唐冰,叶柳,等. NaCl盐度对活性污泥系统脱氮性能的影响[J]. 北京工业大学学报, 2008,6:631-635. Wang S Y, Tang B, Ye L, et al. Influence of NaCI salinity on nitrogen removal of activated sludge system[J]. Journal of Beijing University of Technology, 2008,6:631-635.
[9]
Ye L, Peng C, Tang B, et al. Determination effect of influent salinity and inhibition time on partial nitrification in a sequencing batch reactor treating saline sewage[J]. Desalination, 2009,246:556-566.
[10]
Moussa M S, Sumanasekera D U, Ibrahim S H, et al. Long term effects of salt on activity, population structure and floc characteristics in enriched bacterial cultures of nitrifiers[J]. Water Research, 2006, 40(7):1377-1388.
[11]
Macêdo W V, Santos C E D, Guerrero R D B S, et al. Establishing simultaneous nitrification and denitrification under continuous aeration for the treatment of multi-electrolytes saline wastewater[J]. Bioresource Technology, 2019,288:121529.
[12]
胡雪柳,李淑更,张立秋,等.SBR短程硝化系统处理垃圾渗滤液及盐度抑制效应[J]. 工业水处理, 2018,38(12):35-38. Hu X L, Li S G, Zhang L Q, et al. Treatment of landfill leachate by SBR shortcut nitrification system and the salinity inhibition effects[J]. Industrial Water Treatment, 2018,38(12):35-38.
[13]
张兰河,田蕊,陈子成,等.NaCl盐度对A~2/O工艺去除废水污染物和系统微生物的影响[J]. 农业工程学报, 2018,34(10):231-237. Zhang L H, Tian R, Chen Z C, et al. Effects of NaCl salinity on wastewater pollutants removal and microorganism in A2/O technology process[J]. Transactions of the Chinese Society of Agricultural Engineering, 2018,34(10):231-237.
[14]
张彬彬,田凤蓉,杨志林,等. NaCl盐度对耐盐脱氮污泥硝化功能的影响[J]. 环境工程学报, 2014,8(8):3265-3269. Zhang B B, Tian F R, Yang Z L, et al. Effects of salinity on nitrification in salt-tolerant denitrifying sludge system[J]. Chinese Journal of Environmental Engineering, 2014,8(8):3265-3269.
[15]
Uygur A, Karg F. Salt inhibition on biological nutrient removal from saline wastewater in a sequencing batch reactor[J]. Enzyme and Microbial Technology, 2004,34(3/4):313-318.
[16]
Henze M G W M T. Activated sludge models ASM1, ASM2, ASM2d and ASM3scientific and technical report No.9[Z]. London:IWA Publishing, 2000.
[17]
Manser R, Gujer W, Siegrist H. Consequences of mass transfer effects on the kinetics of nitrifiers[J]. Water Research, 2005,39(19):4633-4642.
[18]
Roots P, Wang Y, Rosenthal A F, et al. Comammox Nitrospira are the dominant ammonia oxidizers in a mainstream low dissolved oxygen nitrification reactor[J]. Water Research, 2019,157:396-405.
[19]
Yu L, Li R, Hua S, et al. Reactor configuration on the nitrifier community and kinetics of activated sludge process[J]. Desalination and Water Treatment, 2018,123:82-89.
[20]
Vadivelu V M, Yuan Z, Fux C, et al. Stoichiometric and kinetic characterisation of Nitrobacter in mixed culture by decoupling the growth and energy generation processes[J]. Biotechnology and bioengineering, 2006,94(6):1176-1188.
[21]
Blackburne R, Vadivelu V M, Yuan Z, et al. Kinetic characterisation of an enriched Nitrospira culture with comparison to Nitrobacter[J]. Water Research, 2007,41(14):3033-3042.
[22]
Apha. Standard methods for the examination of water and wastewater[M]. 20th ed. ed. Washington DC, USA:merican Water Works Association and Water Environment Federation, 1998.
[23]
Beun J J V L M C. Aerobic granulation[J]. Water Science and Technology, 2000,4-5(41):41-48.
[24]
Amann R I, Krumholz L, Stahl D A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology[J]. Journal of Bacteriology, 1990,172(2):762-770.
[25]
Ge S J, Wang S Y, Yang X, et al. Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment:A review[J]. Chemosphere, 2015,140:85-98.
[26]
Gu S B. Study on optimizing short-cut nitrification process in a pilot-scale sequencening batch reactor based on microbial population and process regulation plus kinetic[D]. Beijing:Beijing University of Tecnology, 2012.
[27]
Vadivelu V M, Keller J, Yuan Z. Stoichiometric and kinetic characterisation of Nitrosomonas sp. in mixed culture by decoupling the growth and energy generation processes[J]. Journal of Biotechnology, 2006,126(3):342-356.
[28]
Vadivelu V M, Keller J, Yuan Z. Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture[J]. Water Research, 2007,41(4):826-834.
[29]
崔有为,王淑莹,宋学起,等.NaCl盐度对活性污泥处理系统的影响[J]. 环境工程, 2004,1:19-21. Cui Y W, Wang S Y, Song X Q, et al. Effects of NaCl salinity on activated sludge treatment system.[J]. Environmental engineering, 2004,1:19-21.
[30]
张宇坤,王淑莹,董怡君,等.NaCl盐度对氨氧化细菌活性的影响及动力学特性[J]. 中国环境科学, 2015,35(2):465-470. Zhang Y K, Wang S Y, Dong Y J, et al. Effect of NaCl salinity on activity of ammonia-oxidizing bacteria and kinetic characterisation[J]. China Environmental Science, 2015,35(2):465-470.
[31]
Park M, Park H, Chandran K. Molecular and Kinetic Characterization of PlanktonicNitrospira spp. Selectively Enriched from Activated Sludge[J]. Environmental Science and Technology, 2017,51(5):2720-2728.
